AB134556

Anti-p46 antibody

Name: Anti-p46 antibody (ab134556) | Abcam
Brand: Abcam Limited
SKU: AB134556
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal p46 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLDIP3 aa 350 to C-terminus.

View Alternative Names

KIAA1649, PDIP46, POLDIP3, Polymerase delta-interacting protein 3, 46 kDa DNA polymerase delta interaction protein, S6K1 Aly/REF-like target, p46, SKAR

2 Images

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Immunoprecipitation - Anti-p46 antibody (AB134556)

Detection of p46 by Western blot of immunprecipitate.
Lane 1 : ab134556, at a 1 μg/ml, staining p46 in HeLa whole cell lysate immunoprecipitated using ab134556 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : The lysate is precipitated with an IgG control antibody.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-p46 antibody (ab134556)

Predicted band size: 46 kDa

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Western blot - Anti-p46 antibody (AB134556)

All lanes:

Western blot - Anti-p46 antibody (ab134556) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 46 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human POLDIP3 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BY77

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab134556 is affinity purified using an epitope specific to p46 immobilized on a solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P46 also known as heat shock protein 46 (HSP46) is a molecular chaperone within the heat shock protein family. It maintains a mass of approximately 46 kDa and is ubiquitously expressed in various tissues. The protein assists in protein folding and prevents the aggregation of misfolded proteins. It acts in cellular stress responses aiding cells in coping with conditions like heat or oxidative stress. Researchers recognize its important role in maintaining cellular protein homeostasis.

Biological function summary

Heat shock protein 46 is integral in cellular protection during stress conditions. It participates in forming complexes with other heat shock proteins enhancing its function in assisting proteins during synthesis and repair. This protein acts in maintaining the stability and function of other proteins preserving cellular health under stress. Its involvement in cellular processes signifies its importance in both cellular functioning and stress response mechanisms.

Pathways

P46 plays a critical role in the heat shock response and protein quality control pathways. It interacts with proteins such as HSP70 and HSP90 which participate in similar pathways ensuring proper folding and stability of newly synthesized proteins. These pathways protect cells from damage during stress connecting p46 to broader physiological responses. Its presence in these pathways emphasizes its significance in cellular recovery and adaptation processes.

P46 connects to neurodegenerative diseases and cancer. In neurodegenerative diseases such as Alzheimer's its function becomes important due to the accumulation of misfolded proteins sharing pathways with proteins like HSP70 which also counteracts protein misfolding. In cancer the overexpression of p46 has been observed linking it to tumor progression where it may stabilize proteins required for tumor growth and survival. These connections highlight how alterations in p46 levels may contribute to disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by association with the TREX complex.

See full target information POLDIP3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of infectious diseases 218:825-834 PubMed29684201

2018

Dysregulation of Epstein-Barr Virus Infection in Hypomorphic ZAP70 Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Hoshino,Takehiro Takashima,Kenichi Yoshida,Akira Morimoto,Yuta Kawahara,Tzu-Wen Yeh,Tsubasa Okano,Motoi Yamashita,Noriko Mitsuiki,Kohsuke Imai,Takashi Sakatani,Atsuko Nakazawa,Yusuke Okuno,Yuichi Shiraishi,Kenichi Chiba,Hiroko Tanaka,Satoru Miyano,Seishi Ogawa,Seiji Kojima,Tomohiro Morio,Hirokazu Kanegane

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p46 antibody

Immunoprecipitation - Anti-p46 antibody (AB134556)

Western blot - Anti-p46 antibody (AB134556)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Primary Antibodies

Primary Antibodies

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