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AB127564

Anti-P4HA1 antibody

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(1 Publication)

Rabbit Polyclonal P4HA1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA1 aa 300 to C-terminus.

View Alternative Names

P4HA, P4HA1, Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1

2 Images
Western blot - Anti-P4HA1 antibody (AB127564)
  • WB

Unknown

Western blot - Anti-P4HA1 antibody (AB127564)

7.5% SDS PAGE

All lanes:

Western blot - Anti-P4HA1 antibody (ab127564) at 1/1000 dilution

All lanes:

PC3 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB127564)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB127564)

ab127564 at 1/500 dilution staining P4HA1 in paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human P4HA1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Alternatively use Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

Store undiluted.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein P4HA1 also known as Prolyl 4-Hydroxylase Subunit Alpha 1 functions mechanically by catalyzing the hydroxylation of proline residues in collagen. This activity requires iron and ascorbate as cofactors. P4HA1 is a part of the prolyl 4-hydroxylase enzyme complex and contains a molecular mass of approximately 63 kDa. It expresses widely in various human tissues with high expression in the liver skin and vascular tissues reflecting its role in collagen synthesis.
Biological function summary

P4HA1 plays a central role in collagen biosynthesis and maturation by introducing hydroxyl groups into proline residues which stabilize the collagen triple helix. This protein functions as a critical component of the prolyl 4-hydroxylase complex which includes a beta subunit. Proper formation and stability of collagen fibers depend on P4HA1 activity impacting the structural integrity of connective tissues throughout the body.

Pathways

P4HA1's function integrates into the pathways of collagen biosynthesis and modification. It is significant in the extracellular matrix organization pathway which upholds the structural support and cellular signaling. Additionally P4HA1 interacts with other proteins like COL1A1 and PLOD1 during these processes assisting in the proper formation of extracellular matrix structures essential for tissue function and injury repair.

P4HA1 has a strong association with fibrotic diseases such as liver fibrosis and pulmonary fibrosis where excessive collagen deposition is a hallmark. Dysregulation of P4HA1 may also play a role in tumor progression in cancers by promoting extracellular matrix remodeling therefore facilitating tumor invasion and metastasis. The protein interacts with pathways involving vascular endothelial growth factor (VEGF) which drives angiogenesis and is implicated in these disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
See full target information P4HA1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 21:145 PubMed33552264

2021

P4HA1 regulates human colorectal cancer cells through HIF1α-mediated Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Zhang,Yue Yin,Hongye Zhao,Yan Shi,Wei Zhang,Zhengpeng Yang,Tingting Liu,Yonghong Huang,Zhanjiang Yu
View all publications
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Product promise

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