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AB244400

Anti-P4HA1 antibody

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(5 Publications)

Rabbit Polyclonal P4HA1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA1 aa 150-350.

View Alternative Names

P4HA, P4HA1, Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-P4HA1 antibody (AB244400)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-P4HA1 antibody (AB244400)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P4HA1 (green) using ab244400 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)

Paraffin-embedded human testis tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)

Paraffin-embedded human placenta tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)

Paraffin-embedded human endometrium tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)

Paraffin-embedded human cerebellum tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (AB244400)

Paraffin-embedded human pancreas tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.

Western blot - Anti-P4HA1 antibody (AB244400)
  • WB

Unknown

Western blot - Anti-P4HA1 antibody (AB244400)

All lanes:

Western blot - Anti-P4HA1 antibody (ab244400) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Predicted band size: 61 kDa

false

Western blot - Anti-P4HA1 antibody (AB244400)
  • WB

Unknown

Western blot - Anti-P4HA1 antibody (AB244400)

All lanes:

Western blot - Anti-P4HA1 antibody (ab244400) at 0.4 µg/mL

Lane 1:

PC-3 (Human prostate adenocarcinoma cell line) cell lysate

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human P4HA1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P13674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein P4HA1 also known as Prolyl 4-Hydroxylase Subunit Alpha 1 functions mechanically by catalyzing the hydroxylation of proline residues in collagen. This activity requires iron and ascorbate as cofactors. P4HA1 is a part of the prolyl 4-hydroxylase enzyme complex and contains a molecular mass of approximately 63 kDa. It expresses widely in various human tissues with high expression in the liver skin and vascular tissues reflecting its role in collagen synthesis.
Biological function summary

P4HA1 plays a central role in collagen biosynthesis and maturation by introducing hydroxyl groups into proline residues which stabilize the collagen triple helix. This protein functions as a critical component of the prolyl 4-hydroxylase complex which includes a beta subunit. Proper formation and stability of collagen fibers depend on P4HA1 activity impacting the structural integrity of connective tissues throughout the body.

Pathways

P4HA1's function integrates into the pathways of collagen biosynthesis and modification. It is significant in the extracellular matrix organization pathway which upholds the structural support and cellular signaling. Additionally P4HA1 interacts with other proteins like COL1A1 and PLOD1 during these processes assisting in the proper formation of extracellular matrix structures essential for tissue function and injury repair.

P4HA1 has a strong association with fibrotic diseases such as liver fibrosis and pulmonary fibrosis where excessive collagen deposition is a hallmark. Dysregulation of P4HA1 may also play a role in tumor progression in cancers by promoting extracellular matrix remodeling therefore facilitating tumor invasion and metastasis. The protein interacts with pathways involving vascular endothelial growth factor (VEGF) which drives angiogenesis and is implicated in these disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
See full target information P4HA1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 22:19-29 PubMed36582605

2022

Cancer stem cell-like cells-derived exosomal lncRNA promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Wu,Yonggang He,Xin Liu,Fangxiu Luo,Yimei Jiang,Ming Xiang,Ren Zhao

Cancer science 114:490-503 PubMed36227125

2022

circTOP2A functions as a ceRNA to promote glioma progression by upregulating RPN2.

Applications

Unspecified application

Species

Unspecified reactive species

Jikui Sun,Jinhuan Wang,Meng Li,Shengjie Li,Hanyun Li,Yan Lu,Feng Li,Tao Xin,Feng Jin

Frontiers in genetics 13:848456 PubMed35812752

2022

The Role of P4HA1 in Multiple Cancer Types and its Potential as a Target in Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Yu-Zheng Ge,Yiguan Qian,Ke Chen,Feng Zhao,Zhiqiang Qin,Liuhua Zhou,Luwei Xu,Zheng Xu,Quanliang Dou,Ruipeng Jia

The Journal of experimental medicine 219: PubMed35389431

2022

IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Chen,Junjie Zhao,Tomasz Herjan,Lingzi Hong,Yun Liao,Caini Liu,Kommireddy Vasu,Han Wang,Austin Thompson,Paul L Fox,Brian R Gastman,Xiao Li,Xiaoxia Li

Biochemical and biophysical research communication 516:606-612 PubMed31239153

2019

P4HA1/HIF1α feedback loop drives the glycolytic and malignant phenotypes of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

X P Cao,Y Cao,W J Li,H H Zhang,Z M Zhu
View all publications
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