AB59497

Anti-P4HA1 antibody

Name: Anti-P4HA1 antibody (ab59497) | Abcam
Brand: Abcam Limited
SKU: AB59497
Price: 545 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(8 Publications)

Goat Polyclonal P4HA1 antibody. Suitable for ICC and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human P4HA1 aa 400-450.

View Alternative Names

P4HA, P4HA1, Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1

1 Images

Supplier Data

Immunocytochemistry - Anti-P4HA1 antibody (AB59497)

Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling P4HA1 with ab59497 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor^® 488 secondary antibody at 2 μg/mL. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG at 10 μg/mL followed by Alexa Fluor^® 488 secondary antibody at 2 μg/ml.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human P4HA1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

P13674

Specificity

This antibody is expected to recognise both reported isoforms.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein P4HA1 also known as Prolyl 4-Hydroxylase Subunit Alpha 1 functions mechanically by catalyzing the hydroxylation of proline residues in collagen. This activity requires iron and ascorbate as cofactors. P4HA1 is a part of the prolyl 4-hydroxylase enzyme complex and contains a molecular mass of approximately 63 kDa. It expresses widely in various human tissues with high expression in the liver skin and vascular tissues reflecting its role in collagen synthesis.

Biological function summary

P4HA1 plays a central role in collagen biosynthesis and maturation by introducing hydroxyl groups into proline residues which stabilize the collagen triple helix. This protein functions as a critical component of the prolyl 4-hydroxylase complex which includes a beta subunit. Proper formation and stability of collagen fibers depend on P4HA1 activity impacting the structural integrity of connective tissues throughout the body.

Pathways

P4HA1's function integrates into the pathways of collagen biosynthesis and modification. It is significant in the extracellular matrix organization pathway which upholds the structural support and cellular signaling. Additionally P4HA1 interacts with other proteins like COL1A1 and PLOD1 during these processes assisting in the proper formation of extracellular matrix structures essential for tissue function and injury repair.

P4HA1 has a strong association with fibrotic diseases such as liver fibrosis and pulmonary fibrosis where excessive collagen deposition is a hallmark. Dysregulation of P4HA1 may also play a role in tumor progression in cancers by promoting extracellular matrix remodeling therefore facilitating tumor invasion and metastasis. The protein interacts with pathways involving vascular endothelial growth factor (VEGF) which drives angiogenesis and is implicated in these disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

See full target information P4HA1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:16079 PubMed37752218

2023

The role of vesicle trafficking genes in osteoblast differentiation and function.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhu,Yingying Su,Jamie Wang,Joy Y Wu

Journal of cellular and molecular medicine 23:7449-7461 PubMed31512366

2019

Silencing of NONO inhibits abdominal aortic aneurysm in apolipoprotein E-knockout mice via collagen deposition and inflammatory inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xingli Xu,Fang Zhang,Yue Lu,Sufang Yu,Wenqian Sun,Shangwen Sun,Jing Cheng,Jing Ma,Meng Zhang,Cheng Zhang,Yun Zhang,Kai Zhang

Biomedicine & pharmacotherapy = Biomedecine & phar 118:109224 PubMed31349139

2019

Liraglutide protects high-glucose-stimulated fibroblasts by activating the CD36-JNK-AP1 pathway to downregulate P4HA1.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Zhao,Huiqiang Chen,Chao Cheng,Juan Zhang,Zhi Yan,Jiangying Kuang,Feng Kong,Chunyan Li,Qinghua Lu

Acta biochimica et biophysica Sinica 51:293-300 PubMed30883649

2019

Liraglutide alleviates cardiac fibrosis through inhibiting P4hα-1 expression in STZ-induced diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Zhao,Huiqiang Chen,Fei Xu,Juan Wang,Yusheng Liu,Xiaowei Xing,Linlin Guo,Mingxiang Zhang,Qinghua Lu

Journal of cellular physiology 234:6561-6581 PubMed30417486

2018

Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen-induced acute liver injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Feng,Ningwei Zhao,Wenkai Xia,ChengJie Liang,Guoxin Dai,Jian Yang,Jingxia Sun,Lanying Liu,Lan Luo,Jie Yang

American journal of physiology. Heart and circulat 315:H1269-H1278 PubMed30141979

2018

Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Daniella Corporan,Daisuke Onohara,Roberto Hernandez-Merlo,Alicja Sielicka,Muralidhar Padala

PloS one 11:e0153919 PubMed27120457

2016

Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-κB Pathway.

Applications

Species

Human

Xiao-Lin Wang,Yuan-Li Zhou,Wei Sun,Li Li

PloS one 9:e100770 PubMed24950301

2014

Prostatic inflammation induces fibrosis in a mouse model of chronic bacterial infection.

Applications

IHC-P

Species

Mouse

Letitia Wong,Paul R Hutson,Wade Bushman

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-P4HA1 antibody

Immunocytochemistry - Anti-P4HA1 antibody (AB59497)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise