AB233197

Anti-P4HA2 antibody

Name: Anti-P4HA2 antibody (ab233197) | Abcam
Brand: Abcam Limited
SKU: AB233197
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal P4HA2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA2 aa 200 to C-terminus.

View Alternative Names

UNQ290/PRO330, P4HA2, Prolyl 4-hydroxylase subunit alpha-2, 4-PH alpha-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody (AB233197)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody (AB233197)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for P4HA2 using ab233197 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-P4HA2 antibody (AB233197)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-P4HA2 antibody (AB233197)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HA2 using ab233197 at 2 μg/ml in ICC/IF. FITC staining.

Supplier Data

Western blot - Anti-P4HA2 antibody (AB233197)

All lanes:

Western blot - Anti-P4HA2 antibody (ab233197) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-P4HA2 antibody (AB233197)

All lanes:

Western blot - Anti-P4HA2 antibody (ab233197) at 2 µg/mL

All lanes:

Recombinant human P4HA2 protein

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human P4HA2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15460

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P4HA2 also known as Prolyl 4-hydroxylase subunit alpha-2 is an enzyme with an approximate mass of 60 kDa. It plays a role in catalyzing the hydroxylation of proline residues in collagen strands which is essential for the proper formation of the collagen triple helix structure. P4HA2 can be found most abundantly in tissues that require strong extracellular matrices such as skin tendons and cartilage. Its expression in these tissues suggests a critical role in maintaining structural integrity.

Biological function summary

Prolyl 4-hydroxylase functions within a larger enzyme complex by working alongside other subunits to ensure efficient collagen biosynthesis. This complex modifies proline residues on collagen peptides ensuring structural stability and mechanical strength of the extracellular matrix. By contributing to collagen stability P4HA2 supports cellular functions related to tissue maintenance and repair. Its activity is important in physiological environments where collagen is abundant emphasizing its role in vertebrate development and organism physiology.

Pathways

Collagen biosynthesis and modification serve as significant pathways involving P4HA2. This enzyme contributes to pathways related to the post-translational modification of collagen which is essential for extracellular matrix organization and cellular function. P4HA2 interacts with proteins such as collagen type I and type III playing an important role in the maturation of collagen fibers and influencing tissue resilience and elasticity. Such interactions highlight its involvement in fundamental biological processes that sustain organismal structure and function.

P4HA2 has associations with fibrosis and certain cancers. During fibrotic progression dysregulation of collagen production often involves elevated P4HA2 levels driving excessive extracellular matrix deposition and tissue stiffening. This condition can connect P4HA2 to fibrosis-related proteins such as TGF-beta which regulate extracellular matrix production. In cancer P4HA2 may contribute to tumor microenvironment changes influencing tumor growth and metastasis through interactions with proteins like MMPs (matrix metalloproteinases). Understanding these connections not only helps in identifying potential therapeutic targets but also in elucidating the pathophysiology of collagen-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

See full target information P4HA2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Medical oncology (Northwood, London, England) 41:163 PubMed38777998

2024

P4HA2 contributes to head and neck squamous cell carcinoma progression and EMT through PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ling Wu,Wan Liu,Tingting Zhao,Jing Jin

Nature 622:173-179 PubMed37731000

2023

Acetyl-methyllysine marks chromatin at active transcription start sites.

Applications

Unspecified application

Species

Unspecified reactive species

William J Lu-Culligan,Leah J Connor,Yixuan Xie,Babatunde E Ekundayo,Brendan T Rose,Martin Machyna,Andreas P Pintado-Urbanc,Joshua T Zimmer,Isaac W Vock,Natarajan V Bhanu,Megan C King,Benjamin A Garcia,Franziska Bleichert,Matthew D Simon

Journal of oncology 2021:1406853 PubMed34434233

2021

P4HA2 Promotes Epithelial-to-Mesenchymal Transition and Glioma Malignancy through the Collagen-Dependent PI3K/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Lin,Lei Jiang,Xiaogang Wang,Wenxin Wei,Chaoli Song,Yong Cui,Xiaojun Wu,GuanZhong Qiu

Journal of cellular and molecular medicine 25:448-462 PubMed33215860

2020

Comprehensive analysis of partial epithelial mesenchymal transition-related genes in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Lei,Wei Yan,Zhuoying Lin,Jingmei Liu,Dean Tian,Ping Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P4HA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody (AB233197)

Immunocytochemistry/ Immunofluorescence - Anti-P4HA2 antibody (AB233197)

Western blot - Anti-P4HA2 antibody (AB233197)

Western blot - Anti-P4HA2 antibody (AB233197)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise