AB70887

Anti-P4HA2 antibody

Name: Anti-P4HA2 antibody (ab70887) | Abcam
Brand: Abcam Limited
SKU: AB70887
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal P4HA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human P4HA2.

View Alternative Names

UNQ290/PRO330, P4HA2, Prolyl 4-hydroxylase subunit alpha-2, 4-PH alpha-2

3 Images

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Immunoprecipitation - Anti-P4HA2 antibody (AB70887)

HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
Lane 2 : control IgG.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-P4HA2 antibody (ab70887)

Predicted band size: 61 kDa

false

Unknown

Western blot - Anti-P4HA2 antibody (AB70887)

All lanes:

Western blot - Anti-P4HA2 antibody (ab70887) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 61 kDa

Observed band size: 28 kDa,33 kDa,50 kDa,61 kDa

true

Exposure time: 3min

AbReview28331****

Western blot - Anti-P4HA2 antibody (AB70887)

Good immunoreactivity observed in RIE-1 cell lysate that is consistent with the mobility of full-length P4HA2. A faster migrating species of ~35 kDa has been commonly observed with other antibodies (including this one) that could be a degradation product or alternatively spliced form of P4HA2. Further charcaterisation is required.

All lanes:

Western blot - Anti-P4HA2 antibody (ab70887) at 1 µg/mL

Lane 1:

Whole cell lysate prepared from 9L cells at 20 µg

Lane 2:

Whole cell lysate prepared from PC12 cells at 20 µg

Lane 3:

Whole cell lysate prepared from RIE-1 cells at 20 µg

Secondary

All lanes:

HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution

Predicted band size: 61 kDa

Observed band size: 35 kDa,61 kDa

true

Exposure time: 5min

Image courtesy of an anonymous Abreview.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human P4HA2. The exact immunogen used to generate this antibody is proprietary information.

O15460

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Baboon": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P4HA2 also known as Prolyl 4-hydroxylase subunit alpha-2 is an enzyme with an approximate mass of 60 kDa. It plays a role in catalyzing the hydroxylation of proline residues in collagen strands which is essential for the proper formation of the collagen triple helix structure. P4HA2 can be found most abundantly in tissues that require strong extracellular matrices such as skin tendons and cartilage. Its expression in these tissues suggests a critical role in maintaining structural integrity.

Biological function summary

Prolyl 4-hydroxylase functions within a larger enzyme complex by working alongside other subunits to ensure efficient collagen biosynthesis. This complex modifies proline residues on collagen peptides ensuring structural stability and mechanical strength of the extracellular matrix. By contributing to collagen stability P4HA2 supports cellular functions related to tissue maintenance and repair. Its activity is important in physiological environments where collagen is abundant emphasizing its role in vertebrate development and organism physiology.

Pathways

Collagen biosynthesis and modification serve as significant pathways involving P4HA2. This enzyme contributes to pathways related to the post-translational modification of collagen which is essential for extracellular matrix organization and cellular function. P4HA2 interacts with proteins such as collagen type I and type III playing an important role in the maturation of collagen fibers and influencing tissue resilience and elasticity. Such interactions highlight its involvement in fundamental biological processes that sustain organismal structure and function.

P4HA2 has associations with fibrosis and certain cancers. During fibrotic progression dysregulation of collagen production often involves elevated P4HA2 levels driving excessive extracellular matrix deposition and tissue stiffening. This condition can connect P4HA2 to fibrosis-related proteins such as TGF-beta which regulate extracellular matrix production. In cancer P4HA2 may contribute to tumor microenvironment changes influencing tumor growth and metastasis through interactions with proteins like MMPs (matrix metalloproteinases). Understanding these connections not only helps in identifying potential therapeutic targets but also in elucidating the pathophysiology of collagen-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

See full target information P4HA2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1581860 PubMed40406250

2025

Integrated analysis identifies P4HA2 as a key regulator of STAT1-mediated colorectal cancer progression and a potential biomarker for precision therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Qianshi Zhang,Yinan Zhang,Zhiwei Sun,Huanle Wang,Guohang Dai,Yue Meng,Shasha Shi,Shuangyi Ren

The EMBO journal 38:e100532 PubMed31268606

2019

RASSF1A controls tissue stiffness and cancer stem-like cells in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Pankova,Yanyan Jiang,Maria Chatzifrangkeskou,Iolanda Vendrell,Jon Buzzelli,Anderson Ryan,Cameron Brown,Eric O'Neill

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P4HA2 antibody

Immunoprecipitation - Anti-P4HA2 antibody (AB70887)

Western blot - Anti-P4HA2 antibody (AB70887)

Western blot - Anti-P4HA2 antibody (AB70887)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise