Rabbit Polyclonal P4HA3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA3 aa 100-400.
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Publications
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- Frontiers in cardiovascular medicine 8:6093002021Profiling of miR-205/P4HA3 Following Angiotensin II-Induced Atrial Fibrosis: Implications for Atrial Fibrillation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZezhou Xiao,Desai Pavan Kumar Reddy,Chuqing Xue,Ximao Liu,Xiong Chen,Jiale Li,Xiao Ling,Shaoyi ZhengPubMed 33981730
- Breast cancer research : BCR 22:642020Age-associated genes in human mammary gland drive human breast cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Gu et. al.PubMed 32539762
- Cell death & disease 11:1362020Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206 M2-like macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Wang et. al.PubMed 32075954
- Aging 11:8845-88592019COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiqing Long et. al.PubMed 31627190
- Technology in cancer research & treatment 17:15330338187964852018P4HA3 is Epigenetically Activated by Slug in Gastric Cancer and its Deregulation is Associated With Enhanced Metastasis and Poor Survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHu Song,Lingling Liu,Zhaoquan Song,Yongqiang Ren,Chao Li,Jiege HuoPubMed 30198421
- Nature communications 8:160312017Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions.Applications:WB, IHCReactive species:Unspecified reactive speciesOrianne Olivares et. al.PubMed 28685754
- Fibrogenesis & tissue repair 6:152013Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences.Applications:IHC-PReactive species:Unspecified reactive speciesTaisuke Akamatsu et. al.PubMed 23927729
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human P4HA3 aa 100-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z4N8
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Alternative names
UNQ711/PRO1374, P4HA3, Prolyl 4-hydroxylase subunit alpha-3, 4-PH alpha-3
Recommended products
Rabbit Polyclonal P4HA3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA3 aa 100-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human P4HA3 aa 100-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z4N8
- Purification technique
- Affinity purification Protein A
- Reconstitution
- Reconstitute in 200 µL of water
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein P4HA3 also known as Prolyl 4-Hydroxylase Subunit Alpha 3 functions as an enzyme involved in collagen synthesis. It is part of the prolyl 4-hydroxylase complex which catalyzes the hydroxylation of proline residues in collagen aiding its stability and structural integrity. P4HA3 has a molecular mass of approximately 61 kDa. Expression of this protein occurs widely especially in connective tissues where collagen is a major component.
- Biological function summary
P4HA3 plays an important role in remodeling and maintaining the extracellular matrix through its contribution to collagen biosynthesis. As a member of the prolyl 4-hydroxylase complex it partners with beta subunits to form a functional enzyme. This association is critical for hydroxyproline formation which is important for assembling stable collagen triple helices. The enzyme's activity impacts cellular processes such as migration adhesion and growth embedded in tissue repair and development.
- Pathways
P4HA3 is an integral part of the collagen biosynthesis pathway. This pathway includes the post-translational modification of collagen proteins which is essential for producing functional collagen fibrils. It works in concert with other proteins like P4HA1 and P4HB which are also involved in collagen hydroxylation. The activity of P4HA3 impacts the TGF-beta signaling pathway which influences various cellular functions including proliferation and differentiation.
- Associated diseases and disorders
Alterations in P4HA3 are linked to fibrosis and cancer. Fibrosis involves excessive collagen deposition often driven by increased activity of P4HA3 leading to tissue scarring and functional impairment as observed in liver and lung conditions. In cancer upregulation of P4HA3 can promote tumor proliferation and metastasis changing the tumor microenvironment to favor cancer cell survival. P4HA3’s interaction with P4HB can enhance these pathological processes highlighting its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-P4HA3 antibody (ab101657)
All lanes: Western blot - Anti-P4HA3 antibody (ab101657) at 1/500 dilution
Lane 1: Human fetal kidney lysate
Lane 2: Human fetal liver lysate
Predicted band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA3 antibody (ab101657)
ab101657 at 1/100 dilution staining P4HA3 in Human fetal liver by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
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