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AB101657

Anti-P4HA3 antibody

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(8 Publications)

Rabbit Polyclonal P4HA3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA3 aa 100-400.

View Alternative Names

UNQ711/PRO1374, P4HA3, Prolyl 4-hydroxylase subunit alpha-3, 4-PH alpha-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA3 antibody (AB101657)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA3 antibody (AB101657)

ab101657 at 1/100 dilution staining P4HA3 in Human fetal liver by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Western blot - Anti-P4HA3 antibody (AB101657)
  • WB

Unknown

Western blot - Anti-P4HA3 antibody (AB101657)

All lanes:

Western blot - Anti-P4HA3 antibody (ab101657) at 1/500 dilution

Lane 1:

Human fetal kidney lysate

Lane 2:

Human fetal liver lysate

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human P4HA3 aa 100-400. The exact immunogen used to generate this antibody is proprietary information.

Q7Z4N8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein P4HA3 also known as Prolyl 4-Hydroxylase Subunit Alpha 3 functions as an enzyme involved in collagen synthesis. It is part of the prolyl 4-hydroxylase complex which catalyzes the hydroxylation of proline residues in collagen aiding its stability and structural integrity. P4HA3 has a molecular mass of approximately 61 kDa. Expression of this protein occurs widely especially in connective tissues where collagen is a major component.
Biological function summary

P4HA3 plays an important role in remodeling and maintaining the extracellular matrix through its contribution to collagen biosynthesis. As a member of the prolyl 4-hydroxylase complex it partners with beta subunits to form a functional enzyme. This association is critical for hydroxyproline formation which is important for assembling stable collagen triple helices. The enzyme's activity impacts cellular processes such as migration adhesion and growth embedded in tissue repair and development.

Pathways

P4HA3 is an integral part of the collagen biosynthesis pathway. This pathway includes the post-translational modification of collagen proteins which is essential for producing functional collagen fibrils. It works in concert with other proteins like P4HA1 and P4HB which are also involved in collagen hydroxylation. The activity of P4HA3 impacts the TGF-beta signaling pathway which influences various cellular functions including proliferation and differentiation.

Alterations in P4HA3 are linked to fibrosis and cancer. Fibrosis involves excessive collagen deposition often driven by increased activity of P4HA3 leading to tissue scarring and functional impairment as observed in liver and lung conditions. In cancer upregulation of P4HA3 can promote tumor proliferation and metastasis changing the tumor microenvironment to favor cancer cell survival. P4HA3’s interaction with P4HB can enhance these pathological processes highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
See full target information P4HA3
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 56:1833-1847 PubMed39394821

2024

Collagen prolyl 4-hydroxylase subunit α member-induced head and neck squamous cell carcinoma aggressiveness is antagonized by LLGL2 via reduced expression of occludin.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Xu,Run Shi,Jie Yang,Heng Chen,Shihua Liu,Shupei Yu,Sasa Li,Wenqiang He,Man-Sun Sy,Mingjian Lu,Huixia Zhang,Chaoyang Li

Frontiers in cardiovascular medicine 8:609300 PubMed33981730

2021

Profiling of miR-205/P4HA3 Following Angiotensin II-Induced Atrial Fibrosis: Implications for Atrial Fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Zezhou Xiao,Desai Pavan Kumar Reddy,Chuqing Xue,Ximao Liu,Xiong Chen,Jiale Li,Xiao Ling,Shaoyi Zheng

Breast cancer research : BCR 22:64 PubMed32539762

2020

Age-associated genes in human mammary gland drive human breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Gu,Bingzhi Wang,Haiyan Zhu,You Zhou,Aaron M Horning,Tim H-M Huang,Yidong Chen,Peter Houghton,Zhao Lai,Joel E Michalek,Lu-Zhe Sun

Cell death & disease 11:136 PubMed32075954

2020

Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206 M2-like macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wang,Lizhi Xu,Zou Xiang,Yan Ren,Xiufen Zheng,Qingya Zhao,Qunzhi Zhou,Yuefen Zhou,Lin Xu,Yaping Wang

Aging 11:8845-8859 PubMed31627190

2019

COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqing Long,Zhuohui Liu,Jinghui Li,Hualin Yu

Technology in cancer research & treatment 17:1533033818796485 PubMed30198421

2018

P4HA3 is Epigenetically Activated by Slug in Gastric Cancer and its Deregulation is Associated With Enhanced Metastasis and Poor Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Song,Lingling Liu,Zhaoquan Song,Yongqiang Ren,Chao Li,Jiege Huo

Nature communications 8:16031 PubMed28685754

2017

Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Orianne Olivares,Jared R Mayers,Victoire Gouirand,Margaret E Torrence,Tristan Gicquel,Laurence Borge,Sophie Lac,Julie Roques,Marie-Noëlle Lavaut,Patrice Berthezène,Marion Rubis,Veronique Secq,Stéphane Garcia,Vincent Moutardier,Dominique Lombardo,Juan Lucio Iovanna,Richard Tomasini,Fabienne Guillaumond,Matthew G Vander Heiden,Sophie Vasseur

Fibrogenesis & tissue repair 6:15 PubMed23927729

2013

Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences.

Applications

IHC-P

Species

Unspecified reactive species

Taisuke Akamatsu,Yosifumi Arai,Isao Kosugi,Hideya Kawasaki,Shiori Meguro,Makiko Sakao,Kiyoshi Shibata,Takafumi Suda,Kingo Chida,Toshihide Iwashita
View all publications
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Product promise

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