AB109752

Anti-p50 dynamitin antibody [EPR5094]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal p50 dynamitin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

DCTN50, DCTN2, Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, p50 dynamitin, DCTN-50

2 Images

Flow Cytometry (Intracellular) - Anti-p50 dynamitin antibody [EPR5094] (AB109752)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-p50 dynamitin antibody [EPR5094] (AB109752)

ab109752 at 1/10 dilution staining p50 dynamitin in Saos2 cells by intracellular flow cytometry (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-p50 dynamitin antibody [EPR5094] (AB109752)

All lanes:

Western blot - Anti-p50 dynamitin antibody [EPR5094] (ab109752) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

Saos2 cell lysate at 10 µg

Lane 4:

SH SY5Y cell lysate at 10 µg

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5094

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P50 dynamitin also known as DCTN2 or dynactin subunit 2 is a protein component of the dynactin complex with a molecular mass of approximately 50 kDa. This protein is expressed in various tissues where it plays an important role in intracellular transport mechanisms. p50 dynamitin is essential for the proper functioning of the dynein-dynactin motor protein complex which is important for the transport of organelles and vesicles along microtubules within cells.

Biological function summary

P50 dynamitin serves as a regulator within the dynactin complex ensuring its structural integrity and facilitating interaction with dynein motor proteins. It is part of the larger dynactin complex which consists of over 20 subunits. p50 dynamitin's role in this complex directly impacts cytoplasmic and nuclear activities including the trafficking of endosomes lysosomes and other cargo within cells. The proper functioning of this complex is necessary for maintaining the correct intracellular transport which affects various cellular processes.

Pathways

P50 dynamitin is involved in the cytoskeletal network through its interaction with the dynein motor protein pathway and is closely related to microtubule dynamics. Dynactin of which p50 is a part functions with dynein to mediate minus-end-directed movement along microtubules necessary for processes such as mitotic spindle formation and chromosome alignment. This relationship with dynein highlights its role in cell division and intracellular transport impacting neurological pathway regulation.

P50 dynamitin has been linked to neurological disorders like motor neuron disease and amyotrophic lateral sclerosis (ALS). The dysfunction of the dynactin-dynein complex where p50 dynamitin plays a regulatory role can lead to disrupted neuronal transport processes contributing to these diseases. Additionally its association with the protein TDP-43 points to a connection with neurodegenerative conditions further illustrating its clinical significance in the pathology of ALS and related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In the dynactin soulder domain, binds the ACTR1A filament and acts as a molecular ruler to determine the length (By similarity). Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development (By similarity).

See full target information DCTN2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 20:229 PubMed33149784

2020

Ginseng polysaccharide inhibits MDA-MB-231 cell proliferation by activating the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Haoliang Zhou,Yuxiang Yan,Xianbo Zhang,Ting Zhao,Jiangang Xu,Ruokuo Han

Journal of virology 90:6896-6905 PubMed27194765

2016

Dynein Regulators Are Important for Ecotropic Murine Leukemia Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Roger Valle-Tenney,Tatiana Opazo,Jorge Cancino,Stephen P Goff,Gloria Arriagada

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p50 dynamitin antibody [EPR5094]

Flow Cytometry (Intracellular) - Anti-p50 dynamitin antibody [EPR5094] (AB109752)

Western blot - Anti-p50 dynamitin antibody [EPR5094] (AB109752)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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