AB248521

Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal p50 dynamitin antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DCTN50, DCTN2, Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, p50 dynamitin, DCTN-50

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

This data was developed using ab133492, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of p50 dynamitin in SH-SY5Y cells, using ab133492 at a dilution of 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling p50 dynamitin with ab133492 at 1/400 dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab133492 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. This data was developed using ab133492, the same antibody clone in a different buffer formulation.

Unknown

Western blot - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

This data was developed using ab133492, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-p50 dynamitin antibody [EPR5095] (<a href='/en-us/products/primary-antibodies/p50-dynamitin-antibody-epr5095-ab133492'>ab133492</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

Saos2 cell lysate at 10 µg

Lane 4:

SH SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5095

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab248521 is the carrier-free version of ab133492.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P50 dynamitin also known as DCTN2 or dynactin subunit 2 is a protein component of the dynactin complex with a molecular mass of approximately 50 kDa. This protein is expressed in various tissues where it plays an important role in intracellular transport mechanisms. p50 dynamitin is essential for the proper functioning of the dynein-dynactin motor protein complex which is important for the transport of organelles and vesicles along microtubules within cells.

Biological function summary

P50 dynamitin serves as a regulator within the dynactin complex ensuring its structural integrity and facilitating interaction with dynein motor proteins. It is part of the larger dynactin complex which consists of over 20 subunits. p50 dynamitin's role in this complex directly impacts cytoplasmic and nuclear activities including the trafficking of endosomes lysosomes and other cargo within cells. The proper functioning of this complex is necessary for maintaining the correct intracellular transport which affects various cellular processes.

Pathways

P50 dynamitin is involved in the cytoskeletal network through its interaction with the dynein motor protein pathway and is closely related to microtubule dynamics. Dynactin of which p50 is a part functions with dynein to mediate minus-end-directed movement along microtubules necessary for processes such as mitotic spindle formation and chromosome alignment. This relationship with dynein highlights its role in cell division and intracellular transport impacting neurological pathway regulation.

P50 dynamitin has been linked to neurological disorders like motor neuron disease and amyotrophic lateral sclerosis (ALS). The dysfunction of the dynactin-dynein complex where p50 dynamitin plays a regulatory role can lead to disrupted neuronal transport processes contributing to these diseases. Additionally its association with the protein TDP-43 points to a connection with neurodegenerative conditions further illustrating its clinical significance in the pathology of ALS and related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In the dynactin soulder domain, binds the ACTR1A filament and acts as a molecular ruler to determine the length (By similarity). Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development (By similarity).

See full target information DCTN2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Psychiatry investigation 22:699-713 PubMed40566894

2025

Transcriptional Landscape and Biomarker Discovery for Endoplasmic Reticulum Stress in Alzheimer's Disease: An Ex Vivo Study Using Patients-Derived Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yeojin Kim,You Jin Nam,Sunwoo Yoon,Young Joon Cho,Ho Min Song,Seongmin Kim,Donghyuk Shin,Jin Young Noh,Sun Min Lee,So Young Moon,Eun-Joo Kim,Soo Hyun Cho,Byeong C Kim,Seong Hye Choi,Sang Won Seo,Jin Wook Choi,Young-Sil An,Bumhee Park,Young Joon Park,Hee Young Kang,Hyun Goo Woo,Yong Hyuk Cho,Sunhwa Hong,Sang Joon Son,Sang-Rae Lee,Chang Hyung Hong,Hyun Woong Roh

BioMed research international 2022:9940566 PubMed35127947

2022

Prognostic Bone Metastasis-Associated Immune-Related Genes Regulated by Transcription Factors in Mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiquan Hao,Siqiao Wang,Zixuan Zheng,Jiehan Li,Wanting Fu,Donglin Han,Yinrou Huang,Qing Lin,Shuyuan Xian,Penghui Yan,Man Li,Ruoyi Lin,Tong Meng,Jie Zhang,Zongqiang Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

Western blot - Anti-p50 dynamitin antibody [EPR5095] - BSA and Azide free (AB248521)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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