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Rabbit Recombinant Multiclonal P53 acetyl K305 + K370 + K373 antibody. Suitable for Dot, WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Synthetic peptide, Human, Mouse samples.

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Images

Western blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (AB321819), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (AB321819), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (AB321819), expandable thumbnail
  • Immunoprecipitation - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (AB321819), expandable thumbnail
  • Dot Blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (AB321819), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBICC/IFFlow Cyt (Intra)IPIHC-P
Human
Expected
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

-

Species

Mouse

Dilution info

1/2000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Human, Synthetic peptide, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Alternative names

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

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Rabbit Recombinant Multiclonal P53 acetyl K305 + K370 + K373 antibody. Suitable for Dot, WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Synthetic peptide, Human, Mouse samples.

Alternative names

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1200

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for human IP.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Activity summary

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

Associated diseases and disorders

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Western blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression of acetyl p53 is upregulated in response to doxorubicin and TSA treatment (PMID: 15123817).

    The identity of the lower MW band at approximately 15 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 treated with 0.5µM Doxorubicin (Doxorubicin hydrochloride, Topoisomerase II inhibitor ab120629) for 24 hours whole cell lysate at 20 µg

    Lane 3: NIH/3T3 treated with 0.5µM Doxorubicin and 500ng/ml TSA(Trichostatin A) for 24 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 53 kDa, 36 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Untreated HeLa (human cervical adenocarcinoma epithelial cell) (Black) / HeLa treated with 0.5µM Doxorubicin for 24h (Megenta) / HeLa treated with 0.5µM Doxorubicin and 500ng/mL Trichostatin A for 24h (Green) cells labelling p53 (acetyl K305 + K370 + K373) with ab321819 at 1/50 dilution (1ug) / Black, Megenta and Green compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Untreated NIH/3T3 (mouse embryonic fibroblast) (Black) / NIH/3T3 treated with 0.5µM Doxorubicin for 24h (Megenta) / NIH/3T3 treated with 0.5µM Doxorubicin and 500ng/mL Trichostatin A for 24h (Green) cells labelling p53 (acetyl K305 + K370 + K373) with ab321819 at 1/50 dilution (1ug) / Black, Megenta and Green compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Immunoprecipitation - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    p53 (acetyl K305 + K370 + K373) was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab321819 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab321819 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lane 2: ab321819 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab321819 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819) at 1/30 dilution

    All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 24s

  • Dot Blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Dot Blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    Dot blot analysis of p53 (acetyl K305 + K370 + K373) using ab321819 at 1:1000 (0.521 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Dot Blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819) at 1/1000 dilution

    Lane 1: Human p53 (acetyl K305) peptide

    Lanes 2, 4 and 6: Human p53 non-acetyl peptide

    Lane 3: Human p53 (acetyl K370) peptide

    Lane 5: Human p53 (acetyl K373) peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling p53 (acetyl K305 + K370 + K373) with ab321819 at 1/2000 (0.261 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing increased nuclear staining in NIH/3T3 cells (shown in green) treated with Doxorubicin (0.5 µM) for 24 h and further increased signal treated with Doxorubicin (0.5 µM) and Trichostatin A (500 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Western blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Western blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Saos-2 (PMID: 25490093)

    The expression of acetyl p53 is upregulated in response to doxorubicin and TSA treatment (PMID: 15123817).

    The identity of the lower MW band at approximately 15 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with 0.5µM Doxorubicin (Doxorubicin hydrochloride, Topoisomerase II inhibitor ab120629) for 24 hours whole cell lysate at 20 µg

    Lane 3: HeLa treated with 0.5µM Doxorubicin and 500ng/ml TSA(Trichostatin A) for 24 hours whole cell lysate at 20 µg

    Lane 4: Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 53 kDa, 36 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K305 + K370 + K373) antibody [RM1200] (ab321819)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling p53 (acetyl K305 + K370 + K373) with ab321819 at 1/2000 (0.261 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing increased nuclear staining in HeLa cells (shown in green) treated with Doxorubicin (0.5 µM) for 24 h and further increased signal treated with Doxorubicin (0.5 µM) and Trichostatin A (500 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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