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Rabbit Recombinant Monoclonal P53 acetyl K370 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 32 publications.


Images

Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544), expandable thumbnail
  • Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544), expandable thumbnail
  • Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Expected
Mouse
Expected
Tested
Tested
Tested
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Alternative names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Recommended products

Rabbit Recombinant Monoclonal P53 acetyl K370 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 32 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17496
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

Associated diseases and disorders

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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6 product images

  • Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544), expandable thumbnail

    Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

    p53 (acetyl K370) was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell extract treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours with ab183544 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab183544 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HepG2 whole cell extract treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours. Lane 2: PBS instead of HepG2
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

    Predicted band size: 43 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544), expandable thumbnail

    Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma) treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates at 10 µg

    Lane 2: HepG2 (human hepatocellular carcinoma) untreated whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

    Observed band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 NIH/3T3 (Mouse embyro fibroblast cells) cells labeling p53 (acetyl K370) with ab183544 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 IgG) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear and weakly cytoplasm staining on NIH/3T3 cell line.
    The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab183544 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (goat anti-rabbit Alexa Fluor®488 (IgG H&L) at 1/400 dilution.

    [J Cell Biol. May 22, 2006; 173(4): 533–544.]

  • Flow Cytometry (Intracellular) - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

    Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab183544 at 1/150 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544), expandable thumbnail

    Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544) at 1/10000 dilution

    Lane 1: NIH/3T3 (mouse embryo) treated with Trichostatin A 500 nM for 4hr whole cell lysates at 10 µg

    Lane 2: NIH/3T3 (mouse embryo) untreated whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544), expandable thumbnail

    Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544) at 1/1000 dilution

    Lane 1: C6 (rat glioma) treated with Trichostatin A 500 nM for 4hr whole cell lysates at 10 µg

    Lane 2: C6 (rat glioma) untreated whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa, 48 kDa

    Observed band size: 46 kDa, 53 kDa, 54 kDa

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