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AB183544

Anti-p53 (acetyl K370) antibody [EPR17496]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

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(1 Review)

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(40 Publications)

Rabbit Recombinant Monoclonal P53 acetyl K370 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 40 publications.

View Alternative Names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

6 Images
Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)
  • IP

Supplier Data

Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)

p53 (acetyl K370) was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell extract treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours with ab183544 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab183544 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : HepG2 whole cell extract treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours. Lane 2 : PBS instead of HepG2
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544)

Predicted band size: 43 kDa

Observed band size: 53 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 NIH/3T3 (Mouse embyro fibroblast cells) cells labeling p53 (acetyl K370) with ab183544 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear and weakly cytoplasm staining on NIH/3T3 cell line.
The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows;
1. ab183544 at 1/500 dilution followed by ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor®488 (IgG H&L) at 1/400 dilution.

[J Cell Biol. May 22, 2006; 173(4) : 533–544.]

Flow Cytometry (Intracellular) - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)

Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab183544 at 1/150 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)
  • WB

Supplier Data

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma) treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates at 10 µg

Lane 2:

HepG2 (human hepatocellular carcinoma) untreated whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)
  • WB

Supplier Data

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544) at 1/10000 dilution

Lane 1:

NIH/3T3 (mouse embryo) treated with Trichostatin A 500 nM for 4hr whole cell lysates at 10 µg

Lane 2:

NIH/3T3 (mouse embryo) untreated whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)
  • WB

Supplier Data

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-p53 (acetyl K370) antibody [EPR17496] (ab183544) at 1/1000 dilution

Lane 1:

C6 (rat glioma) treated with Trichostatin A 500 nM for 4hr whole cell lysates at 10 µg

Lane 2:

C6 (rat glioma) untreated whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa,48 kDa

Observed band size: 46 kDa,53 kDa,54 kDa

false

  • Carrier free

    Anti-p53 (acetyl K370) antibody [EPR17496] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-p53 (acetyl K370) antibody [EPR17496]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17496

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 35618207, PubMed : 36634798, PubMed : 38653238, PubMed : 9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17189187, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 38653238, PubMed : 9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed : 12524540, PubMed : 17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed : 12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed : 12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).
See full target information TP53 acetyl K370
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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:826 PubMed39543094

2024

FXR deficiency induced ferroptosis via modulation of the CBP-dependent p53 acetylation to suppress breast cancer growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Huang,Han Zhao,Hua Dai,Jinying Li,Xiafang Pan,Wentian Pan,Chunhua Xia,Fanglan Liu

Biomacromolecules 25:4215-4232 PubMed38845149

2024

Boron Neutron Capture Therapy Enhanced by Boronate Ester Polymer Micelles: Synthesis, Stability, and Tumor Inhibition Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Yun Fu,Yi-Lin Chiu,Shi-Chih Huang,Wei-Yuan Huang,Fang-Tzu Hsu,Han Yu Lee,Tzu-Wei Wang,Pei Yuin Keng

Journal of molecular and cellular cardiology 192:36-47 PubMed38734062

2024

Ferrostatin-1 specifically targets mitochondrial iron-sulfur clusters and aconitase to improve cardiac function in Sirtuin 3 cardiomyocyte knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aubrey C Cantrell,Jessie Besanson,Quinesha Williams,Ngoc Hoang,Kristin Edwards,G Reid Bishop,Yingjie Chen,Heng Zeng,Jian-Xiong Chen

Journal of neuroinflammation 20:208 PubMed37697347

2023

H3K18 lactylation of senescent microglia potentiates brain aging and Alzheimer's disease through the NFκB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wei,Xiaowen Yang,Jie Wang,Zhixiao Wang,Qiguang Wang,Yan Ding,Aiqing Yu

Nature communications 14:4820 PubMed37563109

2023

Hdac1 and Hdac2 regulate the quiescent state and survival of hair-follicle mesenchymal niche.

Applications

Unspecified application

Species

Unspecified reactive species

Hadas Sibony-Benyamini,Emil Aamar,David Enshell-Seijffers

Cell death discovery 9:12 PubMed36653338

2023

Nuclear SPHK2/S1P induces oxidative stress and NLRP3 inflammasome activation via promoting p53 acetylation in lipopolysaccharide-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Linjing Gong,Yue Shen,Sijiao Wang,Xinyuan Wang,Haiying Ji,Xu Wu,Lijuan Hu,Lei Zhu

Journal of cell science 135: PubMed36239052

2022

Δ133p53 coordinates ECM-driven morphogenesis and gene expression in three-dimensional mammary epithelial acini.

Applications

Unspecified application

Species

Unspecified reactive species

Sun-Young Lee,Claire Robertson,Alexandra Diot,Valerie Meuray,Jean-Christophe Bourdon,Mina J Bissell

Molecular medicine reports 26: PubMed36004485

2022

Oridonin attenuates dextran sulfate sodium‑induced ulcerative colitis in mice via the Sirt1/NF‑κB/p53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maonan Wang,Bo Xu,Lintao Liu,Dawei Wang

Skeletal muscle 12:19 PubMed35906707

2022

Oxidative stress-induced premature senescence and aggravated denervated skeletal muscular atrophy by regulating progerin-p53 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxian Xiang,Zongqi You,Xinying Huang,Junxi Dai,Junpeng Zhang,Shuqi Nie,Lei Xu,Junjian Jiang,Jianguang Xu

The journal of physiological sciences : JPS 72:15 PubMed35850611

2022

Tolfenamic acid inhibits ROS-generating oxidase Nox1-regulated p53 activity in intrastriatal injection of malonic acid rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yang,Heling Zhang,Tong Qu,Yi Wang,Yongxian Zhong,Yuchen Yan,Xuefei Ji,Tiayan Chi,Peng Liu,Libo Zou
View all publications
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Product promise

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