Anti-p53 (acetyl K370) antibody [EPR17496] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] - BSA and Azide free (AB240273)

p53 (acetyl K370) was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell extract treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours with ab183544 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab183544 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : HepG2 whole cell extract treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours. Lane 2 : PBS instead of HepG2
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183544).

All lanes:

Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (<a href='/en-us/products/primary-antibodies/p53-acetyl-k370-antibody-epr17496-ab183544'>ab183544</a>)

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K370) antibody [EPR17496] - BSA and Azide free (AB240273)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 NIH/3T3 (Mouse embyro fibroblast cells) cells labeling p53 (acetyl K370) with ab183544 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear and weakly cytoplasm staining on NIH/3T3 cell line.
The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows;
1. ab183544 at 1/500 dilution followed by ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor®488 (IgG H&L) at 1/400 dilution.

[J Cell Biol. May 22, 2006; 173(4) : 533–544.]

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183544).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-p53 (acetyl K370) antibody [EPR17496] - BSA and Azide free (AB240273)

Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab183544 at 1/150 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183544).