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AB62376

Anti-p53 (acetyl K373) antibody [EP356(2)AY]

  • Recombinant
  • RabMAb
  • Lab Essentials
  • 20ul selling size
  • What is this?

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(26 Publications)

Rabbit Recombinant Monoclonal P53 acetyl K373 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 26 publications.

View Alternative Names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)

Immunocytochemistry/Immunofluorescence analysis of HepG2 +/- (etoposide + trichostatin A) (etoposide 30ug/ml 8hr followed by TSA 500ng/ml 4hr) cells labelling p53 (acetyl K373) with purified ab62376 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) primary and ab150120 (AlexaFluor®594 goat anti-mouse) secondary both at 1/1000 dilution. Nuclei were counterstained with DAPI (blue).

For negative control 1, rabbit primary antibody and ab150120 (anti-mouse) secondary antibody were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by ab150077 (anti-rabbit secondary antibody).

Flow Cytometry (Intracellular) - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)

Intracellular Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling p53 (acetyl K373) with purified ab62376 at 1/120 (red). Cells were fixed with 4% paraformaldehyde and then permeabilized with 90% methanol. An Alexa Fluorr®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] (ab172730). Unlabelled sample (blue line) was also used as a control.

Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)
  • WB

Unknown

Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)

Blocking and Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/5000 dilution

Lane 1:

Untreated HepG2 whole cell lysate at 10 µg

Lane 2:

HepG2 treated with 30 ug/mL etoposide for 8 hours,then treated with 500 ng/mL trichostatin A for 4hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)
  • WB

Unknown

Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (AB62376)

Blocking and Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/5000 dilution

Lane 1:

Untreated A431 whole cell lysate at 10 µg

Lane 2:

A431 treated with 30ug/mL etoposide for 8 hours, then treated with 500 ng/mL trichostatin A for 4hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

  • Carrier free

    Anti-p53 (acetyl K373) antibody [EP356(2)AY] - BSA and Azide free

  • 578 PE

    PE Anti-p53 (acetyl K373) antibody [EP356(2)AY]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-p53 (acetyl K373) antibody [EP356(2)AY]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP356(2)AY

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects p53 acetylated on Lysine 373.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/120", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.11% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 35618207, PubMed : 36634798, PubMed : 38653238, PubMed : 9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17189187, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 38653238, PubMed : 9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed : 12524540, PubMed : 17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed : 12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed : 12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).
See full target information TP53 acetyl K373
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:826 PubMed39543094

2024

FXR deficiency induced ferroptosis via modulation of the CBP-dependent p53 acetylation to suppress breast cancer growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Huang,Han Zhao,Hua Dai,Jinying Li,Xiafang Pan,Wentian Pan,Chunhua Xia,Fanglan Liu

Science advances 10:eadp2229 PubMed39454005

2024

Temporal regulation of gene expression through integration of p53 dynamics and modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Lu,Marjan Faizi,Bryon Drown,Alina Simerzin,Joshua François,Gary Bradshaw,Neil Kelleher,Ashwini Jambhekar,Jeremy Gunawardena,Galit Lahav

Nature communications 14:4820 PubMed37563109

2023

Hdac1 and Hdac2 regulate the quiescent state and survival of hair-follicle mesenchymal niche.

Applications

Unspecified application

Species

Unspecified reactive species

Hadas Sibony-Benyamini,Emil Aamar,David Enshell-Seijffers

iScience 26:107003 PubMed37534137

2023

Acetylation halts missense mutant p53 aggregation and rescues tumor suppression in non-small cell lung cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Daxing Xu,Wei Qian,Zhenkun Yang,Zhenhao Zhang,Ping Sun,Quan Wan,Ying Yin,Yaling Hu,Lingli Gong,Bo Zhang,Xusheng Yang,Zhening Pu,Peihua Lu,Jian Zou

Journal of cellular physiology 238:1909-1920 PubMed37357506

2023

PRPF8 controls alternative splicing of PIRH2 to modulate the p53 pathway and survival of human ESCs.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyang Sun,Lingling Zhang,Zhuoqing Fang,Dingyu Liu,Min Shao,Yujie Liu,Bing Liao,Ying Jin

Molecular carcinogenesis 62:754-770 PubMed36920044

2023

Circular RNA ACACA negatively regulated p53-modulated mevalonate pathway to promote colorectal tumorigenesis via regulating miR-193a/b-3p/HDAC3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjiao He,Qiong Liu,Huan Liu,Qian Pei,Hong Zhu

Science advances 8:eabo0412 PubMed35921421

2022

Deacetylation of ATG4B promotes autophagy initiation under starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Liangbo Sun,Haojun Xiong,Lingxi Chen,Xufang Dai,Xiaojing Yan,Yaran Wu,Mingzhen Yang,Meihua Shan,Tao Li,Jie Yao,Wenbin Jiang,Haiyan He,Fengtian He,Jiqin Lian

Frontiers in cell and developmental biology 9:813233 PubMed35174171

2022

Discovery of SIRT7 Inhibitor as New Therapeutic Options Against Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Yaqi Li,Bohao Liu,Chao Ning,Yimin Li,Ying Wang,Zhuan Li

Journal of the American Chemical Society 143:16700-16708 PubMed34592107

2021

Targeted Protein Acetylation in Cells Using Heterobifunctional Molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Wesley W Wang,Li-Yun Chen,Jacob M Wozniak,Appaso M Jadhav,Hayden Anderson,Taylor E Malone,Christopher G Parker

PloS one 15:e0238500 PubMed32976537

2020

Exploring the p53 connection of cervical cancer pathogenesis involving north-east Indian patients.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Aasif Khan,Diptika Tiwari,Anita Dongre, Sadaf,Saad Mustafa,Chandana Ray Das,Sheersh Massey,Purabi Deka Bose,Sujoy Bose,Syed Akhtar Husain
View all publications
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Product promise

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