Anti-p53 (acetyl K382) antibody [EPR358(2)] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-p53 (acetyl K382) antibody [EPR358(2)] - BSA and Azide free (AB219727)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) starved overnight, then treated with 30ug/ml etoposide for 8 hours followed by 500ng/ml TSA for 4 hours cells labeling p53 with purified ab75754 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75754).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K382) antibody [EPR358(2)] - BSA and Azide free (AB219727)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 500ng/ml Trichostatin A for 4 hours cells labeling p53 with purified ab75754 at 1/250 dilution (0.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75754).

• WB

Lab

Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] - BSA and Azide free (AB219727)

p53 K382 acetylation is responsed to cell stress such as DNA damage caused by ultraviolet or ionizing radiation as was described in PMID 9744860 and 11250899.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75754).

All lanes:

Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (<a href='/en-us/products/primary-antibodies/p53-acetyl-k382-antibody-epr3582-ab75754'>ab75754</a>) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) was starved overnight, then treatment with 30ug/ml etoposide for 8 hours followed by 500ng/ml Trichostatin A for 4 hours whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

• WB

Unknown

Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] - BSA and Azide free (AB219727)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75754).

All lanes:

Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (<a href='/en-us/products/primary-antibodies/p53-acetyl-k382-antibody-epr3582-ab75754'>ab75754</a>) at 1/5000 dilution

Lane 1:

HepG2 cell lysates un-treated at 10 µg

Lane 2:

HepG2 cell lysates treated with etopside and TSA at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

• WB

Lab

Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] - BSA and Azide free (AB219727)

p53 K382 acetylation is responsed to cell stress such as DNA damage caused by ultraviolet or ionizing radiation as was described in PMID 9744860 and 11250899.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75754).

All lanes:

Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (<a href='/en-us/products/primary-antibodies/p53-acetyl-k382-antibody-epr3582-ab75754'>ab75754</a>) at 1/1000 dilution

Lane 1:

T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates at 15 µg

Lane 2:

T-47D (Human ductal breast epithelial tumor epithelial cell) was starved overnight, then treatment with 30ug/ml etoposide for 8 hours followed by 500ng/ml Trichostatin A for 4 hours whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false