Rabbit Polyclonal P53 acetyl K386 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TP53 acetyl K386 aa 300 to C-terminus.
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- Nature communications 14:48202023Hdac1 and Hdac2 regulate the quiescent state and survival of hair-follicle mesenchymal niche.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHadas Sibony-Benyamini,Emil Aamar,David Enshell-SeijffersPubMed 37563109
- Communications biology 4:13732021Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Yang,Yun Li,Arup Bhattacharya,Yuesheng ZhangPubMed 34880421
- International journal of molecular sciences 20:2019Stress-Induced, p53-Mediated Tumor Growth Inhibition of Melanoma by Modulated Electrohyperthermia in Mouse Models without Major Immunogenic Effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBalázs Besztercei,Tamás Vancsik,Anett Benedek,Enikő Major,Mbuotidem J Thomas,Csaba A Schvarcz,Tibor Krenács,Zoltán Benyó,Andrea BaloghPubMed 31426515
- Development (Cambridge, England) 145:2018Histone deacetylases 1 and 2 regulate the transcriptional programs of nephron progenitors and renal vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongbing Liu et. al.PubMed 29712641
- Development (Cambridge, England) 145:2018The histone acetyltransferase inhibitor Nir regulates epidermis development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDelphine Duteil et. al.PubMed 29490983
- The Journal of neuroscience : the official journal of the Society for Neuroscience 34:11897-9122014A critical role for interferon regulatory factor 9 in cerebral ischemic stroke.Applications:WBReactive species:MouseHou-Zao Chen,Sen Guo,Zuo-Zhi Li,Yanyun Lu,Ding-Sheng Jiang,Ran Zhang,Hao Lei,Lu Gao,Xiaofei Zhang,Yan Zhang,Lang Wang,Li-Hua Zhu,Mei Xiang,Yan Zhou,Qi Wan,Hailong Dong,De-Pei Liu,Hongliang LiPubMed 25186738
- American journal of physiology. Renal physiology 302:F928-402012Mechanisms of p53 activation and physiological relevance in the developing kidney.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaram Aboudehen,Sylvia Hilliard,Zubaida Saifudeen,Samir S El-DahrPubMed 22237799
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TP53 acetyl K386 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04637
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Alternative names
P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Recommended products
Rabbit Polyclonal P53 acetyl K386 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TP53 acetyl K386 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TP53 acetyl K386 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04637
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab52172 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
- Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
- Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
- Associated diseases and disorders
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (acetyl K386) antibody (ab52172)
ab52172 at 1/50 dilution staining P53 (acetyl K386) in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence (right image) of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K386) antibody (ab52172)
ICC/IF image of ab52172 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52172, 1μg/ml) overnight at +4°C. The secondary antibody (green)was Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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