AB77813

Anti-p53 antibody [9.1]

Lab Essentials
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(13 Publications)

Mouse Monoclonal P53 antibody. Suitable for WB and reacts with Zebrafish samples. Cited in 13 publications.

View Alternative Names

drp53, Cellular tumor antigen p53, Tumor suppressor p53

2 Images

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Western blot - Anti-p53 antibody [9.1] (AB77813)

Western blot analysis using a MOPS gel and 3% milk blocking buffer.

All lanes:

Western blot - Anti-p53 antibody [9.1] (ab77813) at 5 µg/mL

Lane 1:

Untreated Zebrafish Lysate at 10 µg

Lane 2:

Roscovitine-treated Zebrafish Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Exposure time: 20min

Project

Western blot - Anti-p53 antibody [9.1] (AB77813)

All lanes:

Western blot - Anti-p53 antibody [9.1] (ab77813) at 5 µg/mL

Lane 1:

Untreated Zebrafish Lysate at 10 µg

Lane 2:

Roscovitine-treated Zebrafish Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 20 kDa,53 kDa,55 kDa,57 kDa

true

Exposure time: 12min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9.1

Isotype

IgG1

Carrier free

Reacts with

Zebrafish

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression. Positively regulates apoptosis in the early-stage embryo in response to UV irradiation (PubMed : 15529176).

See full target information Cellular tumor antigen p53

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36648336

2023

and mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly J Morgan,Karen Doggett,Fansuo Geng,Stephen Mieruszynski,Lachlan Whitehead,Kelly A Smith,Benjamin M Hogan,Cas Simons,Gregory J Baillie,Ramyar Molania,Anthony T Papenfuss,Thomas E Hall,Elke A Ober,Didier Y R Stainier,Zhiyuan Gong,Joan K Heath

Cell death & disease 12:906 PubMed34611130

2021

The spliceosome factor sart3 regulates hematopoietic stem/progenitor cell development in zebrafish through the p53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Mei Wu,Jing Li,Ping Meng,Jiakui Chen,Zhibin Huang,Jin Xu,Zilong Wen,Wenqing Zhang,Yiyue Zhang

Human molecular genetics 28:4173-4185 PubMed31691804

2019

Splicing factor DHX15 affects tp53 and mdm2 expression via alternate splicing and promoter usage.

Applications

Unspecified application

Species

Unspecified reactive species

John McElderry,Blake Carrington,Kevin Bishop,Erika Kim,Wuhong Pei,Zelin Chen,Ramanagouda Ramanagoudr-Bhojappa,Anupam Prakash,Shawn M Burgess,P Paul Liu,Raman Sood

Ecotoxicology and environmental safety 171:854-862 PubMed30660979

2019

Toxicity and chronic effects of deltamethrin exposure on zebrafish (Danio rerio) as a reference model for freshwater fish community.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan-Adrian Strungaru,Gabriel Plavan,Alin Ciobica,Mircea Nicoara,Madalina Andreea Robea,Carmen Solcan,Adriana Petrovici

PLoS genetics 14:e1007821 PubMed30540754

2018

Multiplexed CRISPR/Cas9-mediated knockout of 19 Fanconi anemia pathway genes in zebrafish revealed their roles in growth, sexual development and fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Ramanagouda Ramanagoudr-Bhojappa,Blake Carrington,Mukundhan Ramaswami,Kevin Bishop,Gabrielle M Robbins,MaryPat Jones,Ursula Harper,Stephen C Frederickson,Danielle C Kimble,Raman Sood,Settara C Chandrasekharappa

Diseases of aquatic organisms 132:57-78 PubMed30530931

2018

Putative histiocytic sarcoma in redfin needlefish Strongylura notata (Beloniformes: Belonidae) in Florida, USA.

Applications

Unspecified application

Species

Unspecified reactive species

Yasunari Kiryu,Jan H Landsberg,Micah D Bakenhaster,Amanda J Tyler-Jedlund,Patrick W Wilson

eLife 7: PubMed30192230

2018

deficiency causes a wide tumor spectrum and increases embryonal rhabdomyosarcoma metastasis in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Myron S Ignatius,Madeline N Hayes,Finola E Moore,Qin Tang,Sara P Garcia,Patrick R Blackburn,Kunal Baxi,Long Wang,Alexander Jin,Ashwin Ramakrishnan,Sophia Reeder,Yidong Chen,Gunnlaugur Petur Nielsen,Eleanor Y Chen,Robert P Hasserjian,Franck Tirode,Stephen C Ekker,David M Langenau

Proceedings of the National Academy of Sciences of 113:E5562-71 PubMed27588899

2016

Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis.

Applications

Species

Zebrafish

Andrew G Cox,Allison Tsomides,Andrew J Kim,Diane Saunders,Katie L Hwang,Kimberley J Evason,Jerry Heidel,Kristin K Brown,Min Yuan,Evan C Lien,Byung Cheon Lee,Sahar Nissim,Bryan Dickinson,Sagar Chhangawala,Christopher J Chang,John M Asara,Yariv Houvras,Vadim N Gladyshev,Wolfram Goessling

eLife 4: PubMed26575287

2015

The human ARF tumor suppressor senses blastema activity and suppresses epimorphic tissue regeneration.

Applications

IHC - Wmt

Species

Zebrafish

Robert G Hesse,Gayle K Kouklis,Nadav Ahituv,Jason H Pomerantz

Nucleic acids research 43:1035-43 PubMed25550427

2015

A naturally occurring 4-bp deletion in the intron 4 of p53 creates a spectrum of novel p53 isoforms with anti-apoptosis function.

Applications

Species

Zebrafish

Hui Shi,Ting Tao,Delai Huang,Zhao Ou,Jun Chen,Jinrong Peng

View all publications

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