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Rabbit Recombinant Monoclonal P53 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 37 publications.


Images

Western blot - Anti-p53 antibody [EPR17343] (AB179477), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR17343] (AB179477), expandable thumbnail
  • Western blot - Anti-p53 antibody [EPR17343] (AB179477), expandable thumbnail
  • Western blot - Anti-p53 antibody [EPR17343] (AB179477), expandable thumbnail
  • Western blot - Anti-p53 antibody [EPR17343] (AB179477), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Alternative names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

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Rabbit Recombinant Monoclonal P53 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 37 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17343
Purification technique
Affinity purification Protein A
Specificity

Based on the results of the knockout validation testing this antibody may not be suitable for IHC-P, Flow Cyt, IP or ChIP. Please contact our Scientific Support team for additional information.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

Associated diseases and disorders

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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6 product images

  • Western blot - Anti-p53 antibody [EPR17343] (ab179477), expandable thumbnail

    Western blot - Anti-p53 antibody [EPR17343] (ab179477)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)

    Lane 2: p53 knockout HAP1 cell lysate (20 μg)

    Lane 3: A431 cell lysate (20 μg)

    Lane 4: Saos-2 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab179477 observed at 53 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.

    ab179477 was shown to specifically react with p53 in wild type HAP1 cells along with additional cross reactive bands. No band was observed with p53 knockout samples were used.

    Wild-type and p53 knockout samples were subjected to SDS-PAGE. ab179477 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta Actin) were diluted to 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-p53 antibody [EPR17343] (ab179477)

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR17343] (ab179477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR17343] (ab179477)

    ab179477 staining p53 in wild-type HAP1 cells (top panel) and p53 knockout HAP1 cells (bottom panel). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab179477 at 1/500 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  • Western blot - Anti-p53 antibody [EPR17343] (ab179477), expandable thumbnail

    Western blot - Anti-p53 antibody [EPR17343] (ab179477)

    All lanes: Western blot - Anti-p53 antibody [EPR17343] (ab179477) at 1/2000 dilution

    Lane 1: Untreated HCT 116 (Human colorectal carcinoma) whole cell lysate

    Lane 2: HCT 116 (Human colorectal carcinoma) whole cell lysate with 0.5μM Doxorubicin for 24hours whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-p53 antibody [EPR17343] (ab179477), expandable thumbnail

    Western blot - Anti-p53 antibody [EPR17343] (ab179477)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-p53 antibody [EPR17343] (ab179477) at 1/20000 dilution

    All lanes: T-47D (Human ductal breast epithelial carcinoma cell line) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

    Observed band size: 44 kDa, 53 kDa

  • Western blot - Anti-p53 antibody [EPR17343] (ab179477), expandable thumbnail

    Western blot - Anti-p53 antibody [EPR17343] (ab179477)

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    Saos-2, PC-3 and HL-60 cells are p53 null cell lines.

    All lanes: Western blot - Anti-p53 antibody [EPR17343] (ab179477) at 1/2000 dilution

    Lane 1: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates at 20 µg

    Lane 2: A431 (Human epidermoid carcinoma) whole cell lysates at 20 µg

    Lane 3: Saos-2 (Human osteosarcoma cell line) whole cell lysates at 20 µg

    Lane 4: HL-60 (Human promyelocytic leukemia cells) whole cell lysates at 20 µg

    Lane 5: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

    Observed band size: 44 kDa

  • Western blot - Anti-p53 antibody [EPR17343] (ab179477), expandable thumbnail

    Western blot - Anti-p53 antibody [EPR17343] (ab179477)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HL-60 (p53 null), NCI-H1299 (p53 null).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21941372, PMID: 21779513, PMID: 25996291).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-p53 antibody [DO-1] - ChIP Grade (Anti-p53 antibody [DO-1] - ChIP Grade ab1101) staining at 1/1000 dilution.

    In Western blot, Anti-p53 antibody [EPR17343] (ab179477) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-TP53 (phospho S376+S377+S392) antibody [RM1161] (Anti-TP53 (phospho S376+S377+S392) antibody [RM1161] ab322465) at 1/1000 dilution

    Lane 1: Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HCT 116 treated with 20uM etoposide for 15 hours whole cell lysate at 20 µg

    Lane 3: Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: A549 treated with 500nM doxorubicin for 24 hours whole cell lysate at 20 µg

    Lane 5: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 7: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 53 kDa, 26-48 kDa, 36 kDa

    Exposure time: 10s

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