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Mouse Monoclonal P53 antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 57 publications.


Images

Western blot - Anti-p53 antibody [PAb 1801] (AB28), expandable thumbnail
  • Western blot - Anti-p53 antibody [PAb 1801] (AB28), expandable thumbnail
  • ELISA - Anti-p53 antibody [PAb 1801] (AB28), expandable thumbnail
  • Western blot - Anti-p53 antibody [PAb 1801] (AB28), expandable thumbnail
  • Western blot - Anti-p53 antibody [PAb 1801] (AB28), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

We recommend using 3% milk as the blocking agent for Western blot.

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Target data

Function

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Alternative names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

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Mouse Monoclonal P53 antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 57 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
PAb 1801
Purification technique
Affinity purification Protein G
Epitope
aa 46-55 of human p53
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This monoclonal p53 antibody has been knockout validated in Western blot. The expected band for p53 was observed in HCT116 cells treated with irinotecan and the band was not seen in TP53 knockout HCT116 cell lysate.

We recommend using Anti-p53 antibody [DO-1] - ChIP Grade ab1101 or Anti-p53 antibody [9D3DE3] ab154036, alternative mouse monoclonal antibodies, to detect human p53 by IHC, ICC/IF or flow cytometry since they perform better in these applications.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

Associated diseases and disorders

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

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5 product images

  • Western blot - Anti-p53 antibody [PAb 1801] (ab28), expandable thumbnail

    Western blot - Anti-p53 antibody [PAb 1801] (ab28)

    Lanes 1-9: Merged (red and green) signal.


    ab28 was shown to specifically react with p53 in wild-type HAP1 and HCT116 cells treated with irinotecan. No band was observed in p53 knockout HAP1 or HCT116 cells. Wild-type and p53 knockout samples, positive and negative controls were subjected to SDS-PAGE. ab28 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted 5 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-p53 antibody [PAb 1801] (ab28) at 5 µg/mL

    Lane 1: Wild-type HCT116 cell lysate at 30 µg

    Lane 2: Wild-type HCT116 + irinotecan (10 μM, 24 hours) cell lysate at 30 µg

    Lane 3: p53 knockout HCT116 cell lysate at 30 µg

    Lane 4: p53 knockout HCT116 + irinotecan (10 μM, 24 hours) cell lysate at 30 µg

    Lane 5: A431 cell lysate (positive control) at 20 µg

    Lane 6: Saos-2 cell lysate (negative control) at 20 µg

    Lane 7: MEF cell lysate at 20 µg

    Lane 8: Wild-type HAP1 cell lysate at 20 µg

    Lane 9: p53 knockout HAP1 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 43 kDa

  • Western blot - Anti-p53 antibody [PAb 1801] (ab28), expandable thumbnail

    Western blot - Anti-p53 antibody [PAb 1801] (ab28)

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab28 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

    We recommend using 3% milk as the blocking agent for Western blot.

    All lanes: Western blot - Anti-p53 antibody [PAb 1801] (ab28) at 5 µg/mL

    All lanes: MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa

    Observed band size: 53 kDa, 60 kDa

    Exposure time: 20min

  • ELISA - Anti-p53 antibody [PAb 1801] (ab28), expandable thumbnail

    ELISA - Anti-p53 antibody [PAb 1801] (ab28)

    Analysis of recombinant p53 protein (Recombinant Human p53 protein ab43615) in MCF7 cells treated with vehicle (VEH)as well as MCF7 cells treated with camptothecin (CAM) and no material (NO) by sandwich ELISA with the use of anti-53 antibody (ab28) as capture and anti-p53 antibody (Anti-p53 antibody [E26] ab32389) as detector.

  • Western blot - Anti-p53 antibody [PAb 1801] (ab28), expandable thumbnail

    Western blot - Anti-p53 antibody [PAb 1801] (ab28)

    All Lanes: Anti-p53 antibody [PAb 1801] (ab28) at 5 μg/ml

    Lane 1 and 10: Wild-type HCT116 cell lysate

    Lane 2 and 11: Wild-type HCT116 + irinotecan (10 μM, 24 hours) cell lysate

    Lane 3 and 12: p53 knockout HCT116 cell lysate

    Lane 4 and 13: p53 knockout HCT116 + irinotecan (10 μM, 24 hours) cell lysate

    Lane 5 and 14: A431 cell lysate (positive control)

    Lane 6 and 15: Saos-2 cell lysate (negative control)

    Lane 7 and 16: MEF cell lysate

    Lane 8 and 17: Wild-type HAP1 cell lysate

    Lane 9 and 18: p53 knockout HAP1 cell lysate

    Lanes 1-9: 1% BSA blocking buffer

    Lanes 10-18: 3% Milk blocking buffer

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) at 1/5000 dilution

    We recommend using 3% milk as the blocking agent for western blot.

    All lanes: Western blot - Anti-p53 antibody [PAb 1801] (ab28)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa

  • Western blot - Anti-p53 antibody [PAb 1801] (ab28), expandable thumbnail

    Western blot - Anti-p53 antibody [PAb 1801] (ab28)

    The image shows a Western blot for ab28, testing the threshold detection of the p53 antibody. The lanes are as follows:

    Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane 5 - 1.5ug of A549 lysate.

    This picture was submitted as part of the review by Craig Carson.

    All lanes: Western blot - Anti-p53 antibody [PAb 1801] (ab28)

    Predicted band size: 43 kDa

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Product protocols

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