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Mouse Monoclonal P53 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 402 publications.
Alternative names=Cellular tumor antigen p53, Tumor suppressor p53, P53, Tp53, Trp53
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted |
| Syrian hamster | Predicted | Predicted | Predicted |
Images
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Monkey, Chinese hamster, Syrian hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes Please note that expression of target protein may be very low without stimulation/treatment (e.g. DNA damaging agent). We recommend using 3% milk as the blocking agent for Western blot. |
Species Mouse | Dilution info 1.00000-5.00000 µg/mL | Notes Please note that expression of target protein may be very low without stimulation/treatment (e.g. DNA damaging agent). We recommend using 3% milk as the blocking agent for Western blot. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Monkey, Chinese hamster, Syrian hamster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Monkey, Chinese hamster, Syrian hamster | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal P53 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 402 publications.
Alternative names=Cellular tumor antigen p53, Tumor suppressor p53, P53, Tp53, Trp53
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
PAb 240
- Purification technique
Affinity purification Protein G
- Epitope
The epitope has been mapped to between amino acids 212 and 217 on human p53 (PMID: 1376364).
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab26 has been knockout validated in Western blot. The expected band was seen in wild type HCT116 cells treated with the DNA damaging agent irinotecan and no band was seen in TP53 knockout HCT116 cells.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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