Anti-p53 antibody [POE316A] - BSA and Azide free

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [POE316A] - BSA and Azide free (AB252786)

This data was developed using ab241566, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse thymus labeling p53 with ab241566 at 1/50 dilution.

The experiment was done in Discovery XT, Ventana-Roche machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.

Giovanna Roncador, Spanish Nacional Cancer Research Centre (CNIO)

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [POE316A] - BSA and Azide free (AB252786)

This data was developed using ab241566, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Wild type mouse fibrosarcoma labeling p53 with ab241566 at 1/50 dilution.

Image A : nuclear staining on Wild type mouse fibrosarcoma

Image B : nearly no staining on p53 knock-out mouse fibrosarcoma

The experiment was done in Discovery XT, Ventana-Roche machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.

Giovanna Roncador, Spanish Nacional Cancer Research Centre (CNIO)

• WB

Supplier Data

Western blot - Anti-p53 antibody [POE316A] - BSA and Azide free (AB252786)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 103 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241566).

All lanes:

Western blot - Anti-p53 antibody [POE316A] (<a href='/en-us/products/primary-antibodies/p53-antibody-poe316a-ab241566'>ab241566</a>) at 1/1000 dilution

Lane 1:

LLC1 (mouse lung carcinoma), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 43 kDa

false

• WB

Lab

Western blot - Anti-p53 antibody [POE316A] - BSA and Azide free (AB252786)

This data was developed using ab241566, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Low or negative sample : normal brain (PMID : 32778161); normal liver (PMID : 10949934); NIH/3T3 (PMID : 10628835).

All lanes:

Western blot - Anti-p53 antibody [POE316A] (<a href='/en-us/products/primary-antibodies/p53-antibody-poe316a-ab241566'>ab241566</a>) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

NIH/3T3 whole cell lysate at 20 µg

Lane 5:

MEF whole cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Exposure time: 180s