AB86383

Anti-p53 BP3 antibody

Name: Anti-p53 BP3 antibody (ab86383) | Abcam
Brand: Abcam Limited
SKU: AB86383

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(4 Publications)

Rabbit Polyclonal p53 BP3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TOPORS aa 950 to C-terminus.

View Alternative Names

LUN, TP53BPL, TOPORS, E3 ubiquitin-protein ligase Topors, RING-type E3 ubiquitin transferase Topors, SUMO1-protein E3 ligase Topors, Topoisomerase I-binding RING finger protein, Topoisomerase I-binding arginine/serine-rich protein, Tumor suppressor p53-binding protein 3, p53-binding protein 3, p53BP3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 BP3 antibody (AB86383)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 BP3 antibody (AB86383)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling p53 BPS with ab86383 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-p53 BP3 antibody (AB86383)

Detection of Human p53 BP3 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86383 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-p53 BP3 antibody (ab86383)

Predicted band size: 119 kDa

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Western blot - Anti-p53 BP3 antibody (AB86383)

All lanes:

Western blot - Anti-p53 BP3 antibody (ab86383) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 119 kDa

Observed band size: 171 kDa

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Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human TOPORS aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NS56

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-15 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P53 BP3 also known as BCCIP (BRCA2 and CDKN1A interacting protein) weighs approximately 36 kilodaltons. Researchers find it mainly expressed in actively dividing cells within tissues like testis ovary and several cancer cell lines indicating its potential significance in cell cycle regulation and maintenance. p53 BP3 interacts with other components of the cellular machinery to fulfill its roles and it has garnered interest due to its association with p53 a well-known tumor suppressor protein.

Biological function summary

Alongside being associated with cell cycle checkpoints p53 BP3 participates in DNA damage response and repair processes. It works in close association with proteins like BRCA2 and p21 potentially forming part of a complex that ensures genomic stability and assists in cell cycle arrest in response to DNA damage. Through these interactions p53 BP3 contributes to the effective management and repair of DNA lesions supporting the orderly progression through the cell cycle.

Pathways

Cell cycle and DNA damage response pathways both involve p53 BP3. In the p53 signaling pathway it interacts with p53 which coordinates cell cycle arrest and DNA repair. In the DNA damage response pathway BRCA2 and p21 are closely linked to p53 BP3 facilitating homologous recombination and checkpoint control. p53 BP3’s role here ensures proper cellular responses to prevent mutations from being passed on during cell division.

P53 BP3 has been implicated in various cancers as its dysfunction can lead to aberrant cell cycle control and insufficient DNA repair. This link emphasizes its relationship with proteins like BRCA2 often seen with breast cancer predisposition. Additionally decreased levels or mutations of p53 BP3 have connections to neurodegenerative disorders where cellular stress and compromised DNA repair mechanisms contribute to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.

See full target information TOPORS

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

RSC chemical biology 6:1759-1771 PubMed40978459

2025

Cell-based high-throughput screening using a target-NanoLuc fusion construct to identify molecular glue degraders of c-Myc oncoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Muyu Xu,Jinying Qiu,Lin Tan,Jiayu Xu,Yi Wang,Wenyue Kong,Hongda Liao,Anran Chen,Xiaolan Chen,Jiying Zhang,Cookson K C Chiu,Meiying Zhang,Yingying Tian,Caohui Li,Biao Ma,Leiming Wang,Jingpeng Fu,Seung H Choi,Jeffrey Hill,Weijun Shen

The Journal of cell biology 224: PubMed40239066

2025

PML mutants from arsenic-resistant patients reveal SUMO1-TOPORS and SUMO2/3-RNF4 degradation pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ellis G Jaffray,Michael H Tatham,Barbara Mojsa,Anna Plechanovová,Alejandro Rojas-Fernandez,Julio C Y Liu,Niels Mailand,Adel F M Ibrahim,Graeme Ball,Iain M Porter,Ronald T Hay

Frontiers in oncology 14:1485802 PubMed39697236

2024

miR-198 targets : implications for oral squamous cell carcinoma pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Pankhuri Kaushik,Radha Mishra,Champaka Gopal,Arun Kumar

Cancer biomarkers : section A of Disease markers 38:287-300 PubMed37955079

2023

Secreted frizzled related-protein 2 is prognostic for human pancreatic cancer patient survival and is associated with fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Julie B Siegel,Patrick Nasarre,Lillian Hsu,Rupak Mukherjee,Meghan Gormley,Bailey Richardson,Imran Khan,Jordan E Morningstar,Eleanor Hilliard,John P O'Bryan,Kristi L Helke,Laura Spruill,Nathan G Dolloff,Nancy Klauber-DeMore

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p53 BP3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 BP3 antibody (AB86383)

Immunoprecipitation - Anti-p53 BP3 antibody (AB86383)

Western blot - Anti-p53 BP3 antibody (AB86383)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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