Rabbit Recombinant Monoclonal P53 phospho S20 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples.
Images
![Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--immunoprecipitation-img41342.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325)")
![Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--western-blot-img21009.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325)")
![Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--western-blot-img70269.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325)")
![Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--dot-blot-img18183.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (AB249325)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Alternative names
P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Recommended products
Rabbit Recombinant Monoclonal P53 phospho S20 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2156(2)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249325 is the carrier-free version of Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
- Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
- Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
- Associated diseases and disorders
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--immunoprecipitation-img41342.jpg "Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)")
Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
This data was developed using Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454&
44; the same antibody clone in a different buffer formulation.
Detection of p53 Western Blot of Immunprecipitate. T47D cells treated with etoposide lysate immunoprecipitated using Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454 at 1/10 dilution followed by HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] (Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454) at 1/1000 dilution
All lanes: Immunoprecipitation pellet from T47-D cells treated with etoposide lysate at 10 µg
Predicted band size: 43 kDa
![Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--western-blot-img21009.jpg "Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)")
Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
This data was developed using Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454) at 1/1000 dilution
Lane 1: T-47D (human mammary gland ductal carcinoma) treated with Etoposide whole cell lysate at 10 µg
Lane 2: Untreated T47D (human mammary gland ductal carcinoma) whole cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa
Exposure time: 1min
![Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--western-blot-img70269.jpg "Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)")
Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
This data was developed using Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454, the same antibody clone in a different buffer formulation.
Lower panel shows labeling with an anti-p53 C terminal antibody.
All lanes: Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454) at 1/1000 dilution
Lane 1: T-47D cell lysate at 10 µg
Lane 2: T-47D cell lysate treated with etoposide. at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 43 kDa
![Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325), expandable thumbnail](https://content.abcam.com/products/images/p53-phospho-s20-antibody-epr21562-bsa-and-azide-free-ab249325--dot-blot-img18183.jpg "Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)")
Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
This data was developed using Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: p53 (pS20) phospho peptide Lane 2 sample: p53 non-phospho peptide Exposure time: 10 seconds
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