Name: Anti-p53 (phospho S392) antibody [EP155Y]
SKU: ab33889
Rating: 3 (1 reviews)

Rabbit Recombinant Monoclonal P53 phospho S392 antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 22 publications.

Images

Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (AB33889), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Dot	WB	ICC/IF	IHC-P
Human	Tested	Expected	Tested	Not recommended	Tested
Mouse	Expected	Expected	Tested	Not recommended	Expected
Rat	Expected	Expected	Tested	Not recommended	Expected
Synthetic peptide	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes For unpurified use at 1/80. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes -
Species Rat	Dilution info 1/1000 - 1/5000	Notes -
Species Human	Dilution info 1/1000 - 1/5000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

See full target information TP53 phospho S392

Function

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Alternative names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Recommended products

Rabbit Recombinant Monoclonal P53 phospho S392 antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 22 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP155Y
Purification technique: Affinity purification Protein A
Specificity: This antibody is specific for p53 phosphorylated on Serine 392.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

Associated diseases and disorders

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889) at 1/1000 dilution
Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg
Lane 2: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates, then the membrane was incubated with alkaline phosphatase at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Immunoprecipitation - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
ab33889 (purified) at 1:20 dilution (0.6μg) immunoprecipitating p53 in 293T whole cell lysate.

Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate, 10μg
Lane 2 (+): ab33889 & 293T whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab33889 in 293T whole cell lysate.

For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling p53 with Purified ab33889 at 1:250 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889) at 1/1000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates, then the membrane was incubated with alkaline phosphatase at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889) at 1/1000 dilution
Lane 1: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg
Lane 2: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates, then the membrane was incubated with alkaline phosphatase. at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa
Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889) at 1/2000 dilution
Lane 1: A431 whole cell lysate at 10 µg
Lane 2: A431 treated with 1μg/ml doxorubicin for 24 hours whole cell lysate at 10 µg
Lane 3: A431 treated with 1μg/ml doxorubicin for 24 hours whole cell lysate, the membrane was incubated with alkaline phosphatase at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa
Exposure time: 15s
Dot Blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Dot blot analysis of p53 (pS392) peptide (Lane 1) and p53 non-phospho peptide (Lane 2) labelling p53 (pS392) with unpurified ab33889 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889) at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate - untreated at 10 µg
Lane 2: HEK-293 whole cell lysate - treated with Alkaline Phosphatase at 10 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Unpurified ab33889, at a 1/100 dilution, staining p53 in paraffin embedded human prostate adenocarcinoma tissue by Immunohistochemistry.
Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Lanes 1 and 5: Extract of Hek293T incubated with etoposide (7.5 μg).
Lanes 2 and 6: Lambda phosphatase (400 times-diluted)-treated extract of Hek293T incubated with etoposide (7.5 μg).
Lanes 3 and 7: Lambda phosphatase (100 times-diluted)-treated extract of Hek293T incubated with etoposide (7.5 μg).
Lanes 4 and 8: Lambda phosphatase (25 times-diluted)-treated extract of Hek293T incubated with etoposide (7.5 μg).
SDS PAGE performed under reducing conditions (100mM DTT, sample heated at 50°C).
Primary:
Lanes 1-4: Anti p53 (phosphoS392) antibody (ab33889, unpurified) at 1/2000 dilution.
Lanes 5-8: Anti p53 antibody (Anti-p53 antibody [DO-1] - ChIP Grade ab1101) at 1/2500 dilution.
Secondary:
Lanes 1-4: HRP-conjugated goat anti-rabbit IgG (H&L) at 1/10000.
Lanes 5-8: HRP-conjugated goat anti-mouse IgG (H&L) at 1/10000.
Blocked in 5% milk in PBS for 3 hours at room temperature.
Incubated with the primary antibody in 5% BSA + 50mM Tris pH 7.5 + 0.05% Tween-20 overnight at 4°C.
Incubated with the secondary antibody in blocking buffer for 2 hours at room temperature.
All lanes: Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 53 kDa
Exposure time: 1min
Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889)
All lanes: Western blot - Anti-p53 (phospho S392) antibody [EP155Y] (ab33889) at 1/500 dilution
Lane 1: MCF7 cell lysate untreated.
Lane 2: MCF7 cell lysate treated with 5 ug/ml Actinomycin for 3hrs.
Lane 3: MCF7 cell lysate treated with 5 ug/ml Actinomycin for 6hrs.
Lane 4: MCF7 cell lysate treated with 5 ug/ml Actinomycin for 18hrs.
Predicted band size: 43 kDa
Observed band size: 53 kDa