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AB183546

Anti-p53 (phospho T55) antibody [EPR17729]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

5

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal P53 phospho T55 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 2 publications.

View Alternative Names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

4 Images
Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)
  • WB

AbReview46693****

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)

Western blot analysis of HCT116 p53wt and HCT116 p53-/- cells were treated with DMSO (Control) or 15/24h with 500 nM doxorubicin (ab120629) labelling p53 (phospho T55) with ab183546 at 1/2500. A HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/5000). GAPDH (ab128915) was used as protein loading control.

All lanes:

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)

Predicted band size: 43 kDa

Observed band size: 35 kDa,55 kDa

true

Exposure time: 30s

This image is courtesy of an Abreview submitted by Christian Marx

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)
  • WB

Supplier Data

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)

All lanes:

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546) at 1/1000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes at 10 µg

Lane 2:

Untreated HEK293 (human embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Exposure time: 15s

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)
  • WB

Supplier Data

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546) at 1/1000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 15 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate membrane incubated with phosphatase at 15 µg

Lane 3:

Whole cell lysate from HEK-293 (Human epithelial cell line from embryonic kidney) cells, treated with UV at 60J/m2 for 2 hours at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Exposure time: 10s

Dot Blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)
  • Dot

Supplier Data

Dot Blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)

Dot blot analysis of p53 (phospho T55) using ab183546 at 1/1000 dilution followed by Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated secondary antibody at 1/1000 dilution.

Lane 1 : Phospho peptide

Lane 2 : Non-phospho peptide

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time of 3 minutes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17729

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 35618207, PubMed : 36634798, PubMed : 38653238, PubMed : 9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17189187, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 38653238, PubMed : 9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed : 12524540, PubMed : 17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed : 12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed : 12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).
See full target information TP53 phospho T55
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:826 PubMed39543094

2024

FXR deficiency induced ferroptosis via modulation of the CBP-dependent p53 acetylation to suppress breast cancer growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Huang,Han Zhao,Hua Dai,Jinying Li,Xiafang Pan,Wentian Pan,Chunhua Xia,Fanglan Liu

eLife 11: PubMed35924897

2022

Coupling to short linear motifs creates versatile PME-1 activities in PP2A holoenzyme demethylation and inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yitong Li,Vijaya Kumar Balakrishnan,Michael Rowse,Cheng-Guo Wu,Anastasia Phoebe Bravos,Vikash K Yadav,Ylva Ivarsson,Stefan Strack,Irina V Novikova,Yongna Xing
View all publications
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Product promise

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