AB8105

Anti-p53R2 antibody

Name: Anti-p53R2 antibody (ab8105) | Abcam
Brand: Abcam Limited
SKU: AB8105
Price: 605 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(30 Publications)

Rabbit Polyclonal p53R2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human RRM2B aa 1-50.

View Alternative Names

P53R2, RRM2B, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2

1 Images

Supplier Data

Western blot - Anti-p53R2 antibody (AB8105)

All lanes:

Western blot - Anti-p53R2 antibody (ab8105) at 1 µg/mL

Lane 1:

3T3 cell lysate with absence of blocking peptide

Lane 2:

3T3 cell lysate with presence of blocking peptide

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human RRM2B aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q7LG56

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P53R2 also known as RRM2B is a ribonucleotide reductase subunit with a molecular mass of 41 kDa. This protein is primarily expressed in the cytoplasm and mitochondria of various cell types including liver and kidney tissues. Mechanically p53R2 functions by reducing ribonucleotides into deoxyribonucleotides which are necessary for DNA synthesis and repair. This process is important for maintaining genomic stability especially under conditions of cellular stress or damage.

Biological function summary

P53R2 plays a role in the synthesis of deoxyribonucleotide triphosphates (dNTPs) for DNA repair. It forms part of a ribonucleotide reductase complex working with other subunits to ensure cells have sufficient dNTP pools for DNA replication and repair. The presence of p53R2 is integral for DNA damage response pathways where it gets activated to supply dNTPs during periods when the p53 tumor suppressor pathway senses DNA damage and activates DNA repair processes.

Pathways

P53R2 is involved in DNA damage response and cell cycle regulation. It interacts with the tumor suppressor protein p53 especially during instances of genotoxic stress facilitating DNA repair and cell cycle arrest to prevent propagation of damaged DNA. Additionally p53R2 relates to proteins such as p21 which are components of the p53 pathway influencing cell cycle arrest and allowing time for DNA repair before cell division resumes.

Researchers link p53R2 to cancer and mitochondrial depletion syndrome. In cancer mutations or dysregulated expression of p53R2 can impair DNA repair leading to genomic instability and tumorigenesis. The protein associates with p53 in these processes and its dysfunction may contribute to cancer progression. Furthermore mutations in p53R2 have implications in mitochondrial depletion syndrome where reduced functionality can affect mitochondrial DNA maintenance and cellular energy metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.

See full target information RRM2B

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:12351-12377 PubMed39315696

2024

p53-dependent crosstalk between DNA replication integrity and redox metabolism mediated through a NRF2-PARP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gamal Ahmed Elfar,Obed Aning,Tsz Wai Ngai,Pearlyn Yeo,Joel Wai Kit Chan,Shang Hong Sim,Leonard Goh,Ju Yuan,Cheryl Zi Jin Phua,Joanna Zhen Zhen Yeo,Shi Ya Mak,Brian Kim Poh Goh,Pierce Kah-Hoe Chow,Wai Leong Tam,Ying Swan Ho,Chit Fang Cheok

Cancers 15: PubMed37444452

2023

Radiation-Induced Cellular Senescence Reduces Susceptibility of Glioblastoma Cells to Oncolytic Vaccinia Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Quinn T Storozynsky,Xuefei Han,Shae Komant,Kate C Agopsowicz,Kyle G Potts,Armin M Gamper,Roseline Godbout,David H Evans,Mary M Hitt

EMBO molecular medicine 12:e10419 PubMed31950591

2020

Ribonucleotide reductase inhibitors suppress SAMHD1 ara-CTPase activity enhancing cytarabine efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Sean G Rudd,Nikolaos Tsesmetzis,Kumar Sanjiv,Cynthia Bj Paulin,Lakshmi Sandhow,Juliane Kutzner,Ida Hed Myrberg,Sarah S Bunten,Hanna Axelsson,Si Min Zhang,Azita Rasti,Petri Mäkelä,Si'Ana A Coggins,Sijia Tao,Sharda Suman,Rui M Branca,Georgios Mermelekas,Elisée Wiita,Sun Lee,Julian Walfridsson,Raymond F Schinazi,Baek Kim,Janne Lehtiö,Georgios Z Rassidakis,Katja Pokrovskaja Tamm,Ulrika Warpman-Berglund,Mats Heyman,Dan Grandér,Sören Lehmann,Thomas Lundbäck,Hong Qian,Jan-Inge Henter,Torsten Schaller,Thomas Helleday,Nikolas Herold

OncoTargets and therapy 12:8367-8378 PubMed31632084

2019

miR-942 promotes proliferation and metastasis of hepatocellular carcinoma cells by inhibiting RRM2B.

Applications

Unspecified application

Species

Unspecified reactive species

Qifan Zhang,Bili Zhu,Jianping Qian,Kai Wang,Jie Zhou

Frontiers in oncology 9:956 PubMed31637211

2019

Integrative Analysis Reveals Across-Cancer Expression Patterns and Clinical Relevance of Ribonucleotide Reductase in Human Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfeng Ding,Tingting Zhong,Min Wang,Xueping Xiang,Guoping Ren,Zhongjuan Jia,Qinghui Lin,Qian Liu,Jingwen Dong,Linrong Li,Xiawei Li,Haiping Jiang,Lijun Zhu,Haoran Li,Dejun Shen,Lisong Teng,Chen Li,Jimin Shao

Cancer management and research 10:3505-3511 PubMed30271199

2018

CD8+ tumor-infiltrating lymphocytes as a novel prognostic biomarker in lung sarcomatoid carcinoma, a rare subtype of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewei Chen,Qingmei He,Jun Liu,Yongbo Xiao,Canhua Xiao,Keming Chen,Dan Xie,Xinke Zhang

Nature chemical biology 14:943-954 PubMed30150681

2018

Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Fu,Marcus J C Long,Somsinee Wisitpitthaya,Huma Inayat,Timothy M Pierpont,Islam M Elsaid,Jordana C Bloom,Joaquin Ortega,Robert S Weiss,Yimon Aye

BMC cancer 17:855 PubMed29246119

2017

Overexpression of p53R2 is associated with poor prognosis in lung sarcomatoid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewei Chen,Yongbo Xiao,Xiaoyan Cai,Jun Liu,Keming Chen,Xinke Zhang

Oncotarget 8:101572-101590 PubMed29254187

2017

Transcriptomic pathway analysis of urokinase receptor silenced breast cancer cells: a microarray study.

Applications

Unspecified application

Species

Unspecified reactive species

Pavan B Narayanaswamy,Tapan K Baral,Hermann Haller,Inna Dumler,Kshitish Acharya,Yulia Kiyan

Cell cycle (Georgetown, Tex.) 16:1673-1682 PubMed28841361

2017

p53R2 overexpression in cervical cancer promotes AKT signaling and EMT, and is correlated with tumor progression, metastasis and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Jiang,Rui Xu,Xiao-Xing Li,Yan-Yan Wang,Wen-Qian Liang,Ju-Deng Zeng,Shan-Shan Zhang,Xiao-Yi Xu,Yang Yang,Mei-Yin Zhang,Hui-Yun Wang,X F Steven Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p53R2 antibody

Western blot - Anti-p53R2 antibody (AB8105)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

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