Rabbit Recombinant Monoclonal p53R2 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--western-blot-img142030.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29397.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090)")
![Immunoprecipitation - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--immunoprecipitation-img3802.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090)")
![Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--western-blot-img5888.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (AB249090)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
Alternative names
Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2, RRM2B, P53R2
Recommended products
Rabbit Recombinant Monoclonal p53R2 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Alternative names
Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2, RRM2B, P53R2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR8816
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249090 is the carrier-free version of Anti-p53R2 antibody [EPR8816] ab154194.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
P53R2 also known as RRM2B is a ribonucleotide reductase subunit with a molecular mass of 41 kDa. This protein is primarily expressed in the cytoplasm and mitochondria of various cell types including liver and kidney tissues. Mechanically p53R2 functions by reducing ribonucleotides into deoxyribonucleotides which are necessary for DNA synthesis and repair. This process is important for maintaining genomic stability especially under conditions of cellular stress or damage.
- Biological function summary
P53R2 plays a role in the synthesis of deoxyribonucleotide triphosphates (dNTPs) for DNA repair. It forms part of a ribonucleotide reductase complex working with other subunits to ensure cells have sufficient dNTP pools for DNA replication and repair. The presence of p53R2 is integral for DNA damage response pathways where it gets activated to supply dNTPs during periods when the p53 tumor suppressor pathway senses DNA damage and activates DNA repair processes.
- Pathways
P53R2 is involved in DNA damage response and cell cycle regulation. It interacts with the tumor suppressor protein p53 especially during instances of genotoxic stress facilitating DNA repair and cell cycle arrest to prevent propagation of damaged DNA. Additionally p53R2 relates to proteins such as p21 which are components of the p53 pathway influencing cell cycle arrest and allowing time for DNA repair before cell division resumes.
- Associated diseases and disorders
Researchers link p53R2 to cancer and mitochondrial depletion syndrome. In cancer mutations or dysregulated expression of p53R2 can impair DNA repair leading to genomic instability and tumorigenesis. The protein associates with p53 in these processes and its dysfunction may contribute to cancer progression. Furthermore mutations in p53R2 have implications in mitochondrial depletion syndrome where reduced functionality can affect mitochondrial DNA maintenance and cellular energy metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--western-blot-img142030.jpg "Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)")
Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)
This data was developed using the same antibody clone in a different buffer formulation (Anti-p53R2 antibody [EPR8816] ab154194).
Lanes 1- 2: Merged signal (red and green). Green - Anti-p53R2 antibody [EPR8816] ab154194 observed at 40 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.
Anti-p53R2 antibody [EPR8816] ab154194 was shown to react with p53R2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human RRM2B (p53R2) knockout HeLa cell line ab261769 (knockout cell lysate Human RRM2B (p53R2) knockout HeLa cell lysate ab257215) was used. Wild-type HeLa and RRM2B knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-p53R2 antibody [EPR8816] ab154194 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-p53R2 antibody [EPR8816] (Anti-p53R2 antibody [EPR8816] ab154194) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 40 µg
Lane 2: RRM2B knockout HeLa cell lysate at 40 µg
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29397.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling p53R2 with purified Anti-p53R2 antibody [EPR8816] ab154194 at 1/500 dilution (0.22 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p53R2 antibody [EPR8816] ab154194).
![Immunoprecipitation - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--immunoprecipitation-img3802.jpg "Immunoprecipitation - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)")
Immunoprecipitation - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)
Anti-p53R2 antibody [EPR8816] ab154194 (purified ) at 1/20 dilution (0.5ug) immunoprecipitating p53R2 in MCF7 whole cell lysate.
Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): Anti-p53R2 antibody [EPR8816] ab154194 & MCF7 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p53R2 antibody [EPR8816] ab154194 in MCF7 whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p53R2 antibody [EPR8816] ab154194).
All lanes: Immunoprecipitation - Anti-p53R2 antibody [EPR8816] (Anti-p53R2 antibody [EPR8816] ab154194)
Predicted band size: 40 kDa
![Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090), expandable thumbnail](https://content.abcam.com/products/images/p53r2-antibody-epr8816-bsa-and-azide-free-ab249090--western-blot-img5888.jpg "Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)")
Western blot - Anti-p53R2 antibody [EPR8816] - BSA and Azide free (ab249090)
This data was developed using the same antibody clone in a different buffer formulation (Anti-p53R2 antibody [EPR8816] ab154194).
Lanes 1- 2: Merged signal (red and green). Green - Anti-p53R2 antibody [EPR8816] ab154194 observed at 40 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.
Anti-p53R2 antibody [EPR8816] ab154194 was shown to react with p53R2 in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line Human RRM2B (p53R2) knockout HCT116 cell line ab266897 (knockout cell lysate Human RRM2B (p53R2) knockout HCT116 cell lysate ab257216) was used. Wild-type HCT116 and RRM2B knockout HCT116 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-p53R2 antibody [EPR8816] ab154194 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-p53R2 antibody [EPR8816] (Anti-p53R2 antibody [EPR8816] ab154194) at 1/1000 dilution
Lane 1: Wild-type HCT116 cell lysate at 20 µg
Lane 2: RRM2B knockout HCT116 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
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