AB308366

Anti-p53R2 antibody [HL1400]

Recombinant
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Rabbit Recombinant Monoclonal p53R2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human RRM2B.

View Alternative Names

P53R2, RRM2B, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2

4 Images

Immunohistochemistry paraffin embedded sections - Anti-p53R2 antibody [HL1400] (AB308366)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-p53R2 antibody [HL1400] (AB308366)

Immunohistochemical analysis of mouse spleen tissue labeling p53R2 using ab308366 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-p53R2 antibody [HL1400] (AB308366)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-p53R2 antibody [HL1400] (ab308366) at 1/1000 dilution

All lanes:

Daudi cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40.7 kDa

false

Supplier Data

Western blot - Anti-p53R2 antibody [HL1400] (AB308366)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (ab308366) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-p53R2 antibody [HL1400] (ab308366) at 1/1000 dilution

Lane 1:

Neuro2A cell lysate at 30 µg

Lane 2:

NIH-3T3 cell lysate at 30 µg

Lane 3:

Raw264.7 cell lysate at 30 µg

Lane 4:

C2C12 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40.7 kDa

false

Supplier Data

Western blot - Anti-p53R2 antibody [HL1400] (AB308366)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-p53R2 antibody [HL1400] (ab308366) at 1/1000 dilution

All lanes:

Rat2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40.7 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1400

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RRM2B. The exact immunogen used to generate this antibody is proprietary information.

Q7LG56

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P53R2 also known as RRM2B is a ribonucleotide reductase subunit with a molecular mass of 41 kDa. This protein is primarily expressed in the cytoplasm and mitochondria of various cell types including liver and kidney tissues. Mechanically p53R2 functions by reducing ribonucleotides into deoxyribonucleotides which are necessary for DNA synthesis and repair. This process is important for maintaining genomic stability especially under conditions of cellular stress or damage.

Biological function summary

P53R2 plays a role in the synthesis of deoxyribonucleotide triphosphates (dNTPs) for DNA repair. It forms part of a ribonucleotide reductase complex working with other subunits to ensure cells have sufficient dNTP pools for DNA replication and repair. The presence of p53R2 is integral for DNA damage response pathways where it gets activated to supply dNTPs during periods when the p53 tumor suppressor pathway senses DNA damage and activates DNA repair processes.

Pathways

P53R2 is involved in DNA damage response and cell cycle regulation. It interacts with the tumor suppressor protein p53 especially during instances of genotoxic stress facilitating DNA repair and cell cycle arrest to prevent propagation of damaged DNA. Additionally p53R2 relates to proteins such as p21 which are components of the p53 pathway influencing cell cycle arrest and allowing time for DNA repair before cell division resumes.

Researchers link p53R2 to cancer and mitochondrial depletion syndrome. In cancer mutations or dysregulated expression of p53R2 can impair DNA repair leading to genomic instability and tumorigenesis. The protein associates with p53 in these processes and its dysfunction may contribute to cancer progression. Furthermore mutations in p53R2 have implications in mitochondrial depletion syndrome where reduced functionality can affect mitochondrial DNA maintenance and cellular energy metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.

See full target information RRM2B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p53R2 antibody [HL1400]

Immunohistochemistry paraffin embedded sections - Anti-p53R2 antibody [HL1400] (AB308366)

Western blot - Anti-p53R2 antibody [HL1400] (AB308366)

Western blot - Anti-p53R2 antibody [HL1400] (AB308366)

Western blot - Anti-p53R2 antibody [HL1400] (AB308366)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise