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AB1766

Anti-p55/DCAF1 antibody

4

(2 Reviews)

|

(8 Publications)

Rabbit Polyclonal CAF1 antibody. Cited in 8 publications.

View Alternative Names

Caf1, CG4236, Caf1-55, Chromatin assembly factor 1 p55 subunit, CAF-1 p55 subunit, CAF-1, Nucleosome-remodeling factor 55 kDa subunit, NURF-55

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p55/DCAF1 protein also known as VprBP or HVprBP exhibits critical mechanical roles within the cell. With a mass of approximately 150 kDa this protein is a component of the DDB1–CUL4 E3 ubiquitin ligase complex. It is expressed in various tissues with significant occurrence in the brain heart and skeletal muscle. p55/DCAF1 acts as an adaptor that guides substrate proteins to the CUL4-DDB1 complex for ubiquitination influencing protein degradation and homeostasis regulation in the cell.
Biological function summary

Functions of p55/DCAF1 extend beyond mere ubiquitination facilitation. It plays a role in transcriptional regulation by interacting with nuclear receptors and histone-modifying enzymes. This protein also contributes to chromatin remodeling hinting its involvement in gene expression control. Involvement in DNA damage response adds to its biological significance connecting it with cellular repair processes. As part of a multiprotein complex p55/DCAF1 cooperates with proteins such as DDB1 in maintaining genomic integrity.

Pathways

The influence of p55/DCAF1 finds its importance in the cell cycle and DNA repair pathways. In the context of DNA damage response p55/DCAF1 works intricately with other proteins like ATR and CHK1 to monitor and respond to DNA replication stress. Additionally its participative function in the ubiquitin-proteasome pathway links it firmly to broader protein homeostasis networks where it modulates signals involving key regulators such as p53 and beta-catenin.

Researchers have found connections involving p55/DCAF1 in certain cancers and viral infections. Associations with oncogenic pathways suggest that dysregulation of this protein may contribute to tumorigenesis possibly due to its influence on cell cycle control and genomic stability. Furthermore its interaction with viral proteins such as the HIV-1 Vpr has implicated p55/DCAF1 in viral replication and pathogenesis revealing its potential as a therapeutic target in chronic viral diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the nucleosome remodeling and deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the nucleosome remodeling factor (NURF) complex, which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin; and the polycomb group (PcG) repressor complex ESC-E(Z), which promotes repression of homeotic genes during development. Also required for transcriptional repression of E2F target genes by E2f2 and Rbf or Rbf2.
See full target information Caf1-55
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 237:815-823 PubMed34378805

2021

Merlin functions as a critical regulator in Staphylococcus aureus-induced osteomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zubin Zhou,Yuanliang Chen,Hong Sung Min,Yongbai Wan,Haojie Shan,Yiwei Lin,Wenyang Xia,Fuli Yin,Chaolai Jiang,Xiaowei Yu

Journal of inflammation research 14:1667-1676 PubMed33953594

2021

Ddb1-Cullin4-Associated-Factor 1 in Macrophages Restricts the -Induced Osteomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zong,Haojie Shan,Fuli Yin,Xin Ma,Chaolai Jiang,Nan Wang,Lihui Zhou,Yiwei Lin,Zubin Zhou,Xiaowei Yu

Developmental cell 56:1000-1013.e6 PubMed33725482

2021

Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ramona Lattao,Hélène Rangone,Salud Llamazares,David M Glover

Cells 9: PubMed32111103

2020

The RNA Helicase Belle (DDX3) Non-Autonomously Suppresses Germline Tumorigenesis Via Regulation of a Specific mRNA Set.

Applications

Unspecified application

Species

Unspecified reactive species

Alexei A Kotov,Baira K Godneeva,Oxana M Olenkina,Vladimir E Adashev,Mikhail V Trostnikov,Ludmila V Olenina

Molecular biology and evolution 30:1853-66 PubMed23645555

2013

Subfunctionalization via adaptive evolution influenced by genomic context: the case of histone chaperones ASF1a and ASF1b.

Applications

Unspecified application

Species

Unspecified reactive species

Federico Abascal,Armelle Corpet,Zachary A Gurard-Levin,David Juan,Françoise Ochsenbein,Daniel Rico,Alfonso Valencia,Geneviève Almouzni

Molecular and cellular biology 32:2110-20 PubMed22451490

2012

A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes.

Applications

WB, IP

Species

Drosophila melanogaster, Drosophila melanogaster

Hangnoh Lee,Linda Ragusano,Ana Martinez,Jagdip Gill,Dessislava K Dimova

Molecular and cellular biology 30:2563-77 PubMed20176807

2010

Drosophila RB proteins repress differentiation-specific genes via two different mechanisms.

Applications

WB

Species

Drosophila melanogaster

Hangnoh Lee,Katsuhito Ohno,Yekaterina Voskoboynik,Linda Ragusano,Anna Martinez,Dessislava K Dimova

Molecular cell 17:301-11 PubMed15664198

2005

Human Asf1 regulates the flow of S phase histones during replicational stress.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Groth,Dominique Ray-Gallet,Jean-Pierre Quivy,Jiri Lukas,Jiri Bartek,Geneviève Almouzni
View all publications
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