Rabbit Polyclonal CAF1 antibody. Cited in 8 publications.
Preservative: 0.02% Sodium azide
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the nucleosome remodeling and deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the nucleosome remodeling factor (NURF) complex, which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin; and the polycomb group (PcG) repressor complex ESC-E(Z), which promotes repression of homeotic genes during development. Also required for transcriptional repression of E2F target genes by E2f2 and Rbf or Rbf2.
Caf1, CG4236, Caf1-55, Chromatin assembly factor 1 p55 subunit, CAF-1 p55 subunit, CAF-1, Nucleosome-remodeling factor 55 kDa subunit, NURF-55
Rabbit Polyclonal CAF1 antibody. Cited in 8 publications.
Preservative: 0.02% Sodium azide
Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.
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The p55/DCAF1 protein also known as VprBP or HVprBP exhibits critical mechanical roles within the cell. With a mass of approximately 150 kDa this protein is a component of the DDB1–CUL4 E3 ubiquitin ligase complex. It is expressed in various tissues with significant occurrence in the brain heart and skeletal muscle. p55/DCAF1 acts as an adaptor that guides substrate proteins to the CUL4-DDB1 complex for ubiquitination influencing protein degradation and homeostasis regulation in the cell.
Functions of p55/DCAF1 extend beyond mere ubiquitination facilitation. It plays a role in transcriptional regulation by interacting with nuclear receptors and histone-modifying enzymes. This protein also contributes to chromatin remodeling hinting its involvement in gene expression control. Involvement in DNA damage response adds to its biological significance connecting it with cellular repair processes. As part of a multiprotein complex p55/DCAF1 cooperates with proteins such as DDB1 in maintaining genomic integrity.
The influence of p55/DCAF1 finds its importance in the cell cycle and DNA repair pathways. In the context of DNA damage response p55/DCAF1 works intricately with other proteins like ATR and CHK1 to monitor and respond to DNA replication stress. Additionally its participative function in the ubiquitin-proteasome pathway links it firmly to broader protein homeostasis networks where it modulates signals involving key regulators such as p53 and beta-catenin.
Researchers have found connections involving p55/DCAF1 in certain cancers and viral infections. Associations with oncogenic pathways suggest that dysregulation of this protein may contribute to tumorigenesis possibly due to its influence on cell cycle control and genomic stability. Furthermore its interaction with viral proteins such as the HIV-1 Vpr has implicated p55/DCAF1 in viral replication and pathogenesis revealing its potential as a therapeutic target in chronic viral diseases.
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