Rabbit Polyclonal P5CS antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ALDH18A1 aa 550 to C-terminus.
View Alternative Names
GSAS, P5CS, PYCS, ALDH18A1, Delta-1-pyrroline-5-carboxylate synthase, Aldehyde dehydrogenase family 18 member A1
- IP
Supplier Data
Immunoprecipitation - Anti-P5CS antibody (AB206682)
Detection of Human P5CS by Western Blot of Immunoprecipitates.
Samples : Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.
Lane 1 : ab206682 used for IP at 6 μg per reaction. For blotting immunoprecipitated P5CS, ab206682 was used at 0.4 μg/mL.
Lane 2 : Control IgG.
Detection : Chemiluminescence with an exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-P5CS antibody (ab206682)
Predicted band size: 87 kDa
false
- WB
Supplier Data
Western blot - Anti-P5CS antibody (AB206682)
Samples prepared using NETN lysis buffer.
All lanes:
Western blot - Anti-P5CS antibody (ab206682) at 0.04 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
293T whole cell lysate at 50 µg
Lane 3:
Jurkat whole cell lysate at 50 µg
Lane 4:
TCMK1 whole cell lysate at 50 µg
Lane 5:
NIH3T3 whole cell lysate at 50 µg
Predicted band size: 87 kDa
true
Exposure time: 10s
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Reactivity data
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P5CS influences cellular responses to oxidative stress due to its role in proline metabolism and redox balance. As part of the proline synthesis pathway P5CS contributes to the production of proline a critical amino acid for protein synthesis. Proline acts as an osmoprotectant and plays a role in cellular structure. P5CS operates within a complex pathway regulating amino acids acting cooperatively with other proteins involved in synthesis processes.
Pathways
P5CS is integral to the proline biosynthetic pathway and is linked to the glutamate pathway. Its activity closely coordinates with the functioning of enzymes like pyrroline-5-carboxylate reductase which converts pyrroline-5-carboxylate to proline. This relationship ensures the continuous supply of proline vital for collagen synthesis and also intersects with pathways involving protein metabolism and cellular stress responses.
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Target data
Publications (1)
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Acta neuropathologica communications 13:197 PubMed40993788
2025
Applications
Unspecified application
Species
Unspecified reactive species
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