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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal P5CS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH18A1 aa 50-250.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Orangutan | Predicted | Predicted | Predicted |
Transfected cell lysate | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Transfected cell lysate | Dilution info 1/0.04000 - 1/0.40000 | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.
Delta-1-pyrroline-5-carboxylate synthase, P5CS, Aldehyde dehydrogenase family 18 member A1, PYCS, P5CS, GSAS, ALDH18A1
Rabbit Polyclonal P5CS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH18A1 aa 50-250.
Delta-1-pyrroline-5-carboxylate synthase, P5CS, Aldehyde dehydrogenase family 18 member A1, PYCS, P5CS, GSAS, ALDH18A1
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
P5CS influences cellular responses to oxidative stress due to its role in proline metabolism and redox balance. As part of the proline synthesis pathway P5CS contributes to the production of proline a critical amino acid for protein synthesis. Proline acts as an osmoprotectant and plays a role in cellular structure. P5CS operates within a complex pathway regulating amino acids acting cooperatively with other proteins involved in synthesis processes.
Pyrroline-5-carboxylate synthetase (P5CS) also known as ALDH18A1 plays an important role in proline biosynthesis. This enzyme catalyzes the conversion of glutamate to pyrroline-5-carboxylate. The P5CS protein has a molecular mass of approximately 87 kDa and is mainly expressed in tissues with high rates of protein synthesis such as the liver and kidney. It has significant expression in the mitochondria reinforcing its function in energy metabolism and amino acid biosynthesis.
P5CS is integral to the proline biosynthetic pathway and is linked to the glutamate pathway. Its activity closely coordinates with the functioning of enzymes like pyrroline-5-carboxylate reductase which converts pyrroline-5-carboxylate to proline. This relationship ensures the continuous supply of proline vital for collagen synthesis and also intersects with pathways involving protein metabolism and cellular stress responses.
Altered P5CS function associates with conditions like cutis laxa and hyperprolinemia. Mutations in the P5CS gene can lead to dysfunction in proline metabolism resulting in elastic skin joint and bone issues and mental retardation in some individuals. The enzyme's interaction with proteins such as the collagen metabolism enzymes highlights its relevance in dermatological and connective tissue disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Paraffin embedded sections of human testis were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
Paraffin embedded sections of human colon were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
Paraffin embedded sections of human cerebral cortex were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
All lanes: Western blot - Anti-P5CS antibody (AB223713) at 1/100 dilution
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 87 kDa
All lanes: Western blot - Anti-P5CS antibody (AB223713) at 1/20 dilution
Lane 1: Caco-2 cell lysate transfected with control siRNA
Lane 2: Caco-2 cell lysate transfected with target specific siRNA probe #1
Lane 3: Caco-2 cell lysate transfected with target specific siRNA probe #2
Predicted band size: 87 kDa
All lanes: Western blot - Anti-P5CS antibody (AB223713) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2: NBT-II (rat bladder tumor cell line) cell lysate
Predicted band size: 87 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P5CS (green) using ab223713 at 4 μg/ml in ICC/IF.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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