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Rabbit Polyclonal P5CS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH18A1 aa 50-250.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ALDH18A1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P54886
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Orangutan
Predicted
Predicted
Predicted
Transfected cell lysate
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/50.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Transfected cell lysate

Dilution info

1/0.04000 - 1/0.40000

Notes

-

Species

Rat

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate

Dilution info

-

Notes

-

Target data

Function

Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.

Alternative names

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Rabbit Polyclonal P5CS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH18A1 aa 50-250.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ALDH18A1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P54886
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

P5CS influences cellular responses to oxidative stress due to its role in proline metabolism and redox balance. As part of the proline synthesis pathway P5CS contributes to the production of proline a critical amino acid for protein synthesis. Proline acts as an osmoprotectant and plays a role in cellular structure. P5CS operates within a complex pathway regulating amino acids acting cooperatively with other proteins involved in synthesis processes.

Activity summary

Pyrroline-5-carboxylate synthetase (P5CS) also known as ALDH18A1 plays an important role in proline biosynthesis. This enzyme catalyzes the conversion of glutamate to pyrroline-5-carboxylate. The P5CS protein has a molecular mass of approximately 87 kDa and is mainly expressed in tissues with high rates of protein synthesis such as the liver and kidney. It has significant expression in the mitochondria reinforcing its function in energy metabolism and amino acid biosynthesis.

Pathways

P5CS is integral to the proline biosynthetic pathway and is linked to the glutamate pathway. Its activity closely coordinates with the functioning of enzymes like pyrroline-5-carboxylate reductase which converts pyrroline-5-carboxylate to proline. This relationship ensures the continuous supply of proline vital for collagen synthesis and also intersects with pathways involving protein metabolism and cellular stress responses.

Associated diseases and disorders

Altered P5CS function associates with conditions like cutis laxa and hyperprolinemia. Mutations in the P5CS gene can lead to dysfunction in proline metabolism resulting in elastic skin joint and bone issues and mental retardation in some individuals. The enzyme's interaction with proteins such as the collagen metabolism enzymes highlights its relevance in dermatological and connective tissue disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713)

    Paraffin embedded sections of human testis were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713)

    Paraffin embedded sections of human colon were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713)

    Paraffin embedded sections of human cerebral cortex were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.

  • Western blot - Anti-P5CS antibody (ab223713), expandable thumbnail

    Western blot - Anti-P5CS antibody (ab223713)

    All lanes: Western blot - Anti-P5CS antibody (AB223713) at 1/100 dilution

    Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate

    Lane 2: U-251 MG (human brain glioma cell line) cell lysate

    Predicted band size: 87 kDa

  • Western blot - Anti-P5CS antibody (ab223713), expandable thumbnail

    Western blot - Anti-P5CS antibody (ab223713)

    All lanes: Western blot - Anti-P5CS antibody (AB223713) at 1/20 dilution

    Lane 1: Caco-2 cell lysate transfected with control siRNA

    Lane 2: Caco-2 cell lysate transfected with target specific siRNA probe #1

    Lane 3: Caco-2 cell lysate transfected with target specific siRNA probe #2

    Predicted band size: 87 kDa

  • Western blot - Anti-P5CS antibody (ab223713), expandable thumbnail

    Western blot - Anti-P5CS antibody (ab223713)

    All lanes: Western blot - Anti-P5CS antibody (AB223713) at 1/100 dilution

    Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Lane 2: NBT-II (rat bladder tumor cell line) cell lysate

    Predicted band size: 87 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-P5CS antibody (ab223713), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-P5CS antibody (ab223713)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P5CS (green) using ab223713 at 4 μg/ml in ICC/IF.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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