JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB223713

Anti-P5CS antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal P5CS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH18A1 aa 50-250.

View Alternative Names

GSAS, P5CS, PYCS, ALDH18A1, Delta-1-pyrroline-5-carboxylate synthase, Aldehyde dehydrogenase family 18 member A1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (AB223713)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (AB223713)

Paraffin embedded sections of human colon were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (AB223713)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (AB223713)

Paraffin embedded sections of human testis were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (AB223713)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (AB223713)

Paraffin embedded sections of human cerebral cortex were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-P5CS antibody (AB223713)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-P5CS antibody (AB223713)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P5CS (green) using ab223713 at 4 μg/ml in ICC/IF.

Western blot - Anti-P5CS antibody (AB223713)
  • WB

Supplier Data

Western blot - Anti-P5CS antibody (AB223713)

All lanes:

Western blot - Anti-P5CS antibody (ab223713) at 1/20 dilution

Lane 1:

Caco-2 cell lysate transfected with control siRNA

Lane 2:

Caco-2 cell lysate transfected with target specific siRNA probe #1

Lane 3:

Caco-2 cell lysate transfected with target specific siRNA probe #2

Predicted band size: 87 kDa

false

Western blot - Anti-P5CS antibody (AB223713)
  • WB

Supplier Data

Western blot - Anti-P5CS antibody (AB223713)

All lanes:

Western blot - Anti-P5CS antibody (ab223713) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 87 kDa

false

Western blot - Anti-P5CS antibody (AB223713)
  • WB

Supplier Data

Western blot - Anti-P5CS antibody (AB223713)

All lanes:

Western blot - Anti-P5CS antibody (ab223713) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat bladder tumor cell line) cell lysate

Predicted band size: 87 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ALDH18A1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

P54886

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/0.04 - 1/0.4", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pyrroline-5-carboxylate synthetase (P5CS) also known as ALDH18A1 plays an important role in proline biosynthesis. This enzyme catalyzes the conversion of glutamate to pyrroline-5-carboxylate. The P5CS protein has a molecular mass of approximately 87 kDa and is mainly expressed in tissues with high rates of protein synthesis such as the liver and kidney. It has significant expression in the mitochondria reinforcing its function in energy metabolism and amino acid biosynthesis.
Biological function summary

P5CS influences cellular responses to oxidative stress due to its role in proline metabolism and redox balance. As part of the proline synthesis pathway P5CS contributes to the production of proline a critical amino acid for protein synthesis. Proline acts as an osmoprotectant and plays a role in cellular structure. P5CS operates within a complex pathway regulating amino acids acting cooperatively with other proteins involved in synthesis processes.

Pathways

P5CS is integral to the proline biosynthetic pathway and is linked to the glutamate pathway. Its activity closely coordinates with the functioning of enzymes like pyrroline-5-carboxylate reductase which converts pyrroline-5-carboxylate to proline. This relationship ensures the continuous supply of proline vital for collagen synthesis and also intersects with pathways involving protein metabolism and cellular stress responses.

Altered P5CS function associates with conditions like cutis laxa and hyperprolinemia. Mutations in the P5CS gene can lead to dysfunction in proline metabolism resulting in elastic skin joint and bone issues and mental retardation in some individuals. The enzyme's interaction with proteins such as the collagen metabolism enzymes highlights its relevance in dermatological and connective tissue disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.
See full target information ALDH18A1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 24:102 PubMed40165182

2025

C1Q TPP1 macrophages promote colon cancer progression through SETD8-driven p53 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Veschi,Francesco Verona,Sebastiano Di Bella,Alice Turdo,Miriam Gaggianesi,Simone Di Franco,Laura Rosa Mangiapane,Chiara Modica,Melania Lo Iacono,Paola Bianca,Ornella Roberta Brancato,Caterina D'Accardo,Gaetana Porcelli,Vincenzo Luca Lentini,Isabella Sperduti,Elisabetta Sciacca,Peter Fitzgerald,David Lopez-Perez,Pierre Martine,Kate Brown,Giuseppe Giannini,Ettore Appella,Giorgio Stassi,Matilde Todaro

JCI insight 9: PubMed39315548

2024

Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Contento,Jo-Anne Am Wilson,Brintha Selvarajah,Manuela Platé,Delphine Guillotin,Valle Morales,Marcello Trevisani,Vanessa Pitozzi,Katiuscia Bianchi,Rachel C Chambers
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB206682

Anti-P5CS antibody

Western blot - Anti-P5CS antibody (AB206682)

  • 0
  • 0
Primary Antibodies

AB225999

Anti-P5CS antibody

Immunoprecipitation - Anti-P5CS antibody (AB225999)

  • 0
  • 0

Complementary Products

Assay Kits

AB303731

Proline Assay Kit (Fluorometric)

Functional Studies - Proline Assay Kit (Fluorometric) (AB303731)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB137679

Anti-ornithine aminotransferase antibody

Immunocytochemistry/ Immunofluorescence - Anti-ornithine aminotransferase antibody (AB137679)

  • 5
  • 1
Primary Antibodies

AB168352

Anti-GLUD1 + GLUD2 antibody [EPR11370]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

  • 3
  • 1
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com