AB109442

Anti-p60 CAF1/MPP7 antibody [EPR6105]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Recombinant Monoclonal p60 CAF1/MPP7 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

View Alternative Names

CAF1A, CAF1P60, MPHOSPH7, MPP7, CHAF1B, Chromatin assembly factor 1 subunit B, CAF-1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7, CAF-I 60 kDa subunit, CAF-I p60

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Lab

Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

p60 CAF1/MPP7 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with 109442 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab109442 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab109442 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Predicted band size: 61 kDa

Observed band size: 70 kDa

false

Unknown

Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

All lanes:

Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Predicted band size: 61 kDa

Observed band size: 70 kDa

false

Carrier free

Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6105

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target p60 CAF1/MPP7 also known simply as MPP7 is a protein involved in cellular mechanisms regarding chromatin assembly. It has a mass of approximately 55 kDa. Expression of MPP7 occurs predominantly in the cytoplasm and nucleus. Its function involves association with chromatin assembly factor 1 (CAF-1) complex hence the name p60 CAF1 highlighting its role in DNA replication and repair processes.

Biological function summary

In cellular environments MPP7 is significant in maintaining cell polarity and epithelial cell junctions. It plays an important part in forming the Crumbs-3 (CRB3) complex an essential component regulating epithelial cell polarity. Through this complex MPP7 collaborates closely with PATJ (Pals1-associated tight junction protein) and DLG1 (Discs Large Homolog 1) contributing to cell junction stability and signal transduction pathways that regulate epithelial integrity.

Pathways

MPP7 is integral to epithelial cell polarity pathways and cell adhesion signaling. It affects pathway activities by coordinating with proteins like DLG1 and PATJ. By aligning with the CRB3 complex MPP7 influences processes within the Hippo signaling pathway a pathway that controls cell proliferation and apoptosis. This positions MPP7 as a mediator balancing cell growth and communication in complex signaling networks.

MPP7 links to cancer progression and epithelial tissue-related conditions. Due to its role in maintaining cell polarity and structure alterations or disruptions in MPP7 functions could contribute to tumorigenesis. This connection emerges through interactions with PATJ and DLG1 impacting the integrity of epithelial junctions also potentially influencing conditions like Inflammatory Bowel Disease (IBD) where epithelial barrier function is important. Research continues to elucidate the exact mechanisms opening paths for therapeutic targeting in these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

See full target information CHAF1B

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37129573

2023

The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability.

Applications

Unspecified application

Species

Unspecified reactive species

Roshan L Shrestha,Vinutha Balachandra,Jee Hun Kim,Austin Rossi,Pranathi Vadlamani,Subhash Chandra Sethi,Laurent Ozbun,Shinjen Lin,Ken Chin-Chien Cheng,Raj Chari,Tatiana S Karpova,Gianluca Pegoraro,Daniel R Foltz,Natasha J Caplen,Munira A Basrai

Proceedings of the National Academy of Sciences of the United States of America 119: PubMed35074917

2022

CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska K Geis,Yosef Sabo,Xiao Chen,Yinglu Li,Chao Lu,Stephen P Goff

Frontiers in medicine 8:654635 PubMed34109194

2021

Multi-Omics Integrative Analysis Uncovers Molecular Subtypes and mRNAs as Therapeutic Targets for Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Shen,Wei Xiong,Qi Gu,Qin Zhang,Jia Yue,Changsong Liu,Duan Wang

eLife 10: PubMed33970102

2021

Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Tachiwana,Mariko Dacher,Kazumitsu Maehara,Akihito Harada,Yosuke Seto,Ryohei Katayama,Yasuyuki Ohkawa,Hiroshi Kimura,Hitoshi Kurumizaka,Noriko Saitoh

Oncology letters 20:2338-2346 PubMed32782551

2020

CAF-1/p150 promotes cell proliferation, migration, invasion and predicts a poor prognosis in patients with cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Yang,Qian Long,Min Chen,Xiao Liu,Hang Zhou

Oncology reports 41:2518-2528 PubMed30720130

2019

CHAF1B promotes proliferation and reduces apoptosis in 95‑D lung cancer cells and predicts a poor prognosis in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Duan,Tanzhen Liu,Shengwen Li,Mingguang Huang,Xiaoyu Li,Hui Zhao,Jianqiang Li

Molecular cell 72:888-901.e7 PubMed30344095

2018

The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Piquet,Florent Le Parc,Siau-Kun Bai,Odile Chevallier,Salomé Adam,Sophie E Polo

Molecular cell 71:11-24.e7 PubMed29937341

2018

ATRX Promotes DNA Repair Synthesis and Sister Chromatid Exchange during Homologous Recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Szilvia Juhász,Amira Elbakry,Arthur Mathes,Markus Löbrich

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p60 CAF1/MPP7 antibody [EPR6105]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (AB109442)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise