Rabbit Recombinant Monoclonal p60 CAF1/MPP7 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--western-blot-img116545.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)")
![Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--flow-cytometry-intracellular-img139279.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)")
![Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--immunoprecipitation-img171748.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Alternative names
Chromatin assembly factor 1 subunit B, CAF-1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7, CAF-I 60 kDa subunit, CAF-I p60, CAF1P60, MPHOSPH7, CAF1A, CHAF1B, MPP7
Recommended products
Rabbit Recombinant Monoclonal p60 CAF1/MPP7 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR6105
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226065 is the carrier-free version of Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target p60 CAF1/MPP7 also known simply as MPP7 is a protein involved in cellular mechanisms regarding chromatin assembly. It has a mass of approximately 55 kDa. Expression of MPP7 occurs predominantly in the cytoplasm and nucleus. Its function involves association with chromatin assembly factor 1 (CAF-1) complex hence the name p60 CAF1 highlighting its role in DNA replication and repair processes.
- Biological function summary
In cellular environments MPP7 is significant in maintaining cell polarity and epithelial cell junctions. It plays an important part in forming the Crumbs-3 (CRB3) complex an essential component regulating epithelial cell polarity. Through this complex MPP7 collaborates closely with PATJ (Pals1-associated tight junction protein) and DLG1 (Discs Large Homolog 1) contributing to cell junction stability and signal transduction pathways that regulate epithelial integrity.
- Pathways
MPP7 is integral to epithelial cell polarity pathways and cell adhesion signaling. It affects pathway activities by coordinating with proteins like DLG1 and PATJ. By aligning with the CRB3 complex MPP7 influences processes within the Hippo signaling pathway a pathway that controls cell proliferation and apoptosis. This positions MPP7 as a mediator balancing cell growth and communication in complex signaling networks.
- Associated diseases and disorders
MPP7 links to cancer progression and epithelial tissue-related conditions. Due to its role in maintaining cell polarity and structure alterations or disruptions in MPP7 functions could contribute to tumorigenesis. This connection emerges through interactions with PATJ and DLG1 impacting the integrity of epithelial junctions also potentially influencing conditions like Inflammatory Bowel Disease (IBD) where epithelial barrier function is important. Research continues to elucidate the exact mechanisms opening paths for therapeutic targeting in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--western-blot-img116545.jpg "Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)")
Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)
This data was developed using Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442)
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: Caco-2 cell lysate at 10 µg
Predicted band size: 61 kDa
Observed band size: 70 kDa
![Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--flow-cytometry-intracellular-img139279.jpg "Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)")
Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)
This data was developed using Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--immunoprecipitation-img171748.jpg "Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)")
Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)
This data was developed using Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442, the same antibody clone in a different buffer formulation.p60 CAF1/MPP7 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: abab109442 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442)
Predicted band size: 61 kDa
Observed band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136631.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)
This data was developed using Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442, the same antibody clone in a different buffer formulation.Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065), expandable thumbnail](https://content.abcam.com/products/images/p60-caf1-mpp7-antibody-epr6105-bsa-and-azide-free-ab226065--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137786.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)
This data was developed using Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442, the same antibody clone in a different buffer formulation.Anti-p60 CAF1/MPP7 antibody [EPR6105] ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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