Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation.

ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation.

ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• IP

Lab

Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation. p60 CAF1/MPP7 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with 109442 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab109442 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab109442 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (<a href='/en-us/products/primary-antibodies/p60-caf1-mpp7-antibody-epr6105-ab109442'>ab109442</a>)

Predicted band size: 61 kDa

Observed band size: 70 kDa

false

• WB

Unknown

Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (AB226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (<a href='/en-us/products/primary-antibodies/p60-caf1-mpp7-antibody-epr6105-ab109442'>ab109442</a>)

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Predicted band size: 61 kDa

Observed band size: 70 kDa

false