AB232891

Anti-p60 katanin antibody

Name: Anti-p60 katanin antibody (ab232891) | Abcam
Brand: Abcam Limited
SKU: AB232891
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal p60 katanin antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Human, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KATNA1 aa 1-250.

View Alternative Names

Katanin p60 ATPase-containing subunit A1, Katanin p60 subunit A1, p60 katanin, KATNA1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 katanin antibody (AB232891)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 katanin antibody (AB232891)

Formalin-fixed, paraffin-embedded human kidney tissue stained for p60 katanin using ab232891 at 10 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 katanin antibody (AB232891)

Formalin-fixed, paraffin-embedded human stomach tissue stained for p60 katanin using ab232891 at 10 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 katanin antibody (AB232891)

Formalin-fixed, paraffin-embedded human liver tissue stained for p60 katanin using ab232891 at 10 μg/ml in immunohistochemical analysis.

Supplier Data

Western blot - Anti-p60 katanin antibody (AB232891)

All lanes:

Western blot - Anti-p60 katanin antibody (ab232891) at 1 µg/mL

All lanes:

Recombinant human p60 katanin protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 56 kDa

true

Supplier Data

Western blot - Anti-p60 katanin antibody (AB232891)

All lanes:

Western blot - Anti-p60 katanin antibody (ab232891) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Pig stomach tissue lysate

Lane 3:

Pig brain tissue lysate

Lane 4:

Pig spleen tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 56 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human KATNA1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O75449

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P60 katanin sometimes called katanin-p60 or KATNA1 is an important protein involved in microtubule severing. The protein has a molecular mass of approximately 60 kDa. It is expressed in various tissues with a high presence in the nervous system. Mechanically p60 katanin disrupts microtubules by hydrolyzing ATP which results in microtubule fragmentation. This activity requires the proper interaction of its two subunits p60 and p80 to execute its full function.

Biological function summary

P60 katanin plays a significant role in cellular processes such as mitosis where it regulates microtubule dynamics. It is part of a larger complex that includes the regulatory subunit p80 katanin which assists in targeting and anchoring. p60 katanin's activity influences the organization of the microtubule network which is vital for intracellular transport and cell division especially in neurons where it affects axon growth and pruning.

Pathways

P60 katanin is involved in the mitotic and neuronal development pathways. It interacts with proteins like spastin and katanin-like proteins within these pathways. In the mitotic pathway it facilitates chromosome segregation and mitotic spindle organization. During neuronal development its microtubule-severing action contributes to remodeling the cytoskeleton therefore aiding in neuron differentiation and migration.

Abnormal p60 katanin activity links to neurological conditions such as Alzheimer's disease and spastic paraplegia. Mutations or dysregulation in p60 katanin or its pathway associates can lead to impaired microtubule dynamics influencing these diseases. In spastic paraplegia p60 katanin's interaction with spastin is particularly notable as both proteins are involved in microtubule maintenance and defects in either can contribute to the disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.

See full target information KATNA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 20:244 PubMed32549791

2020

Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangzong Chen,Chunchun Bao,Xiuxing Zhang,Xinshi Lin,Yimou Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p60 katanin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 katanin antibody (AB232891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 katanin antibody (AB232891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 katanin antibody (AB232891)

Western blot - Anti-p60 katanin antibody (AB232891)

Western blot - Anti-p60 katanin antibody (AB232891)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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