AB111881

Anti-p60 katanin antibody [EPR5071]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal p60 katanin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Katanin p60 ATPase-containing subunit A1, Katanin p60 subunit A1, p60 katanin, KATNA1

2 Images

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Western blot - Anti-p60 katanin antibody [EPR5071] (AB111881)

All lanes:

Western blot - Anti-p60 katanin antibody [EPR5071] (ab111881) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-p60 katanin antibody [EPR5071] (AB111881)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p60 katanin antibody [EPR5071] (AB111881)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling p60 katanin with ab111881 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing staining on the centrosome of Hela cells.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab111881 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Carrier free

Anti-p60 katanin antibody [EPR5071] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5071

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P60 katanin sometimes called katanin-p60 or KATNA1 is an important protein involved in microtubule severing. The protein has a molecular mass of approximately 60 kDa. It is expressed in various tissues with a high presence in the nervous system. Mechanically p60 katanin disrupts microtubules by hydrolyzing ATP which results in microtubule fragmentation. This activity requires the proper interaction of its two subunits p60 and p80 to execute its full function.

Biological function summary

P60 katanin plays a significant role in cellular processes such as mitosis where it regulates microtubule dynamics. It is part of a larger complex that includes the regulatory subunit p80 katanin which assists in targeting and anchoring. p60 katanin's activity influences the organization of the microtubule network which is vital for intracellular transport and cell division especially in neurons where it affects axon growth and pruning.

Pathways

P60 katanin is involved in the mitotic and neuronal development pathways. It interacts with proteins like spastin and katanin-like proteins within these pathways. In the mitotic pathway it facilitates chromosome segregation and mitotic spindle organization. During neuronal development its microtubule-severing action contributes to remodeling the cytoskeleton therefore aiding in neuron differentiation and migration.

Abnormal p60 katanin activity links to neurological conditions such as Alzheimer's disease and spastic paraplegia. Mutations or dysregulation in p60 katanin or its pathway associates can lead to impaired microtubule dynamics influencing these diseases. In spastic paraplegia p60 katanin's interaction with spastin is particularly notable as both proteins are involved in microtubule maintenance and defects in either can contribute to the disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.

See full target information KATNA1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36107470

2022

Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.

Applications

Unspecified application

Species

Unspecified reactive species

Dawn M Wenzel,Douglas R Mackay,Jack J Skalicky,Elliott L Paine,Matthew S Miller,Katharine S Ullman,Wesley I Sundquist

Cancer biology & therapy 20:1337-1347 PubMed31345098

2019

Reorganization of paclitaxel-stabilized microtubule arrays at mitotic entry: roles of depolymerizing kinesins and severing proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica C Leung,Lynne Cassimeris

PLoS biology 17:e3000381 PubMed31314751

2019

Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.

Applications

Unspecified application

Species

Unspecified reactive species

Mary Mirvis,Kathleen A Siemers,W James Nelson,Tim P Stearns

Oncology letters 15:4963-4969 PubMed29552132

2018

The role of katanin p60 in breast cancer bone metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenrong Fu,Hui Wu,Zhengjiang Cheng,Shaojun Huang,Hui Rao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p60 katanin antibody [EPR5071]

Western blot - Anti-p60 katanin antibody [EPR5071] (AB111881)

Immunocytochemistry/ Immunofluorescence - Anti-p60 katanin antibody [EPR5071] (AB111881)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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