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AB735

Anti-p63 antibody [4A4]

5

(12 Reviews)

|

(203 Publications)

Anti-p63 antibody [4A4] (ab735) is a mouse monoclonal antibody detecting p63 in IHC-P. Suitable for Human.

- Over 150 publications
- Trusted since 2002

View Alternative Names

KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)

Immunohistochemical analysis of human prostate tissue labelling p63 with ab735.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)
  • IHC-P

CiteAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)

p63 Immunohistochemistry using Anti-p63 antibody [4A4] ab735. Publication image and figure legend from Kang, H. N., Kim, J. H., et al., 2020, BMC Cancer, PubMed 32293356.

Comparison of histopathologic and immunohistochemistry findings between patients and their matched patient-derived xenografts (PDXs, F2 generation) of head and neck cancer squamous cell carcinoma (HNSCC). Left columns show hematoxylin and eosin-staining and right columns show immunohistochemical staining of p63, a marker of squamous cell carcinoma, from patients and the PDX of each model. Representative stained sections are shown (magnification : 200x in patient samples; scale bars = 100 μm)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)
  • IHC-P

CiteAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)

p63 immunohistochemistry using Anti-p63 antibody [4A4] ab735. Publication image and figure legend from Wang, H., Yuan, Q., et al. 2018, Cell Death Dis, PubMed 29362488.

The phenotypic characterization of the P63(+/-) mice and wild-type miceThe images of testes from wild-type mice a and P63(+/-) mice b at 2 months old. The testis weight of wild-type mice and P63(+/-) mice at 2 months old (n = 6) c. H&E staining of testis sections from wild-type mice (d, left panel) and P63(+/-) mice (d, right panel) at 2 months old. Scale bar in d = 20 μm. The numbers of pups per litter of wild-type mice and P63(+/-) male mice at 2-8 months old crossed with wild-type female mice e. The percentages of motile sperm f and progressive sperm g in wild-type mice and P63(+/-) mice at 2 months old. All data of motility measurements were presented from at least three independent experiments. The fecundity of P63(+/-) mice and wild-type mice at round 12 months f. The morphology of sperm of wild-type mice i and P63(+/-) mice j at 2 months old under a phase-contrast microscope. H&E staining of sperm from wild-type mice k and P63(+/-) mice l at 2 months old. Scale bars in i-l = 20 μm

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4A4

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Tp63 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O88898

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>See Sanderson et al and Huange et al. or the Abreview 22860.</p><p>Alternatively, steam tissue sections for 45-60 minutes. Allow solution to cool for 10 minutes then wash in distilled water.</p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol." }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Please note that clone [BC4A4] and clone [4A4] are identical.

This product was changed from ascites to tissue culture supernatant on 14/07/17. The following lots are from ascites and are still in stock as of 14/07/17 – GR323232, GR293168, and GR301030. Lot numbers higher than GR323232 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Product Specifications
Anti-p63 antibody [4A4] (ab735) is a mouse monoclonal antibody and is validated for use in IHC-P in human samples.
Anti-p63 antibody [4A4] (ab735) specifically detects p63 (UniProt ID: Q9H3D4; Molecular weight: 77kDa) and is sold in 250 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-p63 antibody [4A4] (ab735) has high sensitivity and specificity.
Anti-p63 antibody [4A4] (ab735) has been cited over 153 times in peer reviewed journals and is trusted by the scientific community.
Anti-p63 antibody [4A4] (ab735) has 12 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone 4A4 - ab256584.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Protein A/G affinity column
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p63 protein also known as TP63 or KET is a transcription factor that plays a role in the regulation of cell cycle and apoptosis. With a molecular mass of approximately 77 kDa it expresses predominantly in epithelial tissues such as those in the skin breast and prostate. The expression pattern of p63 makes it a useful marker in developmental biology and cancer diagnosis. Antibodies like '4A4' specifically recognize p63 in various assays including immunohistochemistry.
Biological function summary

P63 participates in the control of epithelial cell differentiation and proliferation. It functions as a member of the p53 family which includes p53 and p73. This family forms an important part of the complex regulatory network that maintains genomic stability. Research using methods like p63 staining in immunohistochemistry highlights its critical role in stratified epithelia. The gene encodes several isoforms with different biological activities impacting processes such as cellular senescence and development.

Pathways

P63 integrates into various signaling pathways that influence cellular growth and adherence. It notably impacts the Notch and Wnt pathways both of which are critical for developmental processes and cancer progression. Through these pathways p63 interacts with proteins like β-catenin and Notch receptors facilitating cross-talk that influences cell fate decisions. Its function relates closely to other transcription factors forming feedback loops that reinforce its regulatory impact.

P63 shows strong associations with ectodermal dysplasia and different types of cancers like squamous cell carcinoma. Mutations and altered expressions of p63 contribute to these pathologies by disrupting normal cell proliferation and differentiation. The protein interacts closely with p73 in cancer suggesting a compensatory role when p63 function is compromised. Understanding these connections can guide the development of targeted therapies and improve diagnostic accuracy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
See full target information TP63
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Publications (203)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30620 PubMed40921827

2025

MDM4 enables efficient human iPS cell generation from PBMCs using synthetic RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Masato Nakagawa,Mizuho Nogi,Hatsuki Doi,Ryuhei Hayashi,Tomohiko Katayama,Hirohisa Ohno,Megumi Mochizuki,Karin Hayashi,Hirohide Saito

JID innovations : skin science from molecules to population health 5:100394 PubMed40786655

2025

Identification of Keratin 5-Expressing Fibroblasts for Regenerating Keratinocytes in the Necrotic Skin Graft.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshikazu Hirose,Asaka Miura,Yuki Kobayashi,Takashi Shimbo,Yuya Ouchi,Tomomi Kitayama,Tomoya Mori,Akio Tanaka,Manabu Fujimoto,Kotaro Saga,Katsuto Tamai

STAR protocols 6:103892 PubMed40531627

2025

Protocols to co-culture human primary lung cells in the simple-flow device.

Applications

Unspecified application

Species

Unspecified reactive species

Cinta Iriondo,Sem Koornneef,Kari-Pekka Skarp,Marjon Buscop-van Kempen,Anne Boerema-de Munck,Robbert J Rottier

Stem cell research & therapy 16:296 PubMed40483513

2025

KRT5 TP63-expressing urothelial basal cells act as a driver to bladder urothelium regeneration in rabbit.

Applications

Unspecified application

Species

Unspecified reactive species

Jiasheng Chen,Mingming Yu,Lin Wang,Hua Xie,Yiqing Lv,Yichen Huang,Yue Hong,Fang Chen

International wound journal 22:e70033 PubMed40320827

2025

Secretome from prolonged high-density human Wharton's jelly stem cell culture accelerates wound healing in both in vitro and in vivo models.

Applications

Unspecified application

Species

Unspecified reactive species

Jiah Shin Chin,Mandy Li Ling Tan,Priscilla Lay Keng Lim,Bhavya Sharma,Aimin Yeo,Yi Bing Aw,Yi Zhen Ng,Carine Bonnard,David L Becker,Pamela Mok

PloS one 20:e0321579 PubMed40257992

2025

Extracellular vesicles from adipose-derived mesenchymal stem cells promote colony formation ability and EMT of corneal limbal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqin Li,Ryuhei Hayashi,Tsutomu Imaizumi,Jodie Harrington,Yuji Kudo,Hiroshi Takayanagi,Koichi Baba,Kohji Nishida

Virology journal 22:99 PubMed40234888

2025

Long-term culture of chicken tracheal organoids for the purpose of avian influenza virus research.

Applications

Unspecified application

Species

Unspecified reactive species

Anja C M de Bruin,Mart M Lamers,Bart L Haagmans,Lonneke M Leijten,Ron A M Fouchier,Mathilde Richard

Stem cell reports 20:102447 PubMed40086446

2025

Differentiation success of reprogrammed cells is heterogeneous in vivo and modulated by somatic cell identity memory.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Zikmund,Jonathan Fiorentino,Chris Penfold,Marco Stock,Polina Shpudeiko,Gaurav Agarwal,Larissa Langfeld,Kseniya Petrova,Leonid Peshkin,Stephan Hamperl,Antonio Scialdone,Eva Hoermanseder

Nature communications 16:1303 PubMed39900892

2025

Cloned airway basal progenitor cells to repair fibrotic lung through re-epithelialization.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhao,Yueqing Zhou,Weipan Zhang,Mingzhe Liu,Jun Duan,Xiaopeng Zhang,Qiwang Ma,Yujia Wang,Yuzhen Zhang,Zhongliang Guo,Ting Zhang,Wei Zuo

Oncology letters 29:107 PubMed39736923

2024

Primary tracheal hyalinizing clear cell carcinoma with EWSR1 rearrangement: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Wenkang Zong,Guozheng Gao,Xike Lu,Daqiang Sun
View all publications
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Product promise

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