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AB110038

Anti-p63 antibody [4E5]

4

(4 Reviews)

|

(4 Publications)

Mouse Monoclonal P63 antibody. Suitable for Flow Cyt, ELISA, WB and reacts with Human, Mouse, African green monkey, Rat, Synthetic peptide samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TP63 aa 650 to C-terminus.

View Alternative Names

KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L

3 Images
Flow Cytometry - Anti-p63 antibody [4E5] (AB110038)
  • Flow Cyt

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Flow Cytometry - Anti-p63 antibody [4E5] (AB110038)

Overlay histogram showing A431 cells stained with ab110038 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110038, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-p63 antibody [4E5] (AB110038)
  • WB

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Western blot - Anti-p63 antibody [4E5] (AB110038)

All lanes:

Western blot - Anti-p63 antibody [4E5] (ab110038) at 1/500 dilution

Lane 1:

A431 cell lysate.

Lane 2:

HeLa cell lysate.

Lane 3:

Jurkat cell lysate.

Lane 4:

THP1 cell lysate.

Lane 5:

NIH 3T3 cell lysate.

Lane 6:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate.

Lane 7:

PC12 cell lysate.

Predicted band size: 77 kDa

Observed band size: 51 kDa

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ELISA - Anti-p63 antibody [4E5] (AB110038)
  • ELISA

Unknown

ELISA - Anti-p63 antibody [4E5] (AB110038)

ab110038 should be used at a proposed dilution of 1/10000 for ELISA.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4E5

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human, African green monkey

Applications

WB, ELISA, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human TP63 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H3D4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR118984-25 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p63 protein also known as TP63 or KET is a transcription factor that plays a role in the regulation of cell cycle and apoptosis. With a molecular mass of approximately 77 kDa it expresses predominantly in epithelial tissues such as those in the skin breast and prostate. The expression pattern of p63 makes it a useful marker in developmental biology and cancer diagnosis. Antibodies like '4A4' specifically recognize p63 in various assays including immunohistochemistry.
Biological function summary

P63 participates in the control of epithelial cell differentiation and proliferation. It functions as a member of the p53 family which includes p53 and p73. This family forms an important part of the complex regulatory network that maintains genomic stability. Research using methods like p63 staining in immunohistochemistry highlights its critical role in stratified epithelia. The gene encodes several isoforms with different biological activities impacting processes such as cellular senescence and development.

Pathways

P63 integrates into various signaling pathways that influence cellular growth and adherence. It notably impacts the Notch and Wnt pathways both of which are critical for developmental processes and cancer progression. Through these pathways p63 interacts with proteins like β-catenin and Notch receptors facilitating cross-talk that influences cell fate decisions. Its function relates closely to other transcription factors forming feedback loops that reinforce its regulatory impact.

P63 shows strong associations with ectodermal dysplasia and different types of cancers like squamous cell carcinoma. Mutations and altered expressions of p63 contribute to these pathologies by disrupting normal cell proliferation and differentiation. The protein interacts closely with p73 in cancer suggesting a compensatory role when p63 function is compromised. Understanding these connections can guide the development of targeted therapies and improve diagnostic accuracy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
See full target information TP63
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell journal 25:372-382 PubMed37434454

2023

Decellularized Lung Extracellular Matrix Scaffold Promotes Human Embryonic Stem Cell Differentiation towards Alveolar Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Afshin Noori,Mohammad Reza Mokhber Dezfouli,Sareh Rajabi,Fatemeh Ganji,Zahra Ghezelayagh,Elie El Agha,Hossein Baharvand,Sirous Sadeghian Chaleshtori,Yaser Tahamtani

Cellular & molecular immunology : PubMed31900457

2020

CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo Chen,Ke Wang,Zhuan Feng,Ming-Yang Zhang,Jiao Wu,Jie-Jie Geng,Zhi-Nan Chen

Cell cycle (Georgetown, Tex.) 15:1439-49 PubMed27096226

2016

Deoxycholic acid (DCA) confers an intestinal phenotype on esophageal squamous epithelium via induction of the stemness-associated reprogramming factors OCT4 and SOX2.

Applications

Unspecified application

Species

Unspecified reactive species

Caifei Shen,Haoxiang Zhang,Pu Wang,Ji Feng,Jingwen Li,Yin Xu,Anran Zhang,Shunzi Shao,Xiaona Yu,Wu Yan,Yiju Xia,Jiali Hu,Dianchun Fang

Nature 486:266-70 PubMed22699621

2012

The deubiquitinase USP9X suppresses pancreatic ductal adenocarcinoma.

Applications

WB

Species

Unspecified reactive species

Pedro A Pérez-Mancera,Alistair G Rust,Louise van der Weyden,Glen Kristiansen,Allen Li,Aaron L Sarver,Kevin A T Silverstein,Robert Grützmann,Daniela Aust,Petra Rümmele,Thomas Knösel,Colin Herd,Derek L Stemple,Ross Kettleborough,Jacqueline A Brosnan,Ang Li,Richard Morgan,Spencer Knight,Jun Yu,Shane Stegeman,Lara S Collier,Jelle J ten Hoeve,Jeroen de Ridder,Alison P Klein,Michael Goggins,Ralph H Hruban,David K Chang,Andrew V Biankin,Sean M Grimmond,Lodewyk F A Wessels,Stephen A Wood,Christine A Iacobuzio-Donahue,Christian Pilarsky,David A Largaespada,David J Adams,David A Tuveson
View all publications
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