Rabbit Polyclonal P63 antibody. Suitable for WB, ICC/IF, IP and reacts with Human, Rat, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TP63 aa 100-400.
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- Nature 589:448-4552020Fat1 deletion promotes hybrid EMT state, tumour stemness and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIevgenia Pastushenko,Federico Mauri,Yura Song,Florian de Cock,Bob Meeusen,Benjamin Swedlund,Francis Impens,Delphi Van Haver,Matthieu Opitz,Manuel Thery,Yacine Bareche,Gaelle Lapouge,Marjorie Vermeersch,Yves-Rémi Van Eycke,Cédric Balsat,Christine Decaestecker,Youri Sokolow,Sergio Hassid,Alicia Perez-Bustillo,Beatriz Agreda-Moreno,Luis Rios-Buceta,Pedro Jaen,Pedro Redondo,Ramon Sieira-Gil,Jose F Millan-Cayetano,Onofre Sanmatrtin,Nicky D'Haene,Virginie Moers,Milena Rozzi,Jeremy Blondeau,Sophie Lemaire,Samuel Scozzaro,Veerle Janssens,Magdalena De Troya,Christine Dubois,David Pérez-Morga,Isabelle Salmon,Christos Sotiriou,Francoise Helmbacher,Cédric BlanpainPubMed 33328637
- Stem cells and development 29:1365-13692020Human Induced Pluripotent Stem Cell-Derived Lung Epithelial System for SARS-CoV-2 Infection Modeling and Its Potential in Drug Repurposing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHarshini Surendran et. al.PubMed 32867617
- British journal of cancer 115:983-9922016p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma.
- Development (Cambridge, England) 143:2920-92016Fgf signalling controls diverse aspects of fin regeneration.Applications:IHCReactive species:ZebrafishEri Shibata,Yuki Yokota,Natsumi Horita,Akira Kudo,Gembu Abe,Koichi Kawakami,Atsushi KawakamiPubMed 27402707
- Oncotarget 7:28836-482016Setdb1, a novel interactor of ΔNp63, is involved in breast tumorigenesis.Applications:WBReactive species:Unspecified reactive speciesCarla Regina,Mirco Compagnone,Angelo Peschiaroli,AnnaMaria Lena,Margherita Annicchiarico-Petruzzelli,Maria Cristina Piro,Gerry Melino,Eleonora CandiPubMed 26840455
- PloS one 10:e01224932015Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.Applications:I-ELISAReactive species:Unspecified reactive speciesAna Mafalda Baptista Tadeu et. al.PubMed 25849374
- Development (Cambridge, England) 140:3777-862013Notch signaling represses p63 expression in the developing surface ectoderm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Mafalda Baptista Tadeu et. al.PubMed 23924630
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TP63 aa 100-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H3D4
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Reactivity data
Select an application| WB | ICC/IF | IP | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
Alternative names
KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L
Recommended products
Rabbit Polyclonal P63 antibody. Suitable for WB, ICC/IF, IP and reacts with Human, Rat, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TP63 aa 100-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TP63 aa 100-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H3D4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p63 protein also known as TP63 or KET is a transcription factor that plays a role in the regulation of cell cycle and apoptosis. With a molecular mass of approximately 77 kDa it expresses predominantly in epithelial tissues such as those in the skin breast and prostate. The expression pattern of p63 makes it a useful marker in developmental biology and cancer diagnosis. Antibodies like '4A4' specifically recognize p63 in various assays including immunohistochemistry.
- Biological function summary
P63 participates in the control of epithelial cell differentiation and proliferation. It functions as a member of the p53 family which includes p53 and p73. This family forms an important part of the complex regulatory network that maintains genomic stability. Research using methods like p63 staining in immunohistochemistry highlights its critical role in stratified epithelia. The gene encodes several isoforms with different biological activities impacting processes such as cellular senescence and development.
- Pathways
P63 integrates into various signaling pathways that influence cellular growth and adherence. It notably impacts the Notch and Wnt pathways both of which are critical for developmental processes and cancer progression. Through these pathways p63 interacts with proteins like β-catenin and Notch receptors facilitating cross-talk that influences cell fate decisions. Its function relates closely to other transcription factors forming feedback loops that reinforce its regulatory impact.
- Associated diseases and disorders
P63 shows strong associations with ectodermal dysplasia and different types of cancers like squamous cell carcinoma. Mutations and altered expressions of p63 contribute to these pathologies by disrupting normal cell proliferation and differentiation. The protein interacts closely with p73 in cancer suggesting a compensatory role when p63 function is compromised. Understanding these connections can guide the development of targeted therapies and improve diagnostic accuracy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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9 product images
Western blot - Anti-p63 antibody (ab97865)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-p63 antibody (ab97865) at 1/500 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 77 kDa
Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody (ab97865)
ab97865 at 1/200 dilution staining p63 in A431 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
Western blot - Anti-p63 antibody (ab97865)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-p63 antibody (ab97865) at 1/500 dilution
All lanes: Rat brain lysates at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 76 kDa
Western blot - Anti-p63 antibody (ab97865)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-p63 antibody (ab97865) at 1/2000 dilution
Lane 1: Non-transfected (–) A431 whole cell extracts at 30 µg
Lane 2: Transfected (+) A431 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 76 kDa
Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody (ab97865)
Immunofluorescence analysis of 4% paraformaldehyde fixed A431 cells labelling p63 protein at nucleus using ab97865 at a 1/500 dilution at RT for 15 min (Green). alpha Tubulin, a cytoskeleton marker was stained with anti-alpha Tubulin antibody at a 1/500 dilution (Red).
Immunoprecipitation - Anti-p63 antibody (ab97865)
All lanes: Immunoprecipitation - Anti-p63 antibody (ab97865)
All lanes: A431 Whole cell extracts at 5 µg
SecondaryAll lanes: anti-Rabbit IgG
Predicted band size: 76 kDa
Western blot - Anti-p63 antibody (ab97865)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-p63 antibody (ab97865) at 1/1000 dilution
Lane 1: A431 Whole cell extracts at 30 µg
Lane 2: MCF-7 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 76 kDa
Western blot - Anti-p63 antibody (ab97865)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-p63 antibody (ab97865) at 1/500 dilution
All lanes: Mouse brain lysate/extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody (ab97865)
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using ab97865 at 1/100 dilution. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
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