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AB127690

Anti-p63RhoGEF antibody

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(7 Publications)

Rabbit Polyclonal p63RhoGEF antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF25 aa 200-450.

View Alternative Names

GEFT, ARHGEF25, Rho guanine nucleotide exchange factor 25, Guanine nucleotide exchange factor GEFT, Rac/Cdc42/Rho exchange factor GEFT, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT, p63RhoGEF

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63RhoGEF antibody (AB127690)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63RhoGEF antibody (AB127690)

ab127690, at a dilution of 1/250, staining p63RhoGEF in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

Western blot - Anti-p63RhoGEF antibody (AB127690)
  • WB

Unknown

Western blot - Anti-p63RhoGEF antibody (AB127690)

10% SDS PAGE

All lanes:

Western blot - Anti-p63RhoGEF antibody (ab127690) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARHGEF25 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

Q86VW2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

Keep as concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P63RhoGEF also known as RhoGEF GEF is a guanine nucleotide exchange factor that activates RhoA. This protein possesses a molecular mass of approximately 65 kDa. p63RhoGEF shows prominent expression in various tissues including the thymus lungs and brain. It activates RhoA by facilitating the exchange of GDP for GTP essential in RhoA signaling. As a member of the Dbl family of GEFs p63RhoGEF participates specifically with the GTPase activity of RhoA leading to various downstream effects.
Biological function summary

P63RhoGEF serves important roles in signaling pathways that regulate cytoskeletal dynamics and cell morphology. It does not typically form part of a complex but interacts directly with several proteins in cellular signaling. Through activation of RhoA p63RhoGEF influences processes such as cell migration division and adhesion. It plays a role in the reorganization of the actin cytoskeleton important for maintaining cellular shape and facilitating motile functions.

Pathways

P63RhoGEF functions within the RhoA signaling and GPCR pathways. It interacts with G-protein coupled receptors (GPCRs) to mediate signals that lead to the activation of RhoA. p63RhoGEF has connections to proteins like Gαq which directly influence its activity within these pathways. This interplay enables p63RhoGEF to translate extracellular signals into intracellular responses that affect cell structure and movement.

P63RhoGEF exhibits significant associations with cancer and cardiovascular diseases. Through its regulation of RhoA alterations in its activity can contribute to tumorigenesis and metastasis. Overactive RhoA signaling facilitated by p63RhoGEF can lead to increased cell migration and invasiveness frequently observed in cancer progression. Furthermore in the context of cardiovascular disease p63RhoGEF interacts with endothelial proteins influencing vascular tone and blood pressure implicating it in conditions like hypertension.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis (By similarity).
See full target information ARHGEF25
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:867 PubMed39616223

2024

GEFT inhibits the GSDM-mediated proptosis signalling pathway, promoting the progression and drug resistance of rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Tian Xia,Zhijuan Zhao,Jinyang Lin,Ling Zhong,Tian Tang,Degui Liao,Miaoling Lai,Jiamin Ceng,Lian Meng,Feng Li,Chunxia Liu

Frontiers in oncology 11:656608 PubMed34221974

2021

GEFT Inhibits Autophagy and Apoptosis in Rhabdomyosarcoma Activation of the Rac1/Cdc42-mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunsen Li,Zhenzhen Li,Lingxie Song,Lian Meng,Guixuan Xu,Haijun Zhang,Jianming Hu,Feng Li,Chunxia Liu

Frontiers in oncology 10:582669 PubMed33381451

2020

Long Non-Coding RNA LINC00355 Promotes the Development and Progression of Colorectal Cancer by Elevating Guanine Nucleotide Exchange Factor T Expression RNA Binding Protein lin-28 Homolog A.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Bing Zhang,Ge Gao,Yinping Zhang,Qingxin Xia

American journal of translational research 12:1136-1154 PubMed32269740

2020

MicroRNA-29 family inhibits rhabdomyosarcoma formation and progression by regulating GEFT function.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Liang Zhang,Yuweng Pang,Lingxie Song,Hao Shang,Zhenzhen Li,Qianqian Liu,Yangyang Zhang,Xiaomeng Wang,Qianru Li,Qiaochu Zhang,Chunxia Liu,Feng Li

Translational cancer research 9:346-357 PubMed35117188

2020

MIER3 suppresses the progression of non-small cell lung cancer by inhibiting Wnt/β-Catenin pathway and histone acetyltransferase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hongye Zhang,Ling Wang,Juan Bai,Wenyu Jiao,Mingxia Wang

EBioMedicine 50:122-134 PubMed31761617

2019

Epigenetically upregulated GEFT-derived invasion and metastasis of rhabdomyosarcoma via epithelial mesenchymal transition promoted by the Rac1/Cdc42-PAK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxia Liu,Liang Zhang,Wenwen Cui,Juan Du,Zhenzhen Li,Yuwen Pang,Qianqian Liu,Hao Shang,Lian Meng,Wanyu Li,Lingxie Song,Ping Wang,Yuwen Xie,Yuanyuan Wang,Yang Liu,Jianming Hu,Wenjie Zhang,Feng Li

Oncology letters 18:5577-5590 PubMed31620201

2019

GEFT protein expression in digestive tract malignant tumors and its clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Bing Zhang,Ge Gao,Yinping Zhang,Qingxin Xia
View all publications
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