Rabbit Polyclonal p63RhoGEF antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF25 aa 200-450.
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Publications
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- Frontiers in oncology 10:5826692020Long Non-Coding RNA LINC00355 Promotes the Development and Progression of Colorectal Cancer by Elevating Guanine Nucleotide Exchange Factor T Expression RNA Binding Protein lin-28 Homolog A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Wang et. al.PubMed 33381451
- American journal of translational research 12:1136-11542020MicroRNA-29 family inhibits rhabdomyosarcoma formation and progression by regulating GEFT function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Wang et. al.PubMed 32269740
- EBioMedicine 50:122-1342019Epigenetically upregulated GEFT-derived invasion and metastasis of rhabdomyosarcoma via epithelial mesenchymal transition promoted by the Rac1/Cdc42-PAK signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunxia Liu et. al.PubMed 31761617
- Oncology letters 18:5577-55902019GEFT protein expression in digestive tract malignant tumors and its clinical significance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Wang et. al.PubMed 31620201
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGEF25 aa 200-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VW2
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Target data
Function
May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis (By similarity).
Alternative names
GEFT, ARHGEF25, Rho guanine nucleotide exchange factor 25, Guanine nucleotide exchange factor GEFT, Rac/Cdc42/Rho exchange factor GEFT, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT, p63RhoGEF
Recommended products
Rabbit Polyclonal p63RhoGEF antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF25 aa 200-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGEF25 aa 200-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VW2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Keep as concentrated solution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P63RhoGEF also known as RhoGEF GEF is a guanine nucleotide exchange factor that activates RhoA. This protein possesses a molecular mass of approximately 65 kDa. p63RhoGEF shows prominent expression in various tissues including the thymus lungs and brain. It activates RhoA by facilitating the exchange of GDP for GTP essential in RhoA signaling. As a member of the Dbl family of GEFs p63RhoGEF participates specifically with the GTPase activity of RhoA leading to various downstream effects.
- Biological function summary
P63RhoGEF serves important roles in signaling pathways that regulate cytoskeletal dynamics and cell morphology. It does not typically form part of a complex but interacts directly with several proteins in cellular signaling. Through activation of RhoA p63RhoGEF influences processes such as cell migration division and adhesion. It plays a role in the reorganization of the actin cytoskeleton important for maintaining cellular shape and facilitating motile functions.
- Pathways
P63RhoGEF functions within the RhoA signaling and GPCR pathways. It interacts with G-protein coupled receptors (GPCRs) to mediate signals that lead to the activation of RhoA. p63RhoGEF has connections to proteins like Gαq which directly influence its activity within these pathways. This interplay enables p63RhoGEF to translate extracellular signals into intracellular responses that affect cell structure and movement.
- Associated diseases and disorders
P63RhoGEF exhibits significant associations with cancer and cardiovascular diseases. Through its regulation of RhoA alterations in its activity can contribute to tumorigenesis and metastasis. Overactive RhoA signaling facilitated by p63RhoGEF can lead to increased cell migration and invasiveness frequently observed in cancer progression. Furthermore in the context of cardiovascular disease p63RhoGEF interacts with endothelial proteins influencing vascular tone and blood pressure implicating it in conditions like hypertension.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-p63RhoGEF antibody (ab127690)
10% SDS PAGE
All lanes: Western blot - Anti-p63RhoGEF antibody (ab127690) at 1/1000 dilution
All lanes: IMR32 whole cell lysate at 30 µg
Predicted band size: 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63RhoGEF antibody (ab127690)
ab127690, at a dilution of 1/250, staining p63RhoGEF in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
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