AB131526

Anti-P70 S6 Kinase beta/SRK antibody

Name: Anti-P70 S6 Kinase beta/SRK antibody (ab131526) | Abcam
Brand: Abcam Limited
SKU: AB131526
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal P70 S6 Kinase beta/SRK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RPS6KB2 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

STK14B, RPS6KB2, Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, S6 kinase-related kinase, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, P70S6K2, p70-S6K 2, SRK, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p70 S6KB, p70-beta

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody (AB131526)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody (AB131526)

Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131526 at 1/100 dilution.

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Western blot - Anti-P70 S6 Kinase beta/SRK antibody (AB131526)

All lanes:

Western blot - Anti-P70 S6 Kinase beta/SRK antibody (ab131526) at 1/500 dilution

Lane 1:

293 cell lysate with blocking peptide

Lane 2:

293 cell lysate

Predicted band size: 53 kDa

Observed band size: 70 kDa,85 kDa

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CiteAb

Western blot - Anti-P70 S6 Kinase beta/SRK antibody (AB131526)

P70 S6 Kinase beta/SRK western blot using anti-P70 S6 Kinase beta/SRK antibody ab131526. Publication image and figure legend from Hao, L., Pang, K., et al., 2020, Aging (Albany NY), PubMed 32018225.

ab131526 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab131526 please see the product overview.

Knockdown of P3H4 arrested cell cycle in the G1 phase and thus inhibited proliferation, but had no effect on apoptosis. After siP3H4 was transfected into EJ and T24 cells, (A) the clonality was evaluated by clone formation assay, (B and C) cell cycle was detected by flow cytometry, (D) the protein expression of cell cycle-related proteins Cyclin D1, Nusap1 and p70 were measured by western blot, (E) apoptosis was examined by flow cytometry. *p<0.05.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human RPS6KB2 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UBS0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P70 S6 Kinase beta also known as SRK is a protein kinase involved in the regulation of cellular growth and metabolism. It has a molecular mass of approximately 70 kDa. This kinase is predominantly expressed in brain liver and muscle tissues. P70 is an important mediator in the process of protein translation acting mechanically by phosphorylating the ribosomal protein S6 which in turn enhances the translation of a subset of mRNAs related to cell growth processes.

Biological function summary

P70 S6 Kinase beta/SRK is a significant component in the cellular signaling complexes that manage cell size and energy regulation. It participates in the mechanistic target of rapamycin (mTOR) pathway. Through its enzymatic activity it helps cells respond to growth factors and nutrients. By motivating the translation of mRNAs p70 sustains ribosome biogenesis and cell growth.

Pathways

P70 operates within the mTOR signaling pathway known for its role in controlling cell growth and proliferation. It interacts with proteins like mTORC1 which is a known regulator of cellular metabolism and protein synthesis. These interactions allow p70 to integrate signals from other kinases like Akt influencing downstream responses including glucose metabolism.

P70 S6 Kinase beta/SRK links to conditions such as cancer and type 2 diabetes. Aberrant activation of P70 through the mTOR pathway contributes significantly to tumorigenesis due to its influence on cell growth and survival. Additionally in type 2 diabetes dysregulated signaling of P70 affects insulin sensitivity often in conjunction with related enzymes such as Akt and mTOR.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates specifically ribosomal protein S6 (PubMed : 29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed : 29750193).

See full target information RPS6KB2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:10944-10955 PubMed35477364

2022

GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haifeng Chen,Mingming Cheng,Pengcheng Gao,Xiangzhong Zhang,Ganggang Li,Liting Wang,Long Qin,Hongrui Li

OncoTargets and therapy 13:10973-10981 PubMed33149612

2020

Butorphanol Inhibits the Malignant Biological Behaviors of Ovarian Cancer Cells via Down-Regulating the Expression of TMEFF1.

Applications

Unspecified application

Species

Unspecified reactive species

Baosheng Wang,Yuwen Li,Yangyang Shen,Yong Xu,Chuanfeng Zhang

Aging 12:2156-2168 PubMed32018225

2020

Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Hao,Kun Pang,Hui Pang,Junjie Zhang,Zhiguo Zhang,Houguang He,Rongsheng Zhou,Zhenduo Shi,Conghui Han

American journal of translational research 10:4054-4064 PubMed30662650

2018

Atorvastatin regulates pericardial patch healing via the microRNA140-ADAM10-ephrinB2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Alan Dardik,Ren Guo,Weichang Zhang,Yuanyuan Xiang,Shuang Li,Dingxiao Liu,Chang Shu

Medical science monitor : international medical journal of experimental and clinical research 24:4982-4991 PubMed30018280

2018

Collapsin Response Mediator Protein-2 Ameliorates Sevoflurane-Mediated Neurocyte Injury by Targeting PI3K-mTOR-S6K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxuan He,Jianfang Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P70 S6 Kinase beta/SRK antibody

Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody (AB131526)

Western blot - Anti-P70 S6 Kinase beta/SRK antibody (AB131526)

Western blot - Anti-P70 S6 Kinase beta/SRK antibody (AB131526)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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