Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)

Intracellular Flow Cytometry analysis of2% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (green). Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)

Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 488) secondary at a 1/200 dilution (green) costained with Dapi (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)

Immunohistochemical analysis of paraffin-embedded Human pancreas labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/50 dilution counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)

All lanes:

Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

• WB

Lab

Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : RPS6KB2 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : Jurkat whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab184551 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab184551 was shown to specifically react with P70 S6 Kinase beta/SRK in wild-type HAP1 cells. No band was observed when RPS6KB2 knockout samples were used. Wild-type and RPS6KB2 knockout samples were subjected to SDS-PAGE. ab184551 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

Predicted band size: 53 kDa

false

• WB

Supplier Data

Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)

All lanes:

Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/10000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 53 kDa

false