Rabbit Recombinant Monoclonal P70 S6 Kinase beta/SRK antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 13 publications.
Images
![Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--western-blot-img141100.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)")
![Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--flow-cytometry-intracellular-img64314.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)")
![Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--immunocytochemistry-immunofluorescence-img93194.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106054.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)")
![Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--western-blot-img150015.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (AB184551)")
Publications
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- Oncology letters 23:702022CRNDE silencing promotes apoptosis and enhances cisplatin sensitivity of colorectal carcinoma cells by inhibiting the Akt/mTORC1-mediated Warburg effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Yang,Yanchun Wang,Chunhui Tao,Yunhai Li,Shan Cao,Xiqian YangPubMed 35069879
- Scientific reports 11:240052021Spontaneous formation of neutrophil extracellular traps is associated with autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Guo,Fei Gao,Xin Wang,Zhenzhen Pan,Qian Wang,Shiyao Xu,Shanshan Pan,Ling Li,Deyu Zhao,Jun QianPubMed 34907287
- Open medicine (Warsaw, Poland) 16:1364-13712021miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Juan Zhang,Fang Wang,Pei-Yan Qi,Wei-Yan Zhou,Bing WangPubMed 34589612
- Cell communication and signaling : CCS 18:1872020Pathogenic effects of inhibition of mTORC1/STAT3 axis facilitates Staphylococcus aureus-induced pyroptosis in human macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiyuan Yao et. al.PubMed 33256738
- International journal of molecular sciences 21:2020Proteomic Characterization of the Olfactory Molecular Imbalance in Dementia with Lewy Bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMercedes Lachén-Montes et. al.PubMed 32887355
- Cell death & disease 11:5962020Circ-GLI1 promotes metastasis in melanoma through interacting with p70S6K2 to activate Hedgehog/GLI1 and Wnt/β-catenin pathways and upregulate Cyr61.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Chen et. al.PubMed 32732916
- Frontiers in microbiology 11:11132020Triterpenoids Extracted From Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice Suppression Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYange Liu et. al.PubMed 32719658
- Experimental and therapeutic medicine 20:1145-11522020Prazosin inhibits the proliferation, migration and invasion, but promotes the apoptosis of U251 and U87 cells via the PI3K/AKT/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang et. al.PubMed 32765662
- Cell cycle (Georgetown, Tex.) 18:2239-22542019Silencing of EPCAM suppresses hepatic fibrosis and hepatic stellate cell proliferation in mice with alcoholic hepatitis via the PI3K/Akt/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi Zhang et. al.PubMed 31378124
- BioMed research international 2019:15282782019miR-451 Silencing Inhibited Doxorubicin Exposure-Induced Cardiotoxicity in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li et. al.PubMed 31355248
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Phosphorylates specifically ribosomal protein S6 (PubMed:29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed:29750193).
Alternative names
STK14B, RPS6KB2, Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, S6 kinase-related kinase, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, P70S6K2, p70-S6K 2, SRK, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p70 S6KB, p70-beta
Recommended products
Rabbit Recombinant Monoclonal P70 S6 Kinase beta/SRK antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14956
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P70 S6 Kinase beta also known as SRK is a protein kinase involved in the regulation of cellular growth and metabolism. It has a molecular mass of approximately 70 kDa. This kinase is predominantly expressed in brain liver and muscle tissues. P70 is an important mediator in the process of protein translation acting mechanically by phosphorylating the ribosomal protein S6 which in turn enhances the translation of a subset of mRNAs related to cell growth processes.
- Biological function summary
P70 S6 Kinase beta/SRK is a significant component in the cellular signaling complexes that manage cell size and energy regulation. It participates in the mechanistic target of rapamycin (mTOR) pathway. Through its enzymatic activity it helps cells respond to growth factors and nutrients. By motivating the translation of mRNAs p70 sustains ribosome biogenesis and cell growth.
- Pathways
P70 operates within the mTOR signaling pathway known for its role in controlling cell growth and proliferation. It interacts with proteins like mTORC1 which is a known regulator of cellular metabolism and protein synthesis. These interactions allow p70 to integrate signals from other kinases like Akt influencing downstream responses including glucose metabolism.
- Associated diseases and disorders
P70 S6 Kinase beta/SRK links to conditions such as cancer and type 2 diabetes. Aberrant activation of P70 through the mTOR pathway contributes significantly to tumorigenesis due to its influence on cell growth and survival. Additionally in type 2 diabetes dysregulated signaling of P70 affects insulin sensitivity often in conjunction with related enzymes such as Akt and mTOR.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--western-blot-img141100.jpg "Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)")
Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: RPS6KB2 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: Jurkat whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab184551 observed at 53 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab184551 was shown to specifically react with P70 S6 Kinase beta/SRK in wild-type HAP1 cells. No band was observed when RPS6KB2 knockout samples were used. Wild-type and RPS6KB2 knockout samples were subjected to SDS-PAGE. ab184551 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
Predicted band size: 53 kDa
![Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--flow-cytometry-intracellular-img64314.jpg "Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)")
Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
Intracellular Flow Cytometry analysis of2% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (green). Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution
![Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--immunocytochemistry-immunofluorescence-img93194.jpg "Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)")
Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 488) secondary at a 1/200 dilution (green) costained with Dapi (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106054.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
Immunohistochemical analysis of paraffin-embedded Human pancreas labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/50 dilution, counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--western-blot-img150015.jpg "Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)")
Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
All lanes: Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/50000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: K562 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
![Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--western-blot-img84677.jpg "Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)")
Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
All lanes: Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/10000 dilution
All lanes: Human fetal liver lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa
![Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551), expandable thumbnail](https://content.abcam.com/products/images/p70-s6-kinase-beta-srk-antibody-epr14956-n-terminal-ab184551--western-blot-img84677.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)")
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