AB247846

Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal P70 S6 Kinase beta/SRK phospho S371 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

STK14B, RPS6KB2, Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, S6 kinase-related kinase, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, P70S6K2, p70-S6K 2, SRK, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p70 S6KB, p70-beta

1 Images

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Western blot - Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] - BSA and Azide free (AB247846)

This data was developed using ab109393, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] (<a href='/en-us/products/primary-antibodies/p70-s6-kinase-beta-srk-phospho-s371-antibody-epr2132-ab109393'>ab109393</a>) at 1/1000 dilution

Lane 1:

293T cell lysate, untreated at 10 µg

Lane 2:

293T cell lysate, treated with Alkaline Phosphatase (AP) at 10 µg

Predicted band size: 53 kDa

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Unconjugated

Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2132

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects p70 S6 Kinase phosphorylated at serine 371.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab247846 is the carrier-free version of ab109393.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P70 S6 Kinase beta also known as SRK is a protein kinase involved in the regulation of cellular growth and metabolism. It has a molecular mass of approximately 70 kDa. This kinase is predominantly expressed in brain liver and muscle tissues. P70 is an important mediator in the process of protein translation acting mechanically by phosphorylating the ribosomal protein S6 which in turn enhances the translation of a subset of mRNAs related to cell growth processes.

Biological function summary

P70 S6 Kinase beta/SRK is a significant component in the cellular signaling complexes that manage cell size and energy regulation. It participates in the mechanistic target of rapamycin (mTOR) pathway. Through its enzymatic activity it helps cells respond to growth factors and nutrients. By motivating the translation of mRNAs p70 sustains ribosome biogenesis and cell growth.

Pathways

P70 operates within the mTOR signaling pathway known for its role in controlling cell growth and proliferation. It interacts with proteins like mTORC1 which is a known regulator of cellular metabolism and protein synthesis. These interactions allow p70 to integrate signals from other kinases like Akt influencing downstream responses including glucose metabolism.

P70 S6 Kinase beta/SRK links to conditions such as cancer and type 2 diabetes. Aberrant activation of P70 through the mTOR pathway contributes significantly to tumorigenesis due to its influence on cell growth and survival. Additionally in type 2 diabetes dysregulated signaling of P70 affects insulin sensitivity often in conjunction with related enzymes such as Akt and mTOR.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates specifically ribosomal protein S6 (PubMed : 29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed : 29750193).

See full target information RPS6KB2 phospho S371

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] - BSA and Azide free

Western blot - Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] - BSA and Azide free (AB247846)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise