Anti-P70 S6 Kinase (phospho T389) antibody (ab2571) is a rabbit polyclonal antibody that is used in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 50 publications
- Trusted since 2003
Images
Publications
Filter by
- Journal of cellular physiology 238:2827-28402023Mutual regulation between GDF11 and TET2 prevents senescence of mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaming Gao,Hao Wang,Junyan Shen,Xiaojing Liu,Xiaoqi Zhu,Ce Huang,Gongchen Li,Yao Sun,Zhongmin Liu,Yi Eve Sun,Hailiang LiuPubMed 37801347
- Frontiers in physiology 13:9497372022RagC GTPase regulates mTOR to promote chemoresistance in senescence-like HepG2 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Jiang,Zhenglin Ou,Qin Zhu,Hongyan ZaiPubMed 36267578
- Frontiers in nutrition 9:8573932022Comparison of the Effects of Inorganic or Amino Acid-Chelated Zinc on Mouse Myoblast Growth and Growth Performance and Carcass Traits in Growing-Finishing Pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingyu Zhang,Qiuping Guo,Yehui Duan,Xue Lin,Hengjia Ni,Chuanshe Zhou,Fengna LiPubMed 35464034
- Development (Cambridge, England) 148:2021Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Li,Jianan Tang,Xu Ji,Min-Min Hua,Miao Liu,Lu Chang,Yihua Gu,Changgen Shi,Wuhua Ni,Jing Liu,Hui-Juan Shi,Xuefeng Huang,Christopher O'Neill,Xingliang JinPubMed 34013332
- Theranostics 10:11754-117742020Cardiomyocyte-derived small extracellular vesicles can signal eNOS activation in cardiac microvascular endothelial cells to protect against Ischemia/Reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuihao Chen et. al.PubMed 33052245
- Journal of experimental & clinical cancer research 39:932020TFEB-NF-κB inflammatory signaling axis: a novel therapeutic pathway of Dihydrotanshinone I in doxorubicin-induced cardiotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Wang et. al.PubMed 32448281
- Biomedicines 8:2020PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoto Matsumoto et. al.PubMed 32316234
- International journal of molecular sciences 21:2020ColXV Aggravates Adipocyte Apoptosis by Facilitating Abnormal Extracellular Matrix Remodeling in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianyu Xia et. al.PubMed 32024006
- Neuroscience bulletin 36:289-2982019Propofol Attenuates α-Synuclein Aggregation and Neuronal Damage in a Mouse Model of Ischemic Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuzhu Wang,Dan Tian,Changwei Wei,Victoria Cui,Huan Wang,Yanbing Zhu,Anshi Wu,Yun YuePubMed 31520398
- The Journal of nutritional biochemistry 73:1082142019Formononetin alleviates hepatic steatosis by facilitating TFEB-mediated lysosome biogenesis and lipophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wang et. al.PubMed 31520816
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.877% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RPS6KB1 phospho T389. The exact immunogen used to generate this antibody is proprietary information. Database link P23443
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected |
| Rat | Expected | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes The optimal conditions should be determined individually. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes The optimal conditions should be determined individually. |
Species Rat | Dilution info 1/100 - 1/500 | Notes The optimal conditions should be determined individually. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-2 µg/mL | Notes This antibody detects bands at approximately 70 and 85 kDa. |
Species Human | Dilution info 1-2 µg/mL | Notes This antibody detects bands at approximately 70 and 85 kDa. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-2 µg/mL | Notes This antibody detects bands at approximately 70 and 85 kDa. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/200 | Notes - |
Species Rat | Dilution info 1/50 - 1/200 | Notes - |
Recommended products
Anti-P70 S6 Kinase (phospho T389) antibody (ab2571) is a rabbit polyclonal antibody that is used in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 50 publications
- Trusted since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.877% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RPS6KB1 phospho T389. The exact immunogen used to generate this antibody is proprietary information. Database link P23443
- Purification technique
- Affinity purification
- Specificity
Phospho-p70 S6 Kinase (Thr389/412) Ab detects endogenous levels of p70 S6 Kinase only when phosphorylated at Threonine 389/412
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-P70 S6 Kinase (phospho T389) antibody (ab2571) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-P70 S6 Kinase (phospho T389) (ab2571) was first used in a scientific publication in 2003 and has been cited over 50 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
P70 S6 Kinase (phospho T389) Western blot staining using rabbit Anti-P70 S6 Kinase (phospho T389) antibody
Western blot analysis of p70 S6 Kinase phosphorylation expression in Insulin treated
Jurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide.All lanes: Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Immunohistochemistry analysis of human liver cancer tissue sections staining Phospho-p70 S6 Kinase using ab2571 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
This image is courtesy of an anonymous customer reviewWestern blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
P70 S6 Kinase (phospho T389) Western blot staining using rabbit Anti-P70 S6 Kinase (phospho T389) antibody
Blocking Step: 5% Milk for 1 hour at 25°C.
All lanes: Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571) at 1/250 dilution
Lane 1: Normal Rat liver whole tissue lysate. at 20 µg
Lane 2: Rat liver whole tissue lysate 24 hours after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg
Lane 3: Rat liver whole tissue lysate 1 week after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Immunofluorescence analysis of Phospho-p70 S6 Kinase in 293 cells
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com