Rabbit Polyclonal antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human RPS6KB1 phospho T389.
Images
Publications
- Journal of cellular physiology 238:2827-28402023Mutual regulation between GDF11 and TET2 prevents senescence of mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaming Gao,Hao Wang,Junyan Shen,Xiaojing Liu,Xiaoqi Zhu,Ce Huang,Gongchen Li,Yao Sun,Zhongmin Liu,Yi Eve Sun,Hailiang LiuPubMed 37801347
- Frontiers in physiology 13:9497372022RagC GTPase regulates mTOR to promote chemoresistance in senescence-like HepG2 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Jiang,Zhenglin Ou,Qin Zhu,Hongyan ZaiPubMed 36267578
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.877% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human RPS6KB1 phospho T389. The exact immunogen used to generate this antibody is proprietary information. Database link P23443
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected |
| Rat | Expected | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes The optimal conditions should be determined individually. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes The optimal conditions should be determined individually. |
Species Rat | Dilution info 1/100 - 1/500 | Notes The optimal conditions should be determined individually. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-2 µg/mL | Notes This antibody detects bands at approximately 70 and 85 kDa. |
Species Human | Dilution info 1-2 µg/mL | Notes This antibody detects bands at approximately 70 and 85 kDa. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-2 µg/mL | Notes This antibody detects bands at approximately 70 and 85 kDa. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/200 | Notes - |
Species Rat | Dilution info 1/50 - 1/200 | Notes - |
Recommended products
Rabbit Polyclonal antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human RPS6KB1 phospho T389.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.877% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human RPS6KB1 phospho T389. The exact immunogen used to generate this antibody is proprietary information. Database link P23443
- Purification technique
Affinity purification
- Specificity
Phospho-p70 S6 Kinase (Thr389/412) Ab detects endogenous levels of p70 S6 Kinase only when phosphorylated at Threonine 389/412
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 3505 Phospho-p70 S6 Kinase Antibody. 3505-100 is the same size as the 100 μl size of ab2571.
p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5'oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Immunohistochemistry analysis of human liver cancer tissue sections staining Phospho-p70 S6 Kinase using ab2571 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
This image is courtesy of an anonymous customer reviewWestern blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Blocking Step: 5% Milk for 1 hour at 25°C.
All lanes: Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571) at 1/250 dilution
Lane 1: Normal Rat liver whole tissue lysate. at 20 µg
Lane 2: Rat liver whole tissue lysate 24 hours after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg
Lane 3: Rat liver whole tissue lysate 1 week after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 70 kDa
Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Western blot analysis of p70 S6 Kinase phosphorylation expression in Insulin treated
Jurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide.All lanes: Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Predicted band size: 59 kDa
Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Immunofluorescence analysis of Phospho-p70 S6 Kinase in 293 cells
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