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AB14430

Anti-p73 antibody

4

(4 Reviews)

|

(22 Publications)

Rabbit Polyclonal P73 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human TP73 aa 1-100.

View Alternative Names

P73, TP73, Tumor protein p73, p53-like transcription factor, p53-related protein

2 Images
Immunoprecipitation - Anti-p73 antibody (AB14430)
  • IP

Supplier Data

Immunoprecipitation - Anti-p73 antibody (AB14430)

Immunoprecipitation of p73 with ab14430 at 0.1 μg/mL.

Lane 1 and 2 : Different lots of ab14430 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 2 : IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-p73 antibody (ab14430)

Predicted band size: 69 kDa

false

Exposure time: 75s

Western blot - Anti-p73 antibody (AB14430)
  • WB

Supplier Data

Western blot - Anti-p73 antibody (AB14430)

All lanes:

Western blot - Anti-p73 antibody (ab14430) at 0.1 µg/mL

Lane 1:

HEK293 cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 50 µg

Lane 3:

HepG2 cell lysate at 50 µg

Lane 4:

RKO cell lysate at 50 µg

Predicted band size: 69 kDa

false

Exposure time: 75s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TP73 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O15350

Specificity

The antibody is not expected to react with deltaNp73.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "0.1 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was purified using an epitope specific to p73 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p73 also known in scientific literature as p-73 or simply p 73 is a member of the p53 family of transcription factors. Molecular weight of p73 is approximately 80 kDa. It is expressed widely in human tissues with high levels observed in the brain kidneys and heart. Structurally p73 is similar to p53 and shares many functional domains including a DNA-binding domain and an oligomerization domain.
Biological function summary

P73 plays a significant role in regulating cell cycle and apoptosis. It can activate transcription of several genes involved in these processes. p73 does not function alone; it can form complexes with other p53 family members and modulate cellular responses to stress. p73 also contributes to differentiation particularly in neuronal cells where it promotes survival and development.

Pathways

The activity of p73 is involved in important signaling routes such as the DNA damage response and the apoptosis pathway. It interacts with many other proteins including p53 to maintain genomic stability. The pathway connections illustrate that p73 acts as a critical node influencing cell fate and maintaining cellular health.

Research links p73 to conditions such as cancer and neurodevelopmental disorders. Abnormal expression or mutation of p73 is observed in various cancers often associated with altered p53 function. In neurodevelopmental disorders deficits in p73 expression can impair brain development and function. Understanding how p73 interacts with p53 in these contexts is important for developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression (By similarity). It is an essential factor for the positive regulation of lung ciliated cell differentiation (PubMed : 34077761).
See full target information TP73
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 13:1202585 PubMed37404750

2023

Lestaurtinib inhibits Citron kinase activity and medulloblastoma growth through induction of DNA damage, apoptosis and cytokinesis failure.

Applications

Unspecified application

Species

Unspecified reactive species

Gianmarco Pallavicini,Giorgia Iegiani,Roberta Parolisi,Alessia Ferraro,Francesca Garello,Valeria Bitonto,Enzo Terreno,Marta Gai,Ferdinando Di Cunto

iScience 25:103811 PubMed35198876

2022

p73-regulated FER1L4 lncRNA sponges the oncogenic potential of miR-1273g-3p and aids in the suppression of colorectal cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Apoorva Uboveja,Yatendra Kumar Satija,Fouzia Siraj,Daman Saluja

Scientific reports 11:20974 PubMed34697383

2021

Riluzole-induced apoptosis in osteosarcoma is mediated through Yes-associated protein upon phosphorylation by c-Abl Kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Marian Raghubir,Syeda M Azeem,Rifat Hasnat,Chowdhury N Rahman,Linda Wong,Salina Yan,Yu Qi Huang,Raquel Zhagui,Angelina Blyufer,Iffat Tariq,Cassey Tam,Sonam Lhamo,Lucas Cecilio,Yesmin Chowdhury,Shraddha ChandThakuri,Shahana S Mahajan

Cancers 13: PubMed33804489

2021

CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Iegiani,Marta Gai,Ferdinando Di Cunto,Gianmarco Pallavicini

Scientific reports 9:19367 PubMed31852961

2019

DNA Methylation Activates TP73 Expression in Hepatocellular Carcinoma and Gastrointestinal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixing Yao,Cristina Di Poto,Grace Mavodza,Everett Oliver,Habtom W Ressom,Zaki A Sherif

Scientific reports 9:950 PubMed30700826

2019

Synergistic activation of the NEU4 promoter by p73 and AP2 in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bi-He Cai,Po-Han Wu,Chi-Kan Chou,Hsiang-Chi Huang,Chia-Chun Chao,Hsiao-Yu Chung,Hsueh-Yi Lee,Jang-Yi Chen,Reiji Kannagi

Clinical cancer research : an official journal of the American Association for Cancer Research 25:2001-2017 PubMed30593514

2018

TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem-Like Cells in a Redox-Sensitive Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Tanveer Sharif,Cathleen Dai,Emma Martell,Mohammad Saleh Ghassemi-Rad,Mark Robert Hanes,Patrick J Murphy,Barry E Kennedy,Chitra Venugopal,Minomi Subapanditha,Carman A Giacomantonio,Paola Marcato,Sheila K Singh,Shashi Gujar

Cancer research 78:4599-4612 PubMed29921697

2018

Inactivation of Citron Kinase Inhibits Medulloblastoma Progression by Inducing Apoptosis and Cell Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Gianmarco Pallavicini,Francesco Sgrò,Francesca Garello,Mattia Falcone,Valeria Bitonto,Gaia E Berto,Federico T Bianchi,Marta Gai,Alessandra M A Chiotto,Miriam Filippi,Juan C Cutrin,Ugo Ala,Enzo Terreno,Emilia Turco,Ferdinando Di Cunto

Oncology letters 15:5175-5180 PubMed29552154

2018

Association between TAp73, p53 and VASH1 expression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wu,Zhihua Zhang,Fangxu Ma,Xiulong Zhang,Zhilin Zhang,Jianhua Tang,Ping Chen,Chunyan Zhou,Weiping Wang

Cell death & disease 8:e3034 PubMed28880267

2017

p73 is required for appropriate BMP-induced mesenchymal-to-epithelial transition during somatic cell reprogramming.

Applications

ChIP

Species

Unspecified reactive species

Marta Martin-Lopez,Laura Maeso-Alonso,Sandra Fuertes-Alvarez,Diego Balboa,Virginia Rodríguez-Cortez,Jere Weltner,Inmaculada Diez-Prieto,Andrew Davis,Yaning Wu,Timo Otonkoski,Elsa R Flores,Pablo Menéndez,Margarita M Marques,Maria C Marin
View all publications
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