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AB218625

Anti-p73 (phospho Y99) antibody [EPR21751]

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Rabbit Recombinant Monoclonal P73 phospho Y99 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples.

View Alternative Names

P73, TP73, Tumor protein p73, p53-like transcription factor, p53-related protein

3 Images
Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (AB218625)
  • IP

Supplier Data

Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (AB218625)

p73 (phospho Y99) was immunoprecipitated from 0.35 mg of HT-1376 (human urinary bladder carcinoma cell line) treated with 1mM Pervanadate for 30 min whole cell lysate with ab218625 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218625 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

Lane 1 : HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate 10 μg (input).

Lane 2 : ab218625 IP in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab218625 in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)

Predicted band size: 69 kDa

false

Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] (AB218625)
  • WB

Supplier Data

Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] (AB218625)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID : 11466610).

All lanes:

Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625) at 1/1000 dilution

Lane 1:

Untreated HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 80 kDa

true

Exposure time: 15s

Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] (AB218625)
  • Dot

Supplier Data

Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] (AB218625)

Dot blot analysis of p73 (phospho Y99) with ab218625 at 1/1000 dilution.

Lane 1 : p73 (phospho Y99) peptide.

Lane 2 : p73 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21751

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p73 also known in scientific literature as p-73 or simply p 73 is a member of the p53 family of transcription factors. Molecular weight of p73 is approximately 80 kDa. It is expressed widely in human tissues with high levels observed in the brain kidneys and heart. Structurally p73 is similar to p53 and shares many functional domains including a DNA-binding domain and an oligomerization domain.
Biological function summary

P73 plays a significant role in regulating cell cycle and apoptosis. It can activate transcription of several genes involved in these processes. p73 does not function alone; it can form complexes with other p53 family members and modulate cellular responses to stress. p73 also contributes to differentiation particularly in neuronal cells where it promotes survival and development.

Pathways

The activity of p73 is involved in important signaling routes such as the DNA damage response and the apoptosis pathway. It interacts with many other proteins including p53 to maintain genomic stability. The pathway connections illustrate that p73 acts as a critical node influencing cell fate and maintaining cellular health.

Research links p73 to conditions such as cancer and neurodevelopmental disorders. Abnormal expression or mutation of p73 is observed in various cancers often associated with altered p53 function. In neurodevelopmental disorders deficits in p73 expression can impair brain development and function. Understanding how p73 interacts with p53 in these contexts is important for developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression (By similarity). It is an essential factor for the positive regulation of lung ciliated cell differentiation (PubMed : 34077761).
See full target information TP73 phospho Y99
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Product promise

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