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Mouse Monoclonal p75 NGF Receptor antibody. Suitable for IP, ICC/IF, Flow Cyt, WB and reacts with Rat, Human, Mouse, Transfected cell line - Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NGFR aa 1-250.

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Images

Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail
  • Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail
  • Western blot - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7 - 7.4
Constituents: PBS, 3% Trehalose

Form

Lyophilized

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human NGFR aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P08138

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFFlow CytWB
Human
Expected
Expected
Tested
Tested
Mouse
Expected
Expected
Expected
Predicted
Rat
Tested
Tested
Tested
Tested
Transfected cell line - Mouse
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Rat

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

-

Notes

-

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1-5 µg/mL

Notes

Light fixation only or unfixed works best. Epitope is sensitive to fixation.

Expected
Expected

Species

Human

Dilution info

-

Notes

-

Species

Mouse

Dilution info

1-5 µg/mL

Notes

Light fixation only or unfixed works best. Epitope is sensitive to fixation.

Not recommended
Not recommended

Species

Transfected cell line - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5-20 µg/mL

Notes

-

Species

Rat

Dilution info

5-20 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

5-20 µg/mL

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Mouse

Dilution info

0.5-2 µg/mL

Notes

Non-reducing conditions only - no DTT or beta-mercapoethanol.

Species

Rat

Dilution info

0.5-2 µg/mL

Notes

Non-reducing conditions only - no DTT or beta-mercapoethanol.

Species

Human

Dilution info

0.5-2 µg/mL

Notes

Non-reducing conditions only - no DTT or beta-mercapoethanol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed:24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed:26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed:23785138).

Alternative names

Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, p75 ICD, NGF receptor, TNFRSF16, NGFR

Recommended products

Mouse Monoclonal p75 NGF Receptor antibody. Suitable for IP, ICC/IF, Flow Cyt, WB and reacts with Rat, Human, Mouse, Transfected cell line - Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NGFR aa 1-250.

Alternative names

Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, p75 ICD, NGF receptor, TNFRSF16, NGFR

Key facts

Isotype

IgG2a

Form

Lyophilized

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human NGFR aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P08138
Clone number

8J2

Purification technique

Affinity purification Protein A

Reconstitution

Reconstitute with 100 microlitre sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

Associated diseases and disorders

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail

    Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (ab245134)

    Flow Cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) (Left panel) and C6 (rat glial tumor cell line) (Right panel) staining p75 NGF Receptor using ab245134 (red lines) and a different antibody (solid black lines) at 2 μg per 106 cells, 60 mins on ice. Negative control is the dashed line, Non specific Control IgG.

    Blocking: 200 μg/mL sheep IgG, 30 minutes on ice.

    Secondary antibody: Goat anti-mouse-PE used at a 1/100 dilution for 20 mins in the dark on ice.

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (ab245134)

    Non-fixed and 4% formaldehyde-fixed C6 (rat glial tumor cell line) cells labeling p75 NGF Receptor using ab245134 at 2 μg/ml for 1 hour (green) in ICC/IF.

    Blocking: 10% normal horse serum, 30 mins.

    Secondary antibody: Donkey anti-mouse-CF488A at 2 μg/mL for 1 hour.

    Nuclei stained with Hoechst (blue).

  • Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail

    Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134)

    Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134) (10 μg antibody per 200 μg total protein):

    Lane 1: p75 NGF Receptor IP in C6 (rat glial tumor cell line) whole cell lysate.

    Lane 2: Control Protein G agarose (10 μL).

    Precipitated p75 NGF Receptor was eluted off the beads and antibody by heating and addition of SDS-PAGE sample buffer.

    WB method: ab245134 (1 μg/mL) and secondary anti-sheep-HRP (1/6000) antibody.

    Detection: Chemiluminiscence.

    All lanes: Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134)

    Predicted band size: 45 kDa

  • Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail

    Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134)

    SDS-PAGE: 4-12%, non-reducing conditions.

    Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 μm).

    Detection: Chemiluminiscence.

    All lanes: Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134) at 1 µg/mL

    Lane 1: Mouse p75 NGF Receptor-transfected cells at 50 µg

    Lane 2: Non-transfected control cells at 50 µg

    Lane 3: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 50 µg

    Lane 4: C6 (rat glial tumor cell line) cell lysate at 50 µg

    Secondary

    All lanes: anti-mouse-HRP, 1 hour at RT at 1/6000 dilution

    Predicted band size: 45 kDa

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Product protocols

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