AB245134

Anti-p75 NGF Receptor antibody [8J2]

Name: Anti-p75 NGF Receptor antibody [8J2] (ab245134) | Abcam
Brand: Abcam Limited
SKU: AB245134
Price: 415 USD
Availability: InStock

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(10 Publications)

Mouse Monoclonal p75 NGF Receptor antibody. Suitable for IP, ICC/IF, Flow Cyt, WB and reacts with Rat, Human, Mouse, Transfected cell line - Mouse samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NGFR aa 1-250.

View Alternative Names

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

4 Images

Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

Flow Cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) (Left panel) and C6 (rat glial tumor cell line) (Right panel) staining p75 NGF Receptor using ab245134 (red lines) and a different antibody (solid black lines) at 2 μg per 10⁶ cells, 60 mins on ice. Negative control is the dashed line, Non specific Control IgG.

Blocking : 200 μg/mL sheep IgG, 30 minutes on ice.

Secondary antibody : Goat anti-mouse-PE used at a 1/100 dilution for 20 mins in the dark on ice.

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

Non-fixed and 4% formaldehyde-fixed C6 (rat glial tumor cell line) cells labeling p75 NGF Receptor using ab245134 at 2 μg/ml for 1 hour (green) in ICC/IF.

Blocking : 10% normal horse serum, 30 mins.

Secondary antibody : Donkey anti-mouse-CF488A at 2 μg/mL for 1 hour.

Nuclei stained with Hoechst (blue).

Supplier Data

Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134) (10 μg antibody per 200 μg total protein) :

Lane 1 : p75 NGF Receptor IP in C6 (rat glial tumor cell line) whole cell lysate.

Lane 2 : Control Protein G agarose (10 μL).

Precipitated p75 NGF Receptor was eluted off the beads and antibody by heating and addition of SDS-PAGE sample buffer.

WB method : ab245134 (1 μg/mL) and secondary anti-sheep-HRP (1/6000) antibody.

Detection : Chemiluminiscence.

All lanes:

Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134)

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

SDS-PAGE : 4-12%, non-reducing conditions.

Transfer : Tris-Glycine buffer; Membrane : nitrocellulose (0.45 μm).

Detection : Chemiluminiscence.

All lanes:

Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134) at 1 µg/mL

Lane 1:

Mouse p75 NGF Receptor-transfected cells at 50 µg

Lane 2:

Non-transfected control cells at 50 µg

Lane 3:

SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 50 µg

Lane 4:

C6 (rat glial tumor cell line) cell lysate at 50 µg

Secondary

All lanes:

anti-mouse-HRP, 1 hour at RT at 1/6000 dilution

Predicted band size: 45 kDa

false

519 FITC

FITC Anti-p75 NGF Receptor antibody [8J2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8J2

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human NGFR aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P08138

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5-20 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "Non-reducing conditions only - no DTT or beta-mercapoethanol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "Light fixation only or unfixed works best. Epitope is sensitive to fixation.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "5-20 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "Light fixation only or unfixed works best. Epitope is sensitive to fixation.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5-20 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "Non-reducing conditions only - no DTT or beta-mercapoethanol." }, "Transfected cell line - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "Non-reducing conditions only - no DTT or beta-mercapoethanol." } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 100µL

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7 - 7.4 Constituents: PBS, 3% Trehalose

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138). Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed : 22566094).

See full target information NGFR

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8160 PubMed40890143

2025

Capillary constrictions prime cancer cell tumorigenicity through PIEZO1.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Silvani,Chantal Kopecky,Sara Romanazzo,Vanina Rodríguez,Ayan Das,Elvis Pandzic,John G Lock,Christine L Chaffer,Kate Poole,Kristopher A Kilian

Nature communications 16:4598 PubMed40382324

2025

Auricular malformations are driven by copy number variations in a hierarchical enhancer cluster and a dominant enhancer recapitulates human pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Xu,Qi Chen,Qingpei Huang,Timothy C Cox,Hao Zhu,Jintian Hu,Xi Han,Ziqiu Meng,Bingqing Wang,Zhiying Liao,Wenxin Xu,Baichuan Xiao,Ruirui Lang,Jiqiang Liu,Jian Huang,Xiaokai Tang,Jinmo Wang,Qiang Li,Ting Liu,Qingguo Zhang,Stylianos E Antonarakis,Jiao Zhang,Xiaoying Fan,Huisheng Liu,Yong-Biao Zhang

Nature protocols 20:810-842 PubMed39501108

2024

Life-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Bibek R Thapa,Jake A Le Suer,Andrew Tilston-Lünel,Michael J Herriges,Feiya Wang,Pushpinder S Bawa,Xaralabos Varelas,Finn J Hawkins,Darrell N Kotton

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 18:476-494 PubMed37658943

2023

Curcumin-activated Olfactory Ensheathing Cells Improve Functional Recovery After Spinal Cord Injury by Modulating Microglia Polarization Through APOE/TREM2/NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Jiang,Zhe Chen,Xiaohui Wang,Yongyuan Zhang,Xinyu Guo,Hong Fan,Dageng Huang,Yuqing He,Xiangwen Tang,Yixiang Ai,Youjun Liu,Hao Yang,Dingjun Hao

Cell stem cell 30:1199-1216.e7 PubMed37625411

2023

Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Bibek R Thapa,Jake A Le Suer,Andrew Tilston-Lünel,Michael J Herriges,Andrew Berical,Mary Lou Beermann,Feiya Wang,Pushpinder S Bawa,Anat Kohn,Alexandra B Ysasi,Hirofumi Kiyokawa,Taylor M Matte,Scott H Randell,Xaralabos Varelas,Finn J Hawkins,Darrell N Kotton

CNS neuroscience & therapeutics 29:3239-3258 PubMed37157936

2023

LIPUS-SCs-Exo promotes peripheral nerve regeneration in cavernous nerve crush injury-induced ED rats via PI3K/Akt/FoxO signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Ye,Zitaiyu Li,Yinghao Yin,Jun Zhou,Dongjie Li,Yu Gan,Dongyi Peng,Ming Xiao,Liangyu Zhao,Yingbo Dai,Yuxin Tang

Andrology 11:1188-1202 PubMed36762774

2023

Low-intensity pulsed ultrasound ameliorates erectile dysfunction induced by bilateral cavernous nerve injury through enhancing Schwann cell-mediated cavernous nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zitaiyu Li,Kun Ye,Yinghao Yin,Jun Zhou,Dongjie Li,Yu Gan,Dongyi Peng,Liangyu Zhao,Ming Xiao,Yihong Zhou,Yingbo Dai,Yuxin Tang

International journal of molecular sciences 23: PubMed36142746

2022

Engineering Nanofiber Scaffolds with Biomimetic Cues for Differentiation of Skin-Derived Neural Crest-like Stem Cells to Schwann Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ashis Kumar Podder,Mohamed Alaa Mohamed,Georgios Tseropoulos,Bita Nasiri,Stelios T Andreadis

Frontiers in physiology 13:981311 PubMed36213234

2022

A potential role of in the regulation of circadian rhythm and incremental growth lines during tooth development.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Yuan,Bo Xie,Xia Yu,Cheng Lin,Meng Li,Yixin Zhang,Xuqiang Zou,Mingjie Lu,Manzhu Zhao,Xiujie Wen

PLoS genetics 15:e1008184 PubMed31125351

2019

Drosophila ADCK1 is critical for maintaining mitochondrial structures and functions in the muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Woongchang Yoon,Sun-Hong Hwang,Sang-Hee Lee,Jongkyeong Chung

View all publications

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Product promise

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Anti-p75 NGF Receptor antibody [8J2]

Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

Western blot - Anti-p75 NGF Receptor antibody [8J2] (AB245134)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise