Mouse Monoclonal p75 NGF Receptor antibody. Suitable for IP, ICC/IF, Flow Cyt, WB and reacts with Rat, Human, Mouse, Transfected cell line - Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NGFR aa 1-250.
Images
![Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--flow-cytometry-img98596.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (AB245134)")
![Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--immunocytochemistry-immunofluorescence-img42274.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (AB245134)")
![Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--immunoprecipitation-img1176.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (AB245134)")
![Western blot - Anti-p75 NGF Receptor antibody [8J2] (AB245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--western-blot-img930.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p75 NGF Receptor antibody [8J2] (AB245134)")
Publications
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- Andrology 11:1188-12022023Low-intensity pulsed ultrasound ameliorates erectile dysfunction induced by bilateral cavernous nerve injury through enhancing Schwann cell-mediated cavernous nerve regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZitaiyu Li,Kun Ye,Yinghao Yin,Jun Zhou,Dongjie Li,Yu Gan,Dongyi Peng,Liangyu Zhao,Ming Xiao,Yihong Zhou,Yingbo Dai,Yuxin TangPubMed 36762774
- Frontiers in physiology 13:9813112022A potential role of in the regulation of circadian rhythm and incremental growth lines during tooth development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Yuan,Bo Xie,Xia Yu,Cheng Lin,Meng Li,Yixin Zhang,Xuqiang Zou,Mingjie Lu,Manzhu Zhao,Xiujie WenPubMed 36213234
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7 - 7.4
Constituents: PBS, 3% Trehalose- Form
- Lyophilized
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human NGFR aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P08138
Reactivity data
Select an application| IP | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Expected | Expected | Tested | Tested |
| Mouse | Expected | Expected | Expected | Predicted |
| Rat | Tested | Tested | Tested | Tested |
| Transfected cell line - Mouse | Not recommended | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-5 µg/mL | Notes Light fixation only or unfixed works best. Epitope is sensitive to fixation. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Mouse | Dilution info 1-5 µg/mL | Notes Light fixation only or unfixed works best. Epitope is sensitive to fixation. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
Species Rat | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5-20 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info 0.5-2 µg/mL | Notes Non-reducing conditions only - no DTT or beta-mercapoethanol. |
Species Rat | Dilution info 0.5-2 µg/mL | Notes Non-reducing conditions only - no DTT or beta-mercapoethanol. |
Species Human | Dilution info 0.5-2 µg/mL | Notes Non-reducing conditions only - no DTT or beta-mercapoethanol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed:24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed:26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed:23785138).
Alternative names
CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor
Recommended products
Mouse Monoclonal p75 NGF Receptor antibody. Suitable for IP, ICC/IF, Flow Cyt, WB and reacts with Rat, Human, Mouse, Transfected cell line - Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NGFR aa 1-250.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7 - 7.4
Constituents: PBS, 3% Trehalose- Form
- Lyophilized
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human NGFR aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P08138
- Clone number
- 8J2
- Purification technique
- Affinity purification Protein A
- Reconstitution
- reconstitute with water at 100µL
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.
- Biological function summary
The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.
- Pathways
The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.
- Associated diseases and disorders
P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--flow-cytometry-img98596.jpg "Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (ab245134)")
Flow Cytometry - Anti-p75 NGF Receptor antibody [8J2] (ab245134)
Flow Cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) (Left panel) and C6 (rat glial tumor cell line) (Right panel) staining p75 NGF Receptor using ab245134 (red lines) and a different antibody (solid black lines) at 2 μg per 106 cells, 60 mins on ice. Negative control is the dashed line, Non specific Control IgG.
Blocking: 200 μg/mL sheep IgG, 30 minutes on ice.
Secondary antibody: Goat anti-mouse-PE used at a 1/100 dilution for 20 mins in the dark on ice.
![Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--immunocytochemistry-immunofluorescence-img42274.jpg "Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (ab245134)")
Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [8J2] (ab245134)
Non-fixed and 4% formaldehyde-fixed C6 (rat glial tumor cell line) cells labeling p75 NGF Receptor using ab245134 at 2 μg/ml for 1 hour (green) in ICC/IF.
Blocking: 10% normal horse serum, 30 mins.
Secondary antibody: Donkey anti-mouse-CF488A at 2 μg/mL for 1 hour.
Nuclei stained with Hoechst (blue).
![Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--immunoprecipitation-img1176.jpg "Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134)")
Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134)
Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134) (10 μg antibody per 200 μg total protein):
Lane 1: p75 NGF Receptor IP in C6 (rat glial tumor cell line) whole cell lysate.
Lane 2: Control Protein G agarose (10 μL).
Precipitated p75 NGF Receptor was eluted off the beads and antibody by heating and addition of SDS-PAGE sample buffer.
WB method: ab245134 (1 μg/mL) and secondary anti-sheep-HRP (1/6000) antibody.
Detection: Chemiluminiscence.
All lanes: Immunoprecipitation - Anti-p75 NGF Receptor antibody [8J2] (ab245134)
Predicted band size: 45 kDa
![Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134), expandable thumbnail](https://content.abcam.com/products/images/p75-ngf-receptor-antibody-8j2-ab245134--western-blot-img930.jpg "Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134)")
Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134)
SDS-PAGE: 4-12%, non-reducing conditions.
Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 μm).
Detection: Chemiluminiscence.
All lanes: Western blot - Anti-p75 NGF Receptor antibody [8J2] (ab245134) at 1 µg/mL
Lane 1: Mouse p75 NGF Receptor-transfected cells at 50 µg
Lane 2: Non-transfected control cells at 50 µg
Lane 3: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 50 µg
Lane 4: C6 (rat glial tumor cell line) cell lysate at 50 µg
SecondaryAll lanes: anti-mouse-HRP, 1 hour at RT at 1/6000 dilution
Performed under non-reducing conditions.
Predicted band size: 45 kDa
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