AB216495

Anti-p75 NGF Receptor antibody

Name: Anti-p75 NGF Receptor antibody (ab216495) | Abcam
Brand: Abcam Limited
SKU: AB216495
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal p75 NGF Receptor antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Ngfr aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB216495)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB216495)

Immunofluorescent analysis of HepG2 cells labeling p75 NGF Receptor with ab216495 at 1/500 dilution in PBS (incubated for two hours at 37°C), followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue labeling p75 NGF Receptor with ab216495 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, PE conjugated at 1/200 dilution for 40 minutes at 37°C.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cytometry - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cyt

Unknown

Flow Cytometry - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cytometry of Mouse Spleen with ab217628 at 1/100

Supplier Data

Western blot - Anti-p75 NGF Receptor antibody (AB216495)

All lanes:

Western blot - Anti-p75 NGF Receptor antibody (ab216495) at 1/300 dilution

All lanes:

Mouse brain lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Rat Ngfr aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07174

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "(or 1/50 - 1/200 if using a fluorescent secondary antibody).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138). Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed : 22566094).

See full target information NGFR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:330 PubMed40886189

2025

Melatonin attenuates intervertebral disc degeneration by restoring mitochondrial homeostasis through PGC-1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Lan,Wenhan Yang,Bin Yan,Weizhuang Guo,Yuantao Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p75 NGF Receptor antibody

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB216495)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cytometry - Anti-p75 NGF Receptor antibody (AB216495)

Western blot - Anti-p75 NGF Receptor antibody (AB216495)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise