AB227509

Anti-p75 NGF Receptor antibody

Name: Anti-p75 NGF Receptor antibody (ab227509) | Abcam
Brand: Abcam Limited
SKU: AB227509
Price: 525 USD
Availability: InStock

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(4 Publications )

Rabbit Polyclonal p75 NGF Receptor antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NGFR.

View Alternative Names

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB227509)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB227509)

Paraffin-embedded rat brain tissue stained for p75 NGF Receptor with ab227509 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody (AB227509)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody (AB227509)

Frozen section of rat E13.5 brain tissue stained for p75 NGF Receptor (green) using ab227509 at 1/250 dilution in immunohistochemical analysis.

Counterstain : Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red). Nuclear counterstain : Fluroshield with DAPI (blue).

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB227509)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB227509)

Immunofluorescence analysis of DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling p75 NGF Receptor / CD271 protein using ab227509 at a 1/500 dilution (Green). Tau, stained by Tau antibody used at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

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Western blot - Anti-p75 NGF Receptor antibody (AB227509)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution

All lanes:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-p75 NGF Receptor antibody (AB227509)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NGFR. The exact immunogen used to generate this antibody is proprietary information.

P08138

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138). Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed : 22566094).

See full target information NGFR

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113041 PubMed40822354

2025

Cholinotrophic basal forebrain connectome dysfunction in Down syndrome with and without dementia.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Moreno-Rodriguez,Sylvia E Perez,Muhammad Nadeem,Elizabeth Head,Elliott J Mufson

The Korean journal of pain 35:160-172 PubMed35354679

2022

Insulin enhances neurite extension and myelination of diabetic neuropathy neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Vuong M Pham,Nitish Thakor

Nature communications 10:4579 PubMed31594926

2019

HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liang,Jie Li,Cheng Lu,Duoli Xie,Jin Liu,Chuanxin Zhong,Xiaohao Wu,Rongchen Dai,Huarui Zhang,Daogang Guan,Baosheng Guo,Bing He,Fangfei Li,Xiaojuan He,Wandong Zhang,Bao-Ting Zhang,Ge Zhang,Aiping Lu

Neoplasma 66:427-436 PubMed30784290

2019

MicroRNA-363-3p/p21(Cip1/Waf1) axis is regulated by HIF-2α in mediating stemness of melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

T Hao,C X Li,X Y Ding,X J Xing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-p75 NGF Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB227509)

Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody (AB227509)

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB227509)

Western blot - Anti-p75 NGF Receptor antibody (AB227509)

Western blot - Anti-p75 NGF Receptor antibody (AB227509)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise