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Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 is a rabbit monoclonal antibody that is used in p75 NGF Receptor western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1039Y has been tried and trusted by researchers since 2007 and is cited in >100 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your p75 NGF Receptor staining, use in p75 NGF Receptor western blotting, IHC, immunofluorescence and flow cytometry


Images

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] (AB52987), expandable thumbnail
  • Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] (AB52987), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (AB52987), expandable thumbnail
  • Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (AB52987), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (AB52987), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Expected
Expected
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Tested
Tested
Tested
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/60

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

1 product for Alternative Version

Target data

Function

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed:24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed:26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed:23785138).

Alternative names

Recommended products

Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 is a rabbit monoclonal antibody that is used in p75 NGF Receptor western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1039Y has been tried and trusted by researchers since 2007 and is cited in >100 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your p75 NGF Receptor staining, use in p75 NGF Receptor western blotting, IHC, immunofluorescence and flow cytometry

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1039Y

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Dissociation constant

3.25 x 10-10 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

Associated diseases and disorders

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail
    Image from Abe SI. et al. PLoS One. 2017 Nov 30;12(11):e0188705. doi: 10.1371/journal.pone.0188705.

    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    The differentiation capacity of purified CD34+ cells cultured for 3 days in the presence or absence of ALK5i was evaluated by performing immunofluorescence analysis assessing whether CD34+ cells had changed to cells expressing p75 and/or α-SMA. Expressions of CD34, p75 and α-SMA were assessed by immunofluorescence on day 0 (F-H), day 3 in SP+f medium (I-K), or day 3 in the same medium as (I-K) but with ALK5i (L-N).

    Cultured re-aggregates were fixed in 4% PFA and embedded in paraffin. Sections (5 ∝m) were boiled in 0.01 M citrate (pH 6.0) with 0.1% Tween 20 for 10 min, washed three times in 0.1% Tween-20/PBS, transferred to blocking solution containing 5% BSA and 5% horse serum (Sigma) or goat serum (Invitrogen) in 0.1% Triton X-100/PBS for 1 hr, and incubated with primary antibody (p75 at 1/100 dilution) at 4°C overnight. After washing, the secondary antibody was added, and the sections were incubated for 2 hrs at room temperature. Microscopic images were obtained using a CCD camera (DP72, Olympus, Tokyo) mounted on a fluorescence microscope (BX60, or BX61VS-ASW, Olympus). Cultured cells on coverglasses were fixed in 4% PFA. Antigen retrieval was done by incubation with 100% methanol (-20°C) 10 min, and 0.3% Triton X-100 for 10 min.

  • Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Lane 1 (input): PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10μg
    Lane 2 (+): PC-12 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52987 in PC-12 whole cell lysate

    ab52987 immunoprecipitating p75 NGF receptor in PC-12 whole cell lysates. For western blotting, primary antibody used was ab52987 at 1.6 μg/ml. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution. Capture antibody was used at 1:40 dilution (2μg in 0.35mg lysates).

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure: 10 seconds

    All lanes: Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Predicted band size: 45 kDa

    Observed band size: 75 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Purified ab52987 staining p75 NGF receptor in paraffin embedded Human tonsil tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 3.3μg/ml. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on germinal centre of human tonsil.

  • Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Blocking and diluting buffer: 5% NFDM/TBST

    ab52987 fails to detect band of interest in Capan-1 (positive, PMID: 14613990) and brain lysates (positive, PMID: 21413144, 21541365), indicating its low affinity in some p75 NGF Receptor positive materials.

    All lanes: Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987) at 1/1000 dilution

    Lane 1: Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: Mouse uterus tissue lysates at 20 µg

    Lane 3: Mouse brain tissue lysates at 20 µg

    Lane 4: Rat uterus tissue lysates at 20 µg

    Lane 5: Rat brain tissue lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 45 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail
    Image from Li Y et al. Reprod Biol Endocrinol. 2011 Mar 8;9:30. Fig 2.; doi:10.1186/1477-7827-9-30; 8 March 2011 Reproductive Biology and Endocrinology 2011 9:30.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Immunohistochemical analysis of murine uterus tissue with adenomyosis, staining p75 NGF Receptor with ab52987.


    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6). Tissue was blocked with goat serum for 15 minutes before incubating with primary antibody (1/100) overnight at 4°C. A biotinylated goat anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.

  • Flow Cytometry (Intracellular) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Intracellular Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with purified ab52987 at 1/80 dilution (1ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Blocking and diluting buffer: 5% NFDM/TBST

    ab52987 fails to detect band of interest in hippocampus and cortex lysates (positive, PMID: 25180603, 28507518, 18930453, 20937383, 21059364), indicating its low affinity in some p75 NGF Receptor positive materials.

    Exposure: Lane 1-3: 8 seconds
    Lane 4-6: 180 seconds
    Lane 7-9: 30 seconds


    All lanes: Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987) at 1/1000 dilution

    Lane 1: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: Human hippocampus tissue lysates at 20 µg

    Lane 3: Human brain cortex tissue lysates at 20 µg

    Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

    Lane 5: Mouse hippocampus tissue lysates at 20 µg

    Lane 6: Mouse cerebral cortex lysates at 20 µg

    Lane 7: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

    Lane 8: Rat hippocampus tissue lysates at 20 µg

    Lane 9: Rat brain cortex tissue lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Purified ab52987 staining p75 NGF receptor in PC-12 (rat adrenal gland pheochromocytoma) by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 3.9 μg/ml. An AlexaFluor®488 Goat anti-Rabbit was used as the secondary antibody at 2 μg/ml. DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic and Membranous staining in PC-12 cells.

  • Flow Cytometry (Intracellular) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Intracellular Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with unpurified ab52987 at 1/60 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    All lanes: Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987) at 1/50000 dilution

    All lanes: PC12 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP labelled at 1/2000 dilution

    Predicted band size: 45 kDa

    Observed band size: 75 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    ICC/IF image of ab52987 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52987, 1 μg/mL) overnight at 4oC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluo® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.4 μM.

  • OI-RD Scanning - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    OI-RD Scanning - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

    Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling p75 NGF Receptor with ab52987 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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