Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)

Rabbit Recombinant Monoclonal p75 NGF Receptor antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 10 publications.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (AB221212), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Expected	Tested	Expected	Expected	Tested
Mouse	Expected	Tested	Expected	Expected	Expected
Rat	Tested	Tested	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

15 products for Alternative Product

7 products for Alternative Version

Target data

See full target information NGFR

Function

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed:24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed:26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed:23785138).

Alternative names

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

Recommended products

Rabbit Recombinant Monoclonal p75 NGF Receptor antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 10 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EP1039Y
Purification technique: Affinity purification Protein A
Specificity: The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Dissociation constant: 3.25 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab221212 is the carrier-free version of Anti-p75 NGF Receptor antibody [EP1039Y] ab52987.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

Associated diseases and disorders

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Image from Abe SI. et al. PLoS One. 2017 Nov 30;12(11):e0188705. doi: 10.1371/journal.pone.0188705.
Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
The differentiation capacity of purified CD34⁺ cells cultured for 3 days in the presence or absence of ALK5i was evaluated by performing immunofluorescence analysis assessing whether CD34⁺ cells had changed to cells expressing p75 and/or α-SMA. Expressions of CD34, p75 and α-SMA were assessed by immunofluorescence on day 0 (F-H), day 3 in SP+f medium (I-K), or day 3 in the same medium as (I-K) but with ALK5i (L-N).
Cultured re-aggregates were fixed in 4% PFA and embedded in paraffin. Sections (5 ∝m) were boiled in 0.01 M citrate (pH 6.0) with 0.1% Tween 20 for 10 min, washed three times in 0.1% Tween-20/PBS, transferred to blocking solution containing 5% BSA and 5% horse serum (Sigma) or goat serum (Invitrogen) in 0.1% Triton X-100/PBS for 1 hr, and incubated with primary antibody (p75 at 1/100 dilution) at 4°C overnight. After washing, the secondary antibody was added, and the sections were incubated for 2 hrs at room temperature. Microscopic images were obtained using a CCD camera (DP72, Olympus, Tokyo) mounted on a fluorescence microscope (BX60, or BX61VS-ASW, Olympus). Cultured cells on coverglasses were fixed in 4% PFA. Antigen retrieval was done by incubation with 100% methanol (-20°C) 10 min, and 0.3% Triton X-100 for 10 min.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
Lane 1 (input): PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10μg
Lane 2 (+): PC-12 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 in PC-12 whole cell lysate

Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 immunoprecipitating p75 NGF receptor in PC-12 whole cell lysates. For western blotting, primary antibody used was Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 at 1.6 μg/ml. VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution. Capture antibody was used at 1:40 dilution (2μg in 0.35mg lysates).
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure: 10 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
All lanes: Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987)
Predicted band size: 45 kDa
Observed band size: 75 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
Purified Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 staining p75 NGF receptor in paraffin embedded Human tonsil tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 3.3μg/ml. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on germinal centre of human tonsil.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
Image from Li Y et al. Reprod Biol Endocrinol. 2011 Mar 8;9:30. Fig 2.; doi:10.1186/1477-7827-9-30; 8 March 2011 Reproductive Biology and Endocrinology 2011 9:30.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
Immunohistochemical analysis of murine uterus tissue with adenomyosis, staining p75 NGF Receptor with Anti-p75 NGF Receptor antibody [EP1039Y] ab52987.

Antigen retrieval was performed by heat mediation in citrate buffer (pH 6). Tissue was blocked with goat serum for 15 minutes before incubating with primary antibody (1/100) overnight at 4°C. A biotinylated goat anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
Flow Cytometry (Intracellular) - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
Intracellular Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with purified Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 at 1/80 dilution (1ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
Purified Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 staining p75 NGF receptor in PC-12 (rat adrenal gland pheochromocytoma) by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 3.9 µg/ml. An AlexaFluor^®488 Goat anti-Rabbit was used as the secondary antibody at 2 µg/ml. DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic and Membranous staining in PC-12 cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
Flow Cytometry (Intracellular) - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
Intracellular Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with unpurified Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 at 1/60 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
ICC/IF image of Anti-p75 NGF Receptor antibody [EP1039Y] ab52987 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987, 1 µg/mL) overnight at 4^oC. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluo^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.4 µM.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [EP1039Y] ab52987).
OI-RD Scanning - Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.