Mouse Monoclonal p95/NBS1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat, Transfected cell lysate, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NBN aa 450-650.
Images
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (AB181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img174729.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [7E4A2] (AB181729)")
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (AB181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img103408.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [7E4A2] (AB181729)")
![Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody [7E4A2] (AB181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--immunocytochemistry-immunofluorescence-img53163.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody [7E4A2] (AB181729)")
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (AB181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img163136.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [7E4A2] (AB181729)")
![Flow Cytometry - Anti-p95/NBS1 antibody [7E4A2] (AB181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--flow-cytometry-img121479.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-p95/NBS1 antibody [7E4A2] (AB181729)")
Publications
Filter by
- Science advances 7:2021GRB2 enforces homology-directed repair initiation by MRE11.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZu Ye,Shengfeng Xu,Yin Shi,Albino Bacolla,Aleem Syed,Davide Moiani,Chi-Lin Tsai,Qiang Shen,Guang Peng,Paul G Leonard,Darin E Jones,Bin Wang,John A Tainer,Zamal AhmedPubMed 34348893
- Drug design, development and therapy 15:1749-17632021MYC/NBS1-Mediated DNA Damage Response is Involved in the Inhibitory Effect of Hydroxysafflor Yellow A on Glioma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongfang Tang,Tao Huang,Qilong Tian,Julei WangPubMed 33953544
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human NBN aa 450-650. The exact immunogen used to generate this antibody is proprietary information. Database link O60934
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected | Expected |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell lysate | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Transfected cell lysate | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Recombinant fragment - Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:10888888, PubMed:15616588, PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:19759395, PubMed:23115235, PubMed:28216226, PubMed:28867292, PubMed:9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:19759395, PubMed:28867292, PubMed:9705271). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:19759395, PubMed:9705271). The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination (PubMed:19759395, PubMed:28867292, PubMed:9705271). Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites (PubMed:12419185, PubMed:15616588, PubMed:18411307, PubMed:18582474, PubMed:18583988, PubMed:18678890, PubMed:19759395, PubMed:19804756, PubMed:23762398, PubMed:24534091, PubMed:27814491, PubMed:27889449, PubMed:33836577). Recruits MRE11 and RAD50 components of the MRN complex to DSBs in response to DNA damage (PubMed:12419185, PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:24534091, PubMed:26438602). Promotes the recruitment of PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites, activating their functions (PubMed:15064416, PubMed:15616588, PubMed:15790808, PubMed:16622404, PubMed:22464731, PubMed:30952868, PubMed:35076389). Mediates the recruitment of phosphorylated RBBP8/CtIP to DSBs, leading to cooperation between the MRN complex and RBBP8/CtIP to initiate end resection (PubMed:19759395, PubMed:27814491, PubMed:27889449, PubMed:33836577). RBBP8/CtIP specifically promotes the endonuclease activity of the MRN complex to clear DNA ends containing protein adducts (PubMed:27814491, PubMed:27889449, PubMed:30787182, PubMed:33836577). The MRN complex is also required for the processing of R-loops (PubMed:31537797). NBN also functions in telomere length maintenance via its interaction with TERF2: interaction with TERF2 during G1 phase preventing recruitment of DCLRE1B/Apollo to telomeres (PubMed:10888888, PubMed:28216226). NBN also promotes DNA repair choice at dysfunctional telomeres: NBN phosphorylation by CK2 promotes non-homologous end joining repair at telomeres, while unphosphorylated NBN promotes microhomology-mediated end-joining (MMEJ) repair (PubMed:28216226). Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex (PubMed:23762398).
Alternative names
NBS, NBS1, P95, NBN, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, hNbs1
Recommended products
Mouse Monoclonal p95/NBS1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat, Transfected cell lysate, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NBN aa 450-650.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human NBN aa 450-650. The exact immunogen used to generate this antibody is proprietary information. Database link O60934
- Clone number
- 7E4A2
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to supernatant. Lot no's high than GR150721-8 are from Tissue Culture Supernatant.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target known as p95/NBS1 also referred to as NBN protein is a critical component in the DNA damage response mechanism. The full molecular weight of the NBS1 protein is approximately 95 kDa. This protein is abundantly expressed in various tissues particularly where there is a higher rate of cell division or repair such as in the thymus and testis. It plays an important role in maintaining the stability of the genome through its involvement in the repair of double-strand breaks.
- Biological function summary
The NBS1 protein functions as an essential component of the MRN complex which also includes MRE11 and RAD50 proteins. This complex is fundamental in accurately detecting DNA double-strand breaks and initiating repair processes. Through its actions NBS1 facilitates numerous cellular processes that preserve genomic integrity such as homologous recombination and non-homologous end joining. Its influence in controlling the cell cycle further emphasizes its role in maintaining cellular health.
- Pathways
The NBS1 protein engages significantly in the DNA damage response and repair pathways notably impacting the ATM signaling pathway. By interacting with proteins such as ATM kinase NBS1 facilitates the phosphorylation and activation of several downstream effectors necessary for DNA repair and cell cycle checkpoints. The MRN complex also links with the RAD50 protein highlighting its involvement in these pathways.
- Associated diseases and disorders
Mutations in the NBS1 gene are linked to Nijmegen breakage syndrome characterized by sensitivity to radiation immune deficiency and increased cancer risk. Additionally NBS1's role in pathways makes it pertinent to certain cancer pathologies where its malfunction can contribute to genomic instability. The NBN protein through its interaction with ATM and RAD50 assists in understanding its connection with predispositions to these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img174729.jpg "Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
False colour image of Western blot: Anti-p95/NBS1 antibody [7E4A2] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181729 was shown to bind specifically to p95/NBS1. A band was observed at 95 kDa in wild-type A431 cell lysates with no signal observed at this size in NBN knockout cell line Human NBN (p95/NBS1) knockout A-431 cell line ab269506 (knockout cell lysate Human NBN (p95/NBS1) knockout A-431 cell lysate ab269668). To generate this image, wild-type and NBN knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
All lanes: Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729) at 1/500 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: NBN knockout A431 cell lysate at 20 µg
Lane 2: Western blot - Human NBN (p95/NBS1) knockout A-431 cell line (Human NBN (p95/NBS1) knockout A-431 cell line ab269506)
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 95 kDa
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img103408.jpg "Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
All lanes: Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729) at 1/500 dilution
Lane 1: A549 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: PC-12 cell lysate
Predicted band size: 84 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--immunocytochemistry-immunofluorescence-img53163.jpg "Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
Immunofluorescent analysis of HeLa cells labeling p95/NBS1 with ab181729 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img163136.jpg "Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
All lanes: Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729) at 1/500 dilution
Lane 1: non-transfected HEK293 cell lysate
Lane 2: p95/NBS1 (aa 467-615)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 84 kDa
![Flow Cytometry - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--flow-cytometry-img121479.jpg "Flow Cytometry - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Flow Cytometry - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
Flow Cytometrical analysis of HeLa cells labeling p95/NBS1 with ab181729 at 1/200 (green) compared to a negative control antibody (red).
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img137615.jpg "Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
All lanes: Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729) at 1/500 dilution
All lanes: p95/NBS1 (aa 467-615) human recombinant protein
Predicted band size: 84 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114872.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
Immunohistochemical analysis of paraffin embedded Human rectum cancer tissue labeling p95/NBS1 with ab181729 at 1/200 with DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120582.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729)
Immunohistochemical analysis of paraffin embedded Human cervical cancer tissue labeling p95/NBS1 with ab181729 at 1/200 with DAB staining.
![Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--western-blot-img137615.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114872.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-7e4a2-ab181729--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120582.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4A2] (ab181729)")
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